Analysis of novel autoantigens with labile epitopes on cell surface, and development of new diagnostic test for the detection of autoantibodies
| Project/Area Number |
23659312
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
MIURA Keiji 藤田保健衛生大学, 医療科学部, 講師 (20199946)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Shunji 藤田保健衛生大学, 医学部, 教授 (00166951)
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| Research Collaborator |
TAKAHASHI Kazuo 藤田保健衛生大学, 医学部, 講師 (90631391)
IWATA Yohei 藤田保健衛生大学, 医学部, 講師 (60437861)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 自己免疫疾患 / 自己抗体 / 自己抗原 / 血管内皮細胞 / 細胞表面抗原 / 膜タンパク / 診断法 / ELISA / 腎炎 / 臨床検査 |
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| Outline of Final Research Achievements |
We developed a new assay for detecting AECA by CSP-ELISA. IgG-AECA positive rates of using CSP-ELISA were 95% for SLE, 97% for MCTD and 58% for SSc. Also, IgA-AECA positively rates were 34%, 97%, and 58% respectively. AECA in patients of these disorders recognized membrane proteins expressed on cell surface of endothelial cells. CSP-ELISA holds promise for improving the detection of anti-cell surface autoantibodies and the diagnosis in the clinical context.
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Report
(5 results)
Research Products
(8 results)
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[Presentation] The Level of IgA Antibodies to Endothelial Cells Correlate with Histological Evidence of Disease Activities in Patients with Lupus Nephritis2014
Author(s)
Ayako Kondo, Kazuo Takahashi, Tomohiro Mizuno, Daisuke Hirano, Shinichi Akiyama, Hiroki Hayashi, Shigehisa Koide, Hiroshi Takahashi, Midori Hasegawa, Yoshiyuki Hiki, Shunji Yoshida, Keiji Miura, Yukio Yuzawa
Organizer
American Society of Nephrology, Annual Meeting
Place of Presentation
Phiradelphia, USA
Year and Date
2014-11-11 – 2014-11-16
Related Report
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[Presentation] Autoantibodies Directed Against Cell Surface Components in Autoimmune Disease Patients: Proposal of a Novel ELISA for the Detection of Anti-Endothelial Cell Antibodies.2013
Author(s)
Miura, K., Kondo, A., Takahashi, K., Hirano, D., Hiki, Y., Yoshida, S., Yuzawa, Y. and Kurosawa, Y.
Organizer
American College of Rheumatology, Annual Meeting.
Place of Presentation
SanDiego. USA.
Related Report
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[Presentation] IgA antibodies to glomerular endothelial cells in patients with lupus nephritis: correlations with renal injury2013
Author(s)
Takahasshi, K., Kodo, A., Hirano, D., Akiyama, S., Hayashi, H., Koide, S., Hasegawa, M., Yoshida, S., Hiki, Y., Miura, K., and Yuzawa, Y.
Organizer
American Society of Nephrology, Annual Meeting.
Place of Presentation
Atlanta. USA.
Related Report
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