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Epigenomic analysis of gastrointestinal cancer stroma

Research Project

Project/Area Number 23659400
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionSapporo Medical University

Principal Investigator

SHINOMURA Yasuhisa  札幌医科大学, 医学部, 教授 (90162619)

Co-Investigator(Renkei-kenkyūsha) SUZUKI Hiromu  札幌医科大学, 医学部, 助教 (20381254)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords胃癌 / 癌間質 / エピジェネティクス / メチル化 / 癌幹細胞
Research Abstract

We aimed to analyze epigenome alterations in gastrointestinal cancer cells,cancerstroma and background non-cancerous mucosa. By performing Methylated CpG Amplification (MCA)-microarray analysis and bisulfite-pyrosequencing analysis, we detected methylationof tumor-related genes, such as SFRP1 and miR-34b/c, in gastric cancer cells as well as cancer stroma. Moreover, we identified aberrant methylation of a candidate cancer-related gene, RASGRF1, in both gastric cancer cells and background non-cancerous mucosa. We also found that methylationof RASGRF1 could be a potential biomarker to assess gastric cancer risk.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2013 2012 2011

All Journal Article (24 results) (of which Peer Reviewed: 24 results) Presentation (13 results)

  • [Journal Article] Cancer detection by ubiquitin carboxyl-terminal esterase L1 methylation in pancreatobiliary fluids2013

    • Author(s)
      Kato N, Yamamoto H, Adachi Y, Ohashi H, Taniguchi H, Suzuki H, Nakazawa M, Kaneto H, Sasaki S, Imai K, Shinomura Y
    • Journal Title

      World J Gastroenterol

      Volume: 19(11) Issue: 11 Pages: 1718-1727

    • DOI

      10.3748/wjg.v19.i11.1718

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alterations in the human epidermal growth factor receptor 2-phosphatidylinositol 3-kinase-v-Akt pathway in gastric cancer.2012

    • Author(s)
      Sukawa Y, Yamamoto H, Nosho K, Kunimoto H, Suzuki H, Adachi Y, Nakazawa M, Nobuoka T, Kawayama M,Mikami M, Matsuno T, Hasegawa T, Hirata K, Imai K, Shinomura Y
    • Journal Title

      World J Gastroenterol

      Volume: 18 Pages: 6577-6586

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular dissection of premalignant colorectal lesions reveals early onset of the CpG island methylator phenotype2012

    • Author(s)
      Yamamoto E, Suzuki H, Yamano HO, Maruyama R, Nojima M, Kamimae S, Sawada T, Ashida M, Yoshikawa K, Kimura T, Takagi R, Harada T, Suzuki R, Sato A, Kai M, Sasaki Y, Tokino T, Sugai T, Imai K, Shinomura Y, Toyota M
    • Journal Title

      Am J Pathol

      Volume: 181 Pages: 1847-1861

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant methylation of RASGRF1 is associated with an epigenetic field defect and increased risk of gastric cancer2012

    • Author(s)
      Takamaru H, Yamamoto E, Suzuki H, Nojima M, Maruyama R, Yamano HO, Yoshikawa K, Kimura T, Harada T, Ashida M, Suzuki R, Yamamoto H, Kai M, Tokino T, Sugai T, Imai K, Toyota M, Shinomura Y.
    • Journal Title

      Cancer Prev Res

      Volume: 5 Pages: 1203-1212

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Model of translational cancer research in multiple myeloma2012

    • Author(s)
      Yasui H, Ishida T, Maruyama R, Nojima M, Ikeda H, Suzuki H, Hayashi T, Shinomura Y, Imai K
    • Journal Title

      Cancer Sci

      Volume: 103 Pages: 1907-1912

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interrelationship between microsatellite instability and microRNA in gastrointestinal cancer2012

    • Author(s)
      Yamamoto H, Adachi Y, Taniguchi H, Kunimoto H, Nosho K, Suzuki H, Shinomura Y
    • Journal Title

