Development of gas phase sterilization technology for nucleolytic degradation
| Project/Area Number |
23659331
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
OKAZAKI Toshihiko 九州大学, 九州大学病院・ARO次世代医療センター, 特任准教授 (90529968)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 産業衛生 / 滅菌技術 / 核酸 / 気相滅菌機器 / 新規滅菌器機開発 / 核酸分解 |
|---|
| Research Abstract |
We have improved air intake and exhaust system of gas generating apparatus equipped with autocatalytic reaction components, resulting in capable of generating stable concentration of sterilization gas in a warehouse (also capable of monitoring gas concentration), and developing down-sizing system of apparatus, and also developing small chamber system for evaluating each experimental parameters. (Patent application number: 2012-062880, registered as a trademark as Biovector[〇!R]: reference number 12T13SX01) By use of this small chamber of sterilization system, we have evaluated the effect of sterilization of biological indicators and resolution of nucleic acid molecule s (DNA, RNA). By the sterilization gas exposure for 60 minutes under the temperature of 37 to 50 ℃, the complete eradication effect in all the objective bacilli applied in the amount of bacilli of 106has been achieved. Also in the evaluation of nucleolytic degradation ability using Bioanalyzer, the complete degradation ef
… More
fect in both of double strand DNA (dsDNA) and single strand DNA (ssDNA) into 10 or less base pairs was elucidated by the time of exposure for 30 minutes of sterilization gas under the condition of both of moist and dry. It also revealed that nucleolytic degradation ability of Biovector[〇!R] is temperature dependent (50>45>37 ℃). In the examination using the real time-PCR method, the complete degradation effect in both of RNA and ssDNA have been achieved by the time of exposure for 15 minutes of sterilization gas. In evaluation by the mycoplasma bacillus, certain validity also has been show, and still need to be elucidated. Taken together, in this study, newly developed gaseous sterilization system revealed its nucleolytic degradation ability, which could not be attained in the conventional method. For the next step, we endeavor to develop this innovative sterilization technology into the application to medical device by way of biosafety evaluation including corrosiveness and persistence as well as biological toxicity. Less
|
Report
(3 results)
Research Products
(16 results)
-
[Journal Article] Coxsackievirus B3 is an oncolyticvirus with immunostimulatory properties that is active against lung adenocarcinoma2012
Author(s)
Miyamoto S, Inoue H, Nakamura T, Yamada M, Sakamoto C, Urata Y, Okazaki T, Marumoto T, Takahashi A, Takayama K, Nakanishi Y, Shimizu H, Tani K
-
Journal Title
Cancer Res
Volume: 72(10)
Pages: 2609-21
Related Report
-
[Journal Article] Contribution of higher risk genes and European admixture to Crohn's disease in African Americans2012
Author(s)
Wang MH, OkazakiT, Kugathasan S, Cho JH, Isaacs KL, Lewis JD, Smoot DT, Valentine JF, Kader HA, Ford JG, Harris ML, Oliva-Hemker M, Cuffari C, Torbenson MS, Duerr RH, Silverberg MS, Rioux JD, Taylor KD, Nguyen GC, Wu Y, Datta LW, Hooker S, Dassopoulos T
-
Journal Title
Inflamm Bowel Dis
Volume: 18(12)
Pages: 2277-87
Related Report
-
-
[Journal Article] Development of novel immune therapies for solid tumors: phase I clinical trials in a single institute2012
Author(s)
HijikataY, Murahashi-Iga M, Okazaki T, Tanaka Y, Odaira K, Okano S, Hisano T, Takahashi A, Marumoto T, Inoue H, TaniK
-
Journal Title
Rinsho Ketsueki
Volume: 53(5)
Pages: 487-92
NAID
Related Report
-
[Journal Article] Coxsackievirus B3 Is an Oncolytic Virus with Immunostimulatory Properties that Is Active Against Lung Adenocarcinoma2012
Author(s)
Miyamoto S, Inoue H, Nakamura T, Yamada M, Sakamoto C, Urata Y, Okazaki T, Marumoto T, Takahashi A, Takayama K, Nakanishi Y, Shimizu H, Tani K
-
Journal Title
Cancer Research
Volume: (掲載確定)(印刷中)
Issue: 10
Pages: 2609-2621
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Coxsackievirus B3 Is an Oncolytic Virus with Immunostimulatory Properties that Is Active Against Lung Adenocarcinoma.2012
Author(s)
Miyamoto S, Inoue H, Nakamura T, Yamada M, Sakamoto C, Urata Y, Okazaki T, Marumoto T, Takahashi A, Takayama K, Nakanishi Y, Shimizu H, Tani K.
-
Journal Title
Cancer Research
Volume: march 29
Related Report
Peer Reviewed
-
-
-
-
-
-
-
