Development of a novel method for high-throughput discovery of trans- spliced RNAs in mammalian cells

• Project/Area Number: 23659313
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kurume University
• Principal Investigator: IMAMURA Rie 久留米大学, 医学部, 助教 (70309764)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 遺伝子検査学 / トランス・スプライシング / バイオテクノロジー

Research Abstract

Wedevelopedanovelmethodforhigh-throughputdiscoveryoftrans-splicedRNAsinmammalian cells. Our method is based on a mate-pair analysis, which enables us to collect information of both ends of DNA fragments. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNAs and those mate-pair fragments can be a cause of false positive trans-spliced genes. To solve this problem we developed a novel method, which allows us to prepare circularized DNA libraries in which each circularized DNA is originated from each single DNA. By this method, we can make a systematic survey of the presence of trans-spliced RNAs in mammalian cells in a highly precise manner, and we could find out several candidate genes in hematopoietic stem cells. This method is useful for discovery of trans-spliced RNAs and will enable us to understand the role of trans-spliced RNAs in mammalian.

Research Products

• [Journal Article] Successful treatment of small cell variant anaplastic large cell lymphoma with allogeneic peripheral blood stem cell transplantation, and review of the literature (2013)
  • Author(s): Imamura R, Mouri F, Oku E, Okamura T
  • Journal Title: Int J Hematol Volume: 97 Issue: 1 Pages: 139-143
  • DOI: 10.1007/s12185-012-1242-3
  • NAID: 10031144646
  • Peer Reviewed
• [Journal Article] A case of 8p11 myeloproliferative syndrome with BCR-FGFR1 gene fusion presenting with trilineage acute leukemia/lymphoma, successfully treated by cord blood transplantation (2013)
  • Author(s): Morishige S, Oku E, Mizuno S, Okamura T
  • Journal Title: Acta Haematol Volume: 129 Issue: 2 Pages: 83-89
  • DOI: 10.1159/000341289
  • Peer Reviewed
• [Journal Article] Unrelated cord blood transplantation for patients with adult T-cell leukemia/lymphoma: experience at a single institute (2012)
  • Author(s): Nakamura T, Oku E, Mizuno S, Okamura T
  • Journal Title: Int J Hematol Volume: 96 Issue: 5 Pages: 657-663
  • DOI: 10.1007/s12185-012-1177-8
  • NAID: 10031126764
  • Peer Reviewed
• [Journal Article] Amebic colitis and liver abscess complicated by high serum procalcitonin in acute myeloid leukemia. (2012)
  • Author(s): Oku E, Nomura K, Nakamura T, Morishige S, Seki R, Imamura R, Hashiguchi M, Osaki K, Mizuno S, Nagafuji K, Okamura T.
  • Journal Title: Kansenshogaku Zasshi. Volume: 86 Pages: 773-777
  • NAID: 10031176209
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/23367854
  • Peer Reviewed