| Project/Area Number |
23659312
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
MIURA Keiji 藤田保健衛生大学, 医療科学部, 講師 (20199946)
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIDA Shunji 藤田保健衛生大学, 医学部, 教授 (00166951)
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| Research Collaborator |
TAKAHASHI Kazuo 藤田保健衛生大学, 医学部, 講師 (90631391)
IWATA Yohei 藤田保健衛生大学, 医学部, 講師 (60437861)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 自己免疫疾患 / 自己抗体 / 自己抗原 / 血管内皮細胞 / 細胞表面抗原 / 膜タンパク / 診断法 / ELISA / 腎炎 / 臨床検査 |
|---|
| Outline of Final Research Achievements |
We developed a new assay for detecting AECA by CSP-ELISA. IgG-AECA positive rates of using CSP-ELISA were 95% for SLE, 97% for MCTD and 58% for SSc. Also, IgA-AECA positively rates were 34%, 97%, and 58% respectively. AECA in patients of these disorders recognized membrane proteins expressed on cell surface of endothelial cells. CSP-ELISA holds promise for improving the detection of anti-cell surface autoantibodies and the diagnosis in the clinical context.
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