| Project/Area Number |
23659295
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshihisa 浜松医科大学, 医学部, 助教 (00362187)
SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664)
NAKAMURA Toshio 浜松医科大学, 医学部, 准教授 (40283353)
SUGIMURA Haruhiko 浜松医科大学, 医学部, 教授 (00196742)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 循環腫瘍細胞 / オミックス解析 / リピドミクス / 次世代シーケンサーーケンサー / 次世代シーケンサー |
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| Research Abstract |
We investigated primary focus, metastatic focus and circulating tumor cells (CTC) in colorectal cancer (CRC) patients by genomics approach in order to deve lop a novel clinical laboratory testing. CRC cell lines were mixed with peripheral blood from healthy volunteer, and isolated from the mixture. Captured by magnetic cell separation and flow cytometry, the cancer cell lines were collected and confirmed by immunostaining with anti-cytokeratin antibody. The technologies were established, however, CTC could not be isolated from CRC patients in early stage. We implemented iTRAQ (isobaric tags for relative and absolute quantitation) method to develop a novel CRC biomarker, and found LRPPRC (Leucine rich PPR motif containing protein) that was expressed 1.62 fold higher in cancer tissues. Tissue-Microarray and immunohistochemistry revealed that the expression level of LRPPRC was related to cancer differentiation. LRPPRC could be a novel marker for prognosis prediction.
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