| Project/Area Number |
23659322
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Kansai Medical University |
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Principal Investigator |
ITO Seiji 関西医科大学, 医学部, 教授 (80201325)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Shinji 関西医科大学, 医学部, 講師 (70276393)
NISHIDA Kazuhiko 関西医科大学, 医学部, 助教 (80448026)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKATSUKA Terumasa 関西医療大学, 保健医療学部, 教授 (30380752)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 多光子励起顕微鏡 / 脊髄後角 / 一次求心性線維 / ゲートコントロール説 / in vivoイメージング / トランスジェニックマウス / 痛覚 / 神経回路網 / 脊髄 / 一時求心性線維 / in vivo イメージング / グルタミン酸受容体 / Ca応答 / レポーターマウス / 刺激応答 / 遺伝子改変マウス / 炎症モデル |
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| Research Abstract |
Peripheral stimuli in the skin are selected in the dorsal horn of spinal cord, and noxious stimuli are recognized as pain in the brain. In order to clarify the selection mechanism in the spinal cord, we established the imaging system for the spinal network which can chase for a long time in vivo using two-photon microscopy and gene-engineered mice expressing fluorescent protein. In this study, we demonstrated that intensity of neurites and puncta in the spinal cord increased for 30 min to 4 hours after injection of formalin in the foot, and that a network of neurons which respond to mechanical, touch, and thermal stimuli to the skin was visualized in vivo in fluorescent protein-expressing mice.
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