Development of diagnosis test kits with high specificity, high sensitivity, and low cost against tropical infectious diseases.

• Project/Area Number: 23659297
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Osaka University
• Principal Investigator: IKUTA Kazuyoshi 大阪大学, 微生物病研究所, 教授 (60127181)
• Co-Investigator(Kenkyū-buntansha): KUROSU Takeshi 大阪大学, 微生物病研究所, 助教 (70432432) ; SAKUDO Akikazu 琉球大学, 医学部保健学科, 准教授 (10397672)
• Research Collaborator: OKABAYASHI Tamaki 大阪大学, 微生物病研究所, 特任准教授 (10359995) ; SASAKI Tadahiro 大阪大学, 微生物病研究所, 特任講師 (20547533) ; KOSAKA Mieko アルフレッサファーマ株式会社, 診断薬研究開発部, 室長 (70592278) ; NAKAISHI Kazunari 株式会社 BL, 商品開発部, 部長
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 臨床微生物学 / 迅速診断 / デングウイルス / チクングンヤウイルス / 迅速診断キット / イミュノクロマト法 / ヒト単クローン抗体 / マウス単クローン抗体 / タイ / 熱帯感染症 / イムノクロマトキット / 蚊媒介性 / 単クローン抗体 / 国際研究者交流

Research Abstract

Tropical infections are especially endemic in low-income populations in developing regions. Dengue disease that is induced by dengue virus is difficult for differential diagnosis from other fabric infectious diseases. The aim of this study, we developed immune-chromatograph rapid test kit against dengue, chikungunya, and influenza virus infections with high sensitivity, high specificity, low cost, and simple use at clinics.

Research Products

• [Journal Article] Characterization of chikungunya virus infection of a human keratinocyte cell line: Role of mosquito salivary gland protein in suppressing the host immune response. (2013)
  • Author(s): Puiprom O, Morales Vargas RE, Potiwat R, Chaichana P, Ikuta K, Ramasoota P, Okabayashi T.
  • Journal Title: Infect Genet Evol Volume: 17C Pages: 210-215
  • DOI: 10.1016/j.meegid.2013.04.005
  • Peer Reviewed
• [Journal Article] Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection. (2013)
  • Author(s): Sasaki T, Setthapramote C, Kurosu T, et al
  • Journal Title: Antiviral Res. Volume: S0166-3542(13) Issue: 3 Pages: 72-7
  • DOI: 10.1016/j.antiviral.2013.03.018
  • Peer Reviewed
• [Journal Article] Chikungunya virus induces a more moderate cytopathic effect in mosquito cells than in mammalian cells. (2013)
  • Author(s): Li, Y.G., Siripanyaphinyo, U., Tumkosit, U., Noranate, N., A-Nuegoonpipat, A., Kurosu, T., Ikuta, K., Takeda, N., and Anantapreecha, S.
  • Journal Title: Intgervirology Volume: 56 Issue: 1 Pages: 6-12
  • DOI: 10.1159/000339985
  • Peer Reviewed
• [Journal Article] Reliability of a newly-developed immunochromatography diagnostic kit for pandemic influenza A/H1N1pdm virus: Implications for drug administration. (2012)
  • Author(s): Sasaki, T., Kubota-Koketsu, R., Takei, M., Hagihara, T., Iwamoto, S., Murao, T., Sawani, K., Fukae, D., et al.
  • Journal Title: PLoS One Volume: 7 Issue: 11 Pages: e50670-e50670
  • DOI: 10.1371/journal.pone.0050670
  • Peer Reviewed
• [Journal Article] Limited cross-reactivity of mouse monoclonal antibodies against dengue virus capsid protein among four serotypes. (2012)
  • Author(s): Noda, M., Masrinoul, P., Pipattanaboon, C., Ramasoota, P., Setthapramote, C., Sasaki, T., Sasayama, M., Yamashita, A., Kurosu, T., Ikuta, K., and Okabayashi, T.
  • Journal Title: Biologics Volume: 6 Pages: 409-416
  • DOI: 10.2147/btt.s37792
  • Peer Reviewed
• [Journal Article] Human monoclonal antibodies to neutralize all dengue virus serotypes using lymphocytes from patients at acute phase of the secondary infection. (2012)
  • Author(s): Setthapramote C, Sasaki T, Puiprom O, et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 423(4) Issue: 4 Pages: 867-872
  • DOI: 10.1016/j.bbrc.2012.06.057
  • Peer Reviewed
• [Journal Article] Analysis of Vis-NIR spectra changes to measure the inflammatory response in the nasal mucosal region of influenza A and B virus-infected patients. (2012)
  • Author(s): Sakudo, A., Baba, K., and Ikuta, K.
  • Journal Title: J. Clin. Virol. Volume: 55 Issue: 4 Pages: 334-338
  • DOI: 10.1016/j.jcv.2012.08.015
  • Peer Reviewed
• [Journal Article] Discrimination of influenza virus-infected nasal fluids by Vis-NIR spectroscopy. (2012)
  • Author(s): Sakudo, A., Baba, K., and Ikuta, K.
  • Journal Title: Clin. Chim. Acta Volume: 414C Pages: 130-134
  • DOI: 10.1016/j.cca.2012.08.022
  • Peer Reviewed
• [Journal Article] Poly(I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. (2012)
  • Author(s): Li, Y.G., Siripanyaphinyo, U., Tumkosit, U., Noranate, N., A-Nuegoonpipat, A., Pan, Y., Kameoka, M., Takeshi, K., Ikuta, K., Takeda, N., and Anantapreecha, S.
  • Journal Title: Virol. J. Volume: 9 Issue: 1 Pages: 114-114
  • DOI: 10.1186/1743-422x-9-114
  • Peer Reviewed
• [Journal Article] Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip (2012)
  • Author(s): Naoki Nagatani, Keiichiro Yamanaka, Hiromi Ushijima, Ritsuko Koketsu, Tadahiro Sasaki, Kazuyoshi Ikuta, Masato Saito, Toshiro Miyahara and Eiichi Tamiya
  • Journal Title: Analyst Volume: (In press) Issue: 15 Pages: 3422-3426
  • DOI: 10.1039/c2an16294f
  • Peer Reviewed
• [Journal Article] Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1 (2011)
  • Author(s): Mizuike, R., Sasaki, T, Baba, K., Iwamoto, H., Shibai, Y., Kosaka, M., Kubota-Koketsu, R., Yang, C.-S., Du, A., Sakudo, A., Tsujikawa, M., Yunoki, M., Ikuta, K
  • Journal Title: Clin.Vaccine Immunol. Volume: 18 Issue: 3 Pages: 494-499
  • DOI: 10.1128/cvi.00269-10
  • Peer Reviewed
• [Journal Article] Co-existence of major and minor viral populations from two different origins in patients secondarily infected with dengue virus serotype 2 in Bangkok (2011)
  • Author(s): Puiprom O., Yamashita A., Sasayama M., Limkittikul K., Boonha K., Jittmitraphap A., Leaungwutiwong P,. Kurosu T., Ramasoota P., Ikuta K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 413 Issue: 1 Pages: 136-142
  • DOI: 10.1016/j.bbrc.2011.08.069
  • Peer Reviewed
• [Journal Article] Capture of dengue virus type 3 using anionic polymer-coated magnetic beads (2011)
  • Author(s): Sakudo A, Masrinoul P, Tanaka Y, Ikuta K
  • Journal Title: Int J Mol Med Volume: 28 Pages: 625-628
  • DOI: 10.3892/ijmm.2011.733
  • Peer Reviewed
• [Journal Article] Highly conserved region 141-168 of the NS1 protein is a new common epitope region of dengue virus. (2011)
  • Author(s): Masrinoul, P., Diata, M. O., Pambudi, S., Limkittikul, K., Ikuta, K., and Kurosu, T.
  • Journal Title: Jpn. J. Infect. Dis. Volume: 64 Pages: 109-115
  • Peer Reviewed
• [Presentation] シーズンの大阪府下におけるインフルエンザ様疾患患者検体を用いた Pandemic A(H1N1)2009迅速診断キットの評価 (2012)
  • Author(s): 佐々木正大
  • Organizer: 第53回日本臨床ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-06-17
• [Presentation] 抗体医薬開発を目的としたデングウイルス感染症に対する抗デングヒト型単クローン抗体の作製 (2012)
  • Author(s): 佐々木正大、Chayanee Setthapramote、Pasnnamthip Pitsksajjakul、Chonlatip Pipattanaboon、et a.
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
• [Presentation] 抗デングウイルス抗体とエンベロープ蛋白質との相互作用解析 (2012)
  • Author(s): 西村光弘、佐々木正大、沖大也、チャヤニーセタプラモーテ、小野健一郎、ほか
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
• [Presentation] Analysis of antibodies against dengue virus NS1 from mouse and dengue virus infected patients. (2012)
  • Author(s): Omokoko Magot, aaaaaaamasrinoul Promsin, Setthapramote Chayanee, 佐々木正大、ほか
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
• [Presentation] Cross-reasctive antibody responses with host cells by BALB/c mice immunized with BALB/c-derived cells infected with chikungunya virus, but not with those with dengue virus. (2011)
  • Author(s): Masrinou, P., Punkum, C., Noda, M., Kurosu, T., Ikuta, K., and Okabayashi, T.
  • Organizer: Joint International Tropical Medicine Meeting 2011
  • Place of Presentation: Bangkok, Thailand
• [Remarks]
  • URL: http://virology.biken.osaka-u.ac.jp/
• [Remarks] マヒドン大学における医療関係者への迅速診断キットのセミナー (2011年7月27日)
• [Remarks] 大阪大学微生物病研究所ウイルス免疫分野
  • URL: http://virology.biken.osaka-u.ac.jp/
• [Remarks]
  • URL: http://virology.biken.osaka-u.ac.jp/virus/
• [Remarks]
  • URL: http://virology.biken.osaka-u.ac.jp/mocid/