Identification of frog cutaneous nociceptors and functional analysis of them using transgenic flies

• Project/Area Number: 23659321
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: Ohu University
• Principal Investigator: FURUYAMA Akira 奥羽大学, 歯学部, 助教 (80364454)
• Co-Investigator(Kenkyū-buntansha): SAITO Shigeru 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 細胞生理学研究部, 助教 (50422069) ; ISONO Kunio 東北大学, (連合)農学研究科(研究院), 産学連携研究員 (70124550) ; MASUZAWA Toru 茨城大学, 工学部, 教授 (40199691)
• Co-Investigator(Renkei-kenkyūsha): YONEHARA Norifumi 奥羽大学, 歯学部, 教授 (70124534)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2012: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 機械性侵害受容体 / ショウジョウバエ / カエル / TRPV1 / TRPV4 / TRPチャネル / 機械刺激 / 味覚神経 / 機械刺激受容体 / 痛覚受容体 / 味覚

Research Abstract

In this study, it was revealed that capsaicin induces mechanical allodinia but does not induce thermal allodinia in frog. It was suggested that ATP-P2X system might play an important role in induction of mechanical allodinia in frog, because P2X antagonist suramine blocked capsaicin-induced mechanical allodinia. We devised the nano oscillating impression equipment which used the elastic hinge mechanism to apply minute mechanical stimulation for taste organs (taste hairs) of the fly.

Research Products

• [Journal Article] (2012)
  • Author(s): You-Hong Jin, Motohide Takemura, Akira Furuyama, Norifumi Yonehara
  • Journal Title: Pain Research and Treatment Volume: vol.2012, Article ID 915706 Pages: 8-8
• [Journal Article] (2012)
  • Author(s): Adachi R, Sasaki Y, Morita H, Komai M, Shirakawa H, Goto T, Furuyama A, Isono K
  • Journal Title: J Neurogenet Volume: 26 Pages: 198-205
• [Journal Article] Peripheral Glutamate Receptors Are Required for Hyperalgesia Induced by Capsaicin (2011)
  • Author(s): You-Hong Jin, Motohide Takemura, Akira Furuyama and Norifumi Yonehara
  • Journal Title: Pain Research and Treatment Volume: 2012 Pages: 1-8
  • DOI: 10.1155/2012/915706
• [Presentation] (2013)
  • Author(s): 服部敬介、増澤徹、木村隆之、古山昭
  • Organizer: 日本機械学会 第25回バイオエンジニアリング講演会
  • Place of Presentation: つくば市
• [Presentation] 弾性ヒンジ型ナノ振動印加装置の研究開発 (2013)
  • Author(s): 服部敬介、増澤徹、木村隆之、古山昭
  • Organizer: 日本機械学会 第25回バイオエンジニアリング講演会
  • Place of Presentation: 茨城県つくば市 （独）産業技術総合研究所 つくばセンター
• [Presentation] (2012)
  • Author(s): 古山昭、大須賀謙二、米原典史、宗形芳英
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 郡山
• [Presentation] (2012)
  • Author(s): Akira furuyama, Norifumi Yonehara
  • Organizer: 日本比較生理生化学会第34回大会
  • Place of Presentation: 葉山
• [Presentation] The effect of capsaicin on the defensive reflexes for thermal and mechanical stimuli in frog (2012)
  • Author(s): 古山昭、米原典史
  • Organizer: 日本比較生理生化学会第34回大会
  • Place of Presentation: 神奈川県三浦郡葉山町 総合研究大学院大
• [Presentation] カプサイシンの皮下投与により生じるカエルの機械性アロディニア (2012)
  • Author(s): 古山昭、大須賀謙二、米原典史、宗形芳英
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 福島県郡山市 奥羽大学
• [Presentation] ショウジョウバエGr5a発現味細胞欠損が摂食行動に及ぼす影響 (2011)
  • Author(s): 古山昭
  • Organizer: 日本味と匂学会
  • Place of Presentation: 石川県金沢市
• [Book] Pain Research and Treatment (vol.2012, Article ID 915706) (2012)
  • Author(s): You-Hong Jin, Motohide Takemura, Akira Furuyama and Norifumi Yonehara
  • Total Pages: 8
• [Book] Pharmacology (2012)
  • Author(s): You-Hong Jin, Motohide Takemura, Akira Furuyama and Norifumi Yonehara
  • Publisher: INTECH