      World J Gastroenterol

      Volume: 18 Pages: 2745-2755

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide analysis of DNA methylation identifies novel cancer-related genes in hepatocellular carcinoma2012

    • Author(s)
      Shitani M, Sasaki S, Akutsu N, Takagi H, Suzuki H, Nojima M, Yamamoto H, Tokino T, Hirata K, Imai K, Toyota M, Shinomura Y
    • Journal Title

      Tumour Biol

      Volume: 33 Pages: 1307-1317

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors2012

    • Author(s)
      Niinuma T, Suzuki H, Nojima M, Nosho K, Yamamoto H, Takamaru H, Yamamoto E, Maruyama R, Nobuoka T, Miyazaki Y, Nishida T, Bamba T, Kanda T, Ajioka Y, Taguchi T, Okahara S, Takahashi H, Nishida Y, Hosokawa M, Hasegawa T, Tokino T, Hirata K, Imai K, Toyota M, Shinomura Y
    • Journal Title

      Cancer Res

      Volume: 72 Pages: 1126-1136

    • NAID

      10031131121

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Emerging links between epigenetic alterations and dysregulation of noncoding RNAs in cancer2012

    • Author(s)
      Maruyama R, Suzuki H, Yamamoto E, Imai K, Shinomura Y
    • Journal Title

      Tumour Biol

      Volume: 33 Pages: 277-85

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel pit pattern identifies the precursor of colorectal cancer derived from sessile serrated adenoma2012

    • Author(s)
      Kimura T, Yamamoto E, Yamano HO, Suzuki H, Kamimae S, Nojima M, SawadaT, Ashida M, Yoshikawa K, Takagi R, Kato R, Harada T, Suzuki R, Maruyama R, Kai M, Imai K, Shinomura Y, Sugai T, Toyota M
    • Journal Title

      Am J Gastroenterol

      Volume: 107 Pages: 460-469

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] IGF-I receptor expression is associated with aggressive phenotypes and has therapeutic activity in biliary tract cancers2012

    • Author(s)
      Ohashi H, Adachi Y, Yamamoto H, Taniguchi H, Nosho K, Suzuki H, Arimura Y, Imai K, Carbone DP, Shinomura Y
    • Journal Title

      Cancer Sci

      Volume: 103 Pages: 252-261

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant DNA methylation associatedwith aggressiveness of gastrointestinal stromal tumour2012

    • Author(s)
      Okamoto Y, Sawaki A, Ito S, Nishida T, Takahashi T, Toyota M, Suzuki H, Shinomura Y, Takeuchi I, Shinjo K, An B, Ito H, Yamao K, Fujii M, Murakami H, Osada H, Kataoka H, Joh T, Sekido Y, Kondo Y
    • Journal Title

      Gut

      Volume: 61 Pages: 392-401

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant Methylation of RASGRF1 Is Associated with an Epigenetic Field Defect and Increased Risk of Gastric Cancer.2012

    • Author(s)
      Takamaru H, Yamamoto E, Suzuki H.
    • Journal Title

      Cancer Prev Res (Phila)

      Volume: 5(10) Issue: 10 Pages: 2012-2012

    • DOI

      10.1158/1940-6207.capr-12-0056

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alterations in the human epidermal growth factor receptor 2-phosphatidylinositol 3-kinase-v-Akt pathway in gastric cancer2012

    • Author(s)
      Sukawa Y, Yamamoto H (他11名), Nosho K, Shinomura Y
    • Journal Title

      World J Gastroenterol

      Volume: 18 Issue: 45 Pages: 6577-6586

    • DOI

      10.3748/wjg.v18.i45.6577

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant DNA methylation associated with aggressiveness of gastrointestinal stromal tumour2012

    • Author(s)
      Okamoto Y
    • Journal Title

      Gut

      Volume: 61 Issue: 3 Pages: 392-401

    • DOI

      10.1136/gut.2011.241034

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Upregulation of miR-196a and HOTAIR drive malignant character in gastrointestinal stromal tumors2012

    • Author(s)
      Takeshi Niinuma
    • Journal Title

      Cancer research

      Volume: 72 Issue: 5 Pages: 1126-36

    • DOI

      10.1158/0008-5472.can-11-1803

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The efficacy of IGF-I receptor monoclonal antibody against human gastrointestinal carcinomas is independent of k-ras mutation status2011

    • Author(s)
      Ii M, Li H, Adachi Y, Yamamoto H, Ohashi H, Taniguchi H, Arimura Y, Carbone DP, Imai K, Shinomura Y
    • Journal Title

      Clin Cancer Res

      Volume: 17 Pages: 5048-5059

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Insulin-like growth factor-I receptor blockade reduces tumor angiogenesis and enhances the effects of bevacizumab for a human gastric cancer cell line, MKN452011

    • Author(s)
      Li H, Adachi Y, Yamamoto H, Min Y, Ohashi H, Ii M, Arimura Y, Endo T, Lee CT, Carbone DP, Imai K, Shinomura Y
    • Journal Title

      Cancer

      Volume: 117 Pages: 3135-3147

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide profiling of chromatin signatures reveals epigenetic regulation of microRNA genes in colorectal cancer2011

    • Author(s)
      Suzuki H, Takatsuka S, Akashi H, Yamamoto E, Nojima M, Maruyama R, Kai M, Yamano HO, Sasaki Y, Tokino T, Shinomura Y, Imai K, Toyota M
    • Journal Title

      Cancer Res

      Volume: 71 Pages: 5646-5658

    • NAID

      130004436468

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Co-expression of laminin β3 and γ2 chains and epigenetic inactivation of laminin α3 chain in gastric cancer2011

    • Author(s)
      Ii M, Yamamoto H, Taniguchi H, Adachi Y, Nakazawa M, Ohashi H, Tanuma T, Sukawa Y, Suzuki H, Sasaki S, Imai K, Shinomura Y
    • Journal Title

      Int J Oncol

      Volume: 39 Pages: 593-599

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interferon-α/β and anti-fibroblast growth factor receptor 1 monoclonal antibody suppress hepatic cancer cells in vitro and in vivo2011

    • Author(s)
      Sasaki S, Ishida T, Toyota M, Ota A, Suzuki H, Takaoka A, Yasui H, Yamamoto H, Takagi H, Maeda M, Seito T, Tsujisaki M, Shinomura Y, Imai K
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic alteration of DNA in mucosal wash fluid predicts invasiveness of colorectal tumors2011

    • Author(s)
      Kamimae S, Yamamoto E, Yamano HO, Nojima M, Suzuki H, Ashida M, Hatahira T, Sato A, Kimura T, Yoshikawa K, Harada T, Hayashi S, Takamaru H, Maruyama R, Kai M, Nishiwaki M, Sugai T, Sasaki Y, Tokino T, Shinomura Y, Imai K, Toyota M
    • Journal Title

      Cancer Prev Res

      Volume: 4 Pages: 674-683

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis2011

    • Author(s)
      Hizaki K, Yamamoto H, Taniguchi H, Adachi Y, Nakazawa M, Tanuma T, Kato N, Sukawa Y, Sanchez JV, Suzuki H, Sasaki S, Imai K, Shinomura Y
    • Journal Title

      Mod Pathol

      Volume: 24 Pages: 876-884

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic inactivation of calcium-sensing receptor in colorectal carcinogenesis2011

    • Author(s)
      Hizaki K, Yamamoto H, Taniguchi H, Adachi Y, Nakazawa M, Tanuma T, Kato N, Sukawa Y, Sanchez JV, Suzuki H, Sasaki S, Imai K, Shinomura Y
    • Journal Title

      Mod Pathol

      Volume