Elucidation of cell supply system focusing on stem cells

• Project/Area Number: 23659384
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Sapporo Medical University
• Principal Investigator: SUZUKI syuuichirou 札幌医科大学, 医学部, 助教 (90532929)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 幹細胞 / 老年医学 / パーキンソン病 / 6-OHDA / 骨髄間葉系幹細胞 / ドーパミン神経 / ミクログリア / チロシンヒドロキシラーゼ

Research Abstract

We evaluated the multi-differentiation potential of mesenchymal stem cells from human peripheral blood (hPB-MSCs) to validate a hypothesis that the deteriorated supply system of innate stem cells induces neurodegenerative disease. Though the hypothesis was not completely endorsed, we continue to identify the optimal protocol of hPB-MSCs production. The therapeutic potential of MSCs from human bone marrow (hBM-MSCs) was evaluated in 6-OHDA induced Parkinson's disease(PD) model rats. Intravenous administration of hBM-MSCs inhibited drug-stimulated rotational behavior. Rats injected with hBM-MSCs displayed significant preservation of dopaminergic fibers in the striatum and dopaminergic neurons in the substantia nigra compared to those of control rats. The glial activity in the striatum was markedly inhibited by intravenous administration of hBM-MSCs. These findings raise the possibility that hBM-MSCs could be a novel therapeutic option to prevent PD development.

Research Products

• [Journal Article] 3-[(2,4-Dimethoxy)benzylidene]-anabasei nedihydrochloride protects against 6-hydroxydopamine-induced parkinsonian neurodegeneration through α7 nicotinic acetylcholine receptor stimulation in rats (2013)
  • Author(s): Suzuki S, Kawamata J, Matsushita T, Matsumura A, Hisahara S, Takata K, Kitamura Y, Kem W, Shimohama S
  • Journal Title: J Neurosci Res. Volume: 91(3) Issue: 3 Pages: 462-71
  • DOI: 10.1002/jnr.23160
  • Peer Reviewed
• [Presentation] パーキンソン病モデルラットに対する選択的α7 ニコチン性受容体作動薬による神経保護作用 (2013)
  • Author(s): 鈴木秀一郎,鈴木紘美,松村晃寛,久原真,川又純,下濱俊,高田和幸,北村佳久,谷口隆之
  • Organizer: 第54回日本神経学会学術大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2013-05-31
• [Presentation] パーキンソン病モデルラットに対する選択的ニコチン受容体作動薬による中脳黒質領域の免疫組織学的解析 (2012)
  • Author(s): 鈴木秀一郎,鈴木紘美,松村晃寛,松下隆司,久原真,川又純,下濱俊,高田和幸,北村佳久,谷口隆之
  • Organizer: 第53回日本神経学会学術大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2012-05-25
• [Presentation] パーキンソン病モデルラットにおけるヒト骨髄間葉系幹細胞の静脈投与の有効性について
  • Author(s): 鈴木秀一郎
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡国際センター