| Project/Area Number |
23659313
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Kurume University |
|---|
Principal Investigator |
IMAMURA Rie 久留米大学, 医学部, 助教 (70309764)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 遺伝子検査学 / トランス・スプライシング / バイオテクノロジー |
|---|
| Research Abstract |
Wedevelopedanovelmethodforhigh-throughputdiscoveryoftrans-splicedRNAsinmammalian cells. Our method is based on a mate-pair analysis, which enables us to collect information of both ends of DNA fragments. In conventional mate-pair analysis, there must contain circularized DNAs derived from multiple DNAs and those mate-pair fragments can be a cause of false positive trans-spliced genes. To solve this problem we developed a novel method, which allows us to prepare circularized DNA libraries in which each circularized DNA is originated from each single DNA. By this method, we can make a systematic survey of the presence of trans-spliced RNAs in mammalian cells in a highly precise manner, and we could find out several candidate genes in hematopoietic stem cells. This method is useful for discovery of trans-spliced RNAs and will enable us to understand the role of trans-spliced RNAs in mammalian.
|
