Studies on rapid detection methods for multi-drug resistant bacteria by using specific antibodies

• Project/Area Number: 23659306
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kitasato University
• Principal Investigator: OKAMOTO Ryoichi 北里大学, 医学部, 講師 (30224083)
• Co-Investigator(Kenkyū-buntansha): TAKAYAMA Yoko 北里大学, 医学部, 講師 (80286278)
• Project Period (FY): 2011-04-28 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: カルバペネマーゼ / 特異抗体 / 多剤耐性菌 / 迅速診断法

Outline of Final Research Achievements

Multidrug-resistant gram-negative rods including Pseudomonas aeruginosa, Acinetobacter spp. and Enterobacteriaceae has become one of the most serious public health worldwide. These pathogens often produce carubapenemase including IMP-, VIM-, NDM- and KPC-types. We tiried (attemped) to develop a easy and rapid detection method of carbapenemae-producing Gram-negative rods by using specific-antibodie.

Research Products

• [Presentation] 当院の腸内細菌におけるカルバペネマーゼ産生菌の分離状況 (2014)
  • Author(s): 高山陽子、安達譲、二本柳伸、平田泰良、中村正樹、砂川慶介、岡本了一
  • Organizer: 第62回日本化学療法学会総会
  • Place of Presentation: ヒルトン福岡シーホーク（福岡市）
  • Year and Date: 2014-06-18
• [Presentation] 当院におけるカルバペネム系薬剤に感受性を示すカルバペネマーゼ産生菌の動向 (2011)
  • Author(s): 高山陽子, 安達譲, 二本柳伸, 中崎信彦, 平田泰良, 砂川慶介, 岡本了一
  • Organizer: 第59回日本化学療法学会総会
  • Place of Presentation: 札幌市
• [Presentation] Improved performance of the modified hodge test using the Mueller-Hinton agar supplemented with cloxacillin for screening carbapenemase-producing clinical isolates in Enteorbacteriaceae. (2011)
  • Author(s): Takayama, Y., Adachi, Y., Nihonnyanagi, S., Takagi, Y., Fujiki, K., Hirata, Y., Sunakawa, K., Okamoto, R.
  • Organizer: 51st Interscience Conference on Antimicrobial Agents and Chemotherapy
  • Place of Presentation: Chicago(USA)
• [Presentation] カルバペネマーゼ産生菌を検出するModified Hodge Test (MHT) の改良について (2011)
  • Author(s): 高山陽子, 平田泰良, 二本柳伸, 砂川慶介, 岡本了一
  • Organizer: 第58回日本化学療法学会東日本支部総会
  • Place of Presentation: 山形市