| Project/Area Number |
23659302
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
KAI Masaaki 長崎大学, 医歯(薬)学総合研究科, 教授 (00160953)
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| Co-Investigator(Renkei-kenkyūsha) |
KABASHIMA Tsutomu 長崎大学, 大学院医歯薬学総合研究科, 准教授 (20274673)
SHIBATA Takayuki 長崎大学, 大学院医歯薬学総合研究科, 助教 (10448491)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | エイズ検査 / HIV / 変異 / 薬剤耐性 / プロテアーゼ / 基質特異性 / 蛍光反応 / ペプチド / エイズ / 薬物耐性 |
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| Research Abstract |
Acquired immunodeficiency syndrome (AIDS) is caused by HIV infection, and one of the most serious infectious diseases. Moreover, the emergence and transmission of drug resistance strains of HIV are the most serious problem in the world. Thus, new treatments and discrimination methods for drug resistance HIV are still in great need.
A noble assay method for HIV protease activity was developed, basing on a specific fluorescence derivatization reaction for peptides, previously. In this research, I tried to apply this HIV protease assay to discrimination for drug-resistant HIV proteases.
Consequently, mutant HIV proteases with drug-resistant could be directly discriminated by the present discrimination method.
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