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Application to the emergency care by establishment of high-throughput examination systems for transcription factor NF-κB activation

Research Project

Project/Area Number 23659294
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionUniversity of Toyama

Principal Investigator

KITAJIMA Isao  富山大学, 大学院・医学薬学研究部(医学), 教授 (50214797)

Co-Investigator(Kenkyū-buntansha) NIIMI Hedeki  富山大学, 附属病院, 助教 (50401865)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords転写因子 / NF-kappa B / FCS法 / ハイスープット / 1分子計測法 / 救急医療 / ハイスループット検査 / NF-kB活性化 / 蛍光相関分光法(FCS) / 病院検査室 / 炎症反応 / NF-kB活性化 / 蛍光相関分光法
Research Abstract

We developed new inflammation diagnostic system in order to raise the lifesaving rate of systemic inflammatory reactive syndrome, SIRS. Using the eukaryote-made thermostable DNA polymerase, the sensitive and reliable detection of bacteria becomes feasible for large fields, thereby making the development of a wide range of powerful applications possible.Further, we have established a novel high-throughput measurement system for the NF-κB activity using fluorescence correlation spectroscopy (FCS), which was a methodology to examine the size and number of fluorescence-labeled molecules in a confocal area by direct observation of Brownian motion in solutions, because the elevation of NF-κB activity reflects the acute inflammation. Measuring the NF-κB activity in the patients with SIRS would be useful as inflammatory marker for emergency care.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2012 2011

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (14 results) (of which Invited: 1 results) Book (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Study of hemostatic biomarkers in acute ischemic stroke by clinical subtype2012

    • Author(s)
      Hirano K, Takashima S, Dougu N, Taguchi Y, Nukui T, Konishi H, Toyoda S, Kitajima I, Tanaka K.
    • Volume
      5021(5)
    • Pages
      404-410
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 久保田智美、林史朗、仁井見英樹、北島勲出動向調査2012

    • Author(s)
      久保田智美、林史朗、仁井見英樹、北島勲
    • Journal Title

      臨床病理

      Volume: 60(7) Pages: 605-611

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Study of hemostatic biomarkers in acute ischemic stroke by clinical subtype.2012

    • Author(s)
      Hirano K,
    • Journal Title

      J. Stroke Cerebrovasc Dis.

      Volume: 50(11) Pages: 404-410

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physical and Functional Interaction of the Active Zone Protein CAST/ERC2 and the b-subunit of the Voltage-dependent Ca2+ Channel.2012

    • Author(s)
      Kiyonaka
    • Journal Title

      The Journal of Biochemistry

      Volume: 152(2) Pages: 149-159

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Trend survey of ocular infections with bacteria at Toyama    University Hospital over the past six years--from the standpointof laboratory examination2012

    • Author(s)
      Kubota T
    • Journal Title

      Rinsho Byori

      Volume: 60(7) Pages: 605-611

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel "eukaryote-made" thermostable DNA polymerase which is free from bacterial DNA contamination2011

    • Author(s)
      Niimi H, Mori M, Tabata H, Minami H, Ueno T, Hayashi S, Kitajima I
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 49(9) Pages: 3316-3320

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of serine/threonine kinase SAD-B in mouse pericheral nerve synapse2011

    • Author(s)
      Hagiwara A, Harada K, Hida Y, Kitajima I, Ohtsuka T
    • Journal Title

      NeuroReport

      Volume: 22(7) Pages: 319-325

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] (Japanese collaborative research group on "Acquired haemorrha-philia due to factor XIII deficiency): As many as 12 cases with haemorragic acquired factor XIII deficiency due to its inhibitors were recently found in Japan2011

    • Author(s)
      Ichinose A, Souri M, Kitajima I
    • Journal Title

      Thromb Haemost

      Volume: 105(5) Pages: 925-927

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel Y231del mutation of HFE in hereditary hemochromatosis provides in vivo evidence that the Huh-7 is a human hemochromatotic cell line2011

    • Author(s)
      Takano A, Niimi H, Atarashi Y, Sawasaki T, Terasaki T, Nakabayashi, T, Kitajima I, Tobe K, Takahara T
    • Journal Title

      Liver International

      Volume: 31(10) Pages: 1593-1594

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel "eukaryote-made" thermostable DNA polymerase which is free from bacterial DNA contamination2011

    • Author(s)
      Niimi H, Mori M, Tabata H, Minami H, Ueno T, Hayashi S,Kitajima I
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 49 Pages: 3316-3320

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distribution of serine/threonine kinase SAD-B in mouse pericheral nerve synapse2011

    • Author(s)
      Hagiwara A, Harada K, Hida Kitajima I, Ohtsuka T
    • Journal Title

      NeuroReport

      Volume: 22 Pages: 319-325

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel Y231del mutation of HFE in hereditary hemochromatosis provides in vivo evidence that the Huh-7 is a human hemochromatotic cell line.2011

    • Author(s)
      Takano A, Niimi H, Atarashi Y, Sawasaki T, Terasaki T, Nakabayashi, T, Kitajima I, Tobe K, Takahara T:
    • Journal Title

      Liver International

      Volume: 31 Pages: 1593-1594

    • Related Report
      2011 Research-status Report
  • [Presentation] A novel TM-mapping methodthat enables identification of pathogenic microorganisms within 3 hours after samples are collected2012

    • Author(s)
      Niimi H, Ueno T, Hayashi S, Mori M, Tabata H, Minami H, Saito S, Kitajima I
    • Organizer
      52ndInterscience Conference on Antimicrobial Agents and Chemotherapy
    • Place of Presentation
      San Francisco, CA, USA
    • Year and Date
      2012-09-11
    • Related Report
      2012 Final Research Report
  • [Presentation] 新しい臨床化学への挑戦‐新規酵母由来耐熱性DNA polymeraseの開発と転写因子NF-kappa B測定の検査室への応用を通じて‐2012

