Functional roles of AF5q31and its relationship with anorexia nervosa.

• Project/Area Number: 23659385
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Wakayama Medical University
• Principal Investigator: MORIKAWA Yoshihiro 和歌山県立医科大学, 医学部, 准教授 (60230108)
• Co-Investigator(Renkei-kenkyūsha): KOMORI Tadasuke 和歌山県立医科大学, 医学部, 助教 (90433359) ; FURUTA Hiroto 和歌山県立医科大学, 医学部, 講師 (90238684)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ストレス / 絶食 / 転写因子 / モデルマウス / 神経性食思不振症 / 摂食調節 / 視床下部

Research Abstract

In a hypothalamic neuronal cell line, GT1-7 cells, AF5q31 specifically increased the expression of AMPKα2 subunit at the transcriptional level. In addition, AF5q31activated AMPK signal pathway by a novel mechanism, which is independent of AMPKαphosphorylation. AF5q31+/-mice exhibited the disturbance of both HPA axis and glucose metabolism, which are observed in the patients with anorexia nervosa. Thus, AF5q31+/-mice provide a unique mouse model of anorexia nervosa.

Research Products

• [Journal Article] Regulation of AMP-activated Protein Kinase Signaling by AFF4, a Member of the AF4 (ALL1-fused Gene from Chromosome 4) Family of Transcription Factors, in Hypothalamic Neurons (2012)
  • Author(s): Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y.
  • Journal Title: J Biol Chem Volume: 287 Issue: 24 Pages: 19985-19996
  • DOI: 10.1074/jbc.m112.367854
  • Peer Reviewed
• [Presentation] 視 床 下 部神経細胞でのAMPKシグナル活性化における転写因子AFF4の役割 (2012)
  • Author(s): 小森忠祐、土 井 麻 子 、古田浩人、赤 水 尚 史 、森川吉博
  • Organizer: 第24回分子糖尿病学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-12-08
• [Presentation] Regulation of AMP-activated protein kinase alpha 2 expression by a fasting/ghrelin-induced transcription factor, AF5q31, in the hypothalamic neurons (2012)
  • Author(s): Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y
  • Organizer: The 48th EASD Annual Meeting 2012.10.2
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2012-10-02
• [Presentation] 絶食により視床下部において誘導される新規転写因子の検討 (2012)
  • Author(s): 小森忠祐, 仙波恵美子, 森川吉博
  • Organizer: 第80回和歌山医学会総会
  • Place of Presentation: 和歌山
  • Year and Date: 2012-07-08
• [Presentation] 視床下部神経細胞でのAMPKシグナル活性化における転写因子AFF4の役割
  • Author(s): 小森忠祐
  • Organizer: 第２４回分子糖尿病学シンポジウム
  • Place of Presentation: 東京
• [Presentation] Regulation of AMP-activated protein kinase alpha 2 expression by a fasting/ghrelin-induced transcription factor, AF5q31, in the hypothalamic neurons
  • Author(s): Komori T
  • Organizer: 48th Annual Meeting of the European Association for the Study of Diabetes (EASD)
  • Place of Presentation: Berlin, Germany
• [Presentation] 絶食により視床下部において誘導される新規転写因子の検討
  • Author(s): 小森忠祐
  • Organizer: 第８０回和歌山医学会総会
  • Place of Presentation: 和歌山