| Project/Area Number |
23659294
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | University of Toyama |
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Principal Investigator |
KITAJIMA Isao 富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)
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| Co-Investigator(Kenkyū-buntansha) |
NIIMI Hedeki 富山大学, 附属病院, 助教 (50401865)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 転写因子 / NF-kappa B / FCS法 / ハイスープット / 1分子計測法 / 救急医療 / ハイスループット検査 / NF-ｋB活性化 / 蛍光相関分光法(FCS) / 病院検査室 / 炎症反応 / NF-kＢ活性化 / 蛍光相関分光法 |
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| Research Abstract |
We developed new inflammation diagnostic system in order to raise the lifesaving rate of systemic inflammatory reactive syndrome, SIRS. Using the eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.Further, we have established a novel high-throughput measurement system for the NF-κB activity using fluorescence correlation spectroscopy （FCS), which was a methodology to examine the size and number of fluorescence-labeled molecules in a confocal area by direct observation of Brownian motion in solutions, because the elevation of NF-κB activity reflects the acute inflammation. Measuring the NF-κB activity in the patients with SIRS would be useful as inflammatory marker for emergency care.
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