    • Author(s)
      北島勲
    • Organizer
      第30回日本臨床化学会甲信越支部総会
    • Place of Presentation
      松本
    • Year and Date
      2012-06-02
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel rapid method enables identification of pathogenic micro-organisms within 3 hours after samples are collected2012

    • Author(s)
      Niimi H, Ueno T, Hayashi S, Mori M, Tabata H, Minami H, Kitajima I
    • Organizer
      22ndEuropean Congress of Clinical Microbiology and Infectious Diseases
    • Place of Presentation
      London, UK
    • Year and Date
      2012-04-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 菌血症/全身炎症反応症候群に対する迅速高感度検査システムの構築2012

    • Author(s)
      北島勲
    • Organizer
      平成23年度臨床検査医学研究振興基「小酒井望賞」受賞講演
    • Place of Presentation
      東京
    • Year and Date
      2012-02-03
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel TM-mapping methodthat enables identification of pathogenic microorganisms within 3 hours after samples are collected.2012

    • Author(s)
      Niimi H and Kitajima I
    • Organizer
      52nd Interscience Conference on Antimicrobial Agents and Chemotherapy
    • Place of Presentation
      San Francisco,USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新しい臨床化学への挑戦‐新規酵母由来耐熱性DNA polymeraseの開発と転写因子NF-kappa B測定の検査室への応用を通じて‐2012

    • Author(s)
      北島勲
    • Organizer
      第30回日本臨床化学会甲信越支部総会
    • Place of Presentation
      松本市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] A novel rapid method enables identification of pathogenic micro-organisms within 3 hours after samples are collected.2012

    • Author(s)
      Niimi H and Kitajima I
    • Organizer
      22nd European Congress of Clinical Microbiology and Infectious Diseases
    • Place of Presentation
      London,UK
    • Related Report
      2012 Annual Research Report
  • [Presentation] Post-marketing surveillance of the safety and effectiveness of thrombomodulin alfa in Japanease patients with DIC2011

    • Author(s)
      Sakata Y, Mimuro J, Takahashi H, Tshuji H, Eguchi Y, Kitajima I, Matsushita T, Kuroda T
    • Organizer
      XXIII Congress of the International Society of Thrombosis and Haemostasis
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2011-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 転写因子NF-κBから斬る炎症、がん、血液凝固のクロストーク2011

    • Author(s)
      北島勲
    • Organizer
      第12回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      富山
    • Year and Date
      2011-06-17
    • Related Report
      2012 Final Research Report
  • [Presentation] 菌血症/全身炎症反応症候群に対する迅速・高感度検査システムの構築2011

    • Author(s)
      北島勲
    • Organizer
      第22回日本臨床化学会九州支部総会・第56回日本臨床検査医学会九州地方会合同会
    • Place of Presentation
      福岡
    • Year and Date
      2011-02-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Rapid change of the finbrin monomer complex level during the perioperative period for early diagnosis of venous thrombolism2011

    • Author(s)
      Kitajima I, Yoshioka K, Kabata T, Tani M, Tomita K, Uji Y
    • Organizer
      21stInternatinal Congress of Clinical Chemistry and Laboratory Medicine
    • Place of Presentation
      Berlin,Germany
    • Related Report
      2012 Final Research Report
  • [Presentation] NF-・B activity in the peripheral blood lymphocytes correlates with metabolic syndrome-related biomarkers in patients with essential hypertension2011

    • Author(s)
      Kitajima I, Harada K, Tomoda F, KagitaniS, KoikeT, Inoue H
    • Organizer
      2011 American Society for Clinical Pathology Annual Meeting/WASPaLM XXVI Wordld Cobgress
    • Place of Presentation
      Las Vegas, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Highly sensitive detection of bacteria using novel "eukaryote-made" Taq polymerase2011

    • Author(s)
      Niimi H, Mori M, Minami H, Ueno T, Hayashi S, Kitajima I
    • Organizer
      21stInternatinal Congress of Clinical Chemistry and Laboratory Medicine
    • Place of Presentation
      Berlin,Germany
    • Related Report
      2012 Final Research Report
  • [Presentation] NF-kB activity in the peripheral blood lymphocytes correlates with metabolic syndrome-related biomarkers in patients with essential hypertension.2011

    • Author(s)
      Kitajima I, Harada K, Tomoda F, Kagitani S, Koike TInoue H
    • Organizer
      2011 American Society for Clinical Pathology Annual Meeting/WASPaLM XXVI Wordld Congress,
    • Place of Presentation
      Las Vegas, USA
    • Related Report
      2011 Research-status Report
  • [Book] 凝固・線溶と臨床検査。わかりやすい血栓と止血の臨床(日本血栓止血学会編集)2011

    • Author(s)
      北島勲
    • Publisher
      南江堂、東京
    • Related Report
      2012 Final Research Report
  • [Book] プロトロンビン時間(PT), トロンボテスト(TT),ヘパプラスチンテスト(HPT),活性化部分トロンボプラスチン時間(APTT)。臨床検査データブック2011-2012(黒川清、春日正雅、北村聖編集)2011

    • Author(s)
      北島勲
    • Total Pages
      279
    • Publisher
      医学書院、東京
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] Method for identifying pathogenic microorganisms responsible for infection by extracting the DNA of a microorganism2012

    • Inventor(s)
      Miimi H, Kitajima I
    • Industrial Property Rights Holder
      富山大学
    • Acquisition Date
      2012-12-04
    • Related Report
      2012 Final Research Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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