Development of assay system of biochemical markers by TaqMan protein quantification method useful for forensic diagnosis.
| Project/Area Number |
23659373
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Kurume University |
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Principal Investigator |
KODA Yoshiro 久留米大学, 医学部, 教授 (90231307)
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| Co-Investigator(Kenkyū-buntansha) |
SOEJIMA Mikiko 久留米大学, 医学部, 講師 (80279140)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 法医診断 / 蛋白定量 / 生化学マーカー / タンパク定量 / 生化学的マーカー / リアルタイムPCR / 抗原抗体反応 / 比較定量法 |
|---|
| Research Abstract |
Biochemical examinations useful for forensic diagnosis are usually performed by outsourcing. In this research, we examined usefulness of TaqMan protein assay to establish rapid and sample-saving on-site analysis method. As a result, we failed to design reproducible methods with wide dynamic range. On the other hand, we accomplish a part of the aim by using a commercially available testing equipment for private hospital.
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Report
(3 results)
Research Products
(33 results)
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[Journal Article] Nonsynonymous Single-Nucleotide Polymorphisms of the Human Apoptosis-Related Endonuclease - DNA Fragmentation Factor Beta Polypeptide, Endonuclease G, and Flap Endonuclease-1 - Genes Show a Low Degree of Genetic Heterogeneity.2012
Author(s)
Takeshita H, Fujihara J, Ueki M, Iida R,Koda Y, Soejima M, Yuasa I, Kato H, Nakajima T, Kominato Y, Yasuda T.
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Journal Title
DNA Cell Biol.
Volume: 1
Pages: 36-42
Related Report
Peer Reviewed
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[Journal Article] High prevalence of an anti-hypertriglyceridemic variant of the MLXIPL gene in Central Asia2011
Author(s)
Nakayama K, Yanagiswa Y, Ogawa A, Ishizuka Y, Munkhtulga L, Charupoonphol P, Supannnatas S, Kuartei S, Chimedregzen U, Koda Y, Ishida T, Kagawa Y, Iwamoto S
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Journal Title
J Hum Genet
Volume: 56
Issue: 12
Pages: 828-833
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Confirmation that SNPs in the high mobility group-A2 gene (HMGA2) are associated with adult height in the Japanese population ; wide-ranging population survey of height-related SNPs in HMGA22011
Author(s)
Takeshita H, Fujihara J, Soejima M, Koda Y, Kimura-Kataoka K, Ono R, Yuasa I, Iida R, Ueki M, Nagao M, Yasuda T
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Journal Title
Electrophoresis
Volume: 32
Issue: 14
Pages: 1844-1851
DOI
Related Report
Peer Reviewed
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[Journal Article] Functional and genetic survey of all known single-nucleotide polymorphisms within the human deoxyribonuclease I gene inwide-ranging ethnic groups.2011
Author(s)
Fujihara J, Ueki M, Yasuda T, Iida R, Soejima M, Koda Y, Kimura-Kataoka K, Kato H, Panduro A, Tongu M, Takeshita H.
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Journal Title
DNA Cell Biol.
Volume: 30
Pages: 205-217
Related Report
Peer Reviewed
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