Development of a novel drug-delivery system by a bio-capsule that is built by primary intestinal epithelial cells.
| Project/Area Number |
23659394
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
WATANABE Mamoru 東京医科歯科大学, 医歯学総合研究科, 教授 (10175127)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAMURA Tetsuya 東京医科歯科大学, 医歯学総合研究科, 准教授 (70265809)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 腸管上皮 / バイオカプセル / 薬剤デリバリー / 炎症性腸疾患 / 初代培養法 |
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| Research Abstract |
Through our studies aimed to establish novel methods to generate “bio-capsules” that are built by intestinal epithelial cells, we succeeded to identify a distinct culture condition, which allows us to produce “bio-capsules” in a highly efficient manner. In addition, we found that the capsules that were generated by the present method are consisted with high number of intestinal stem cells. Also, we confirmed that Rhodamine123, a substrate of P-glycoprotein, could easily accumulate within the inner space of the bio-capsule. These findings establish the basis for the efficient generation of drug-containing, intestinal epithelial cell-derived bio-capsules.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+stem cell2012
Author(s)
Yui S, Nakamura T, Sato T, Nemoto Y, Mizutani T, Zheng X, Ichinose S, Nagaishi T, Okamoto R, Tsuchiya K, Clevers H, and Watanabe M
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Journal Title
Nature Medicine
Volume: 18
Issue: 4
Pages: 618-623
DOI
Related Report
Peer Reviewed
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[Presentation] Regeneration of damaged colon epithelium by transplanted colon Lgr5+ stem cells maintained and expanded in vitro2012
Author(s)
Yui S, Nakamura T, Nemoto Y, Mizutani T, Fukuda M, Nozaki K, Yamauchi Y, Mochiduki W, Zheng X, Nagaishi T, Okamoto R, Tsuchiya K, Watanabe M
Organizer
第10回幹細胞シンポジウム
Place of Presentation
淡路島
Year and Date
2012-06-01
Related Report
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[Presentation] Real-time analysis of p-glycoprotein-mediated drug transport across primary intestinal epitherial cells three-dimensionallycultured in vitro2012
Author(s)
Mizutani T, Nakamura T, Morikawa R, Fukuda M, Mochizuki W, Yamauchi Y,Nozaki K, Yui S, Okamoto R, Tsuchiya K, Watanabe M
Organizer
DDW2012
Place of Presentation
San Diego, U.S.A
Year and Date
2012-05-21
Related Report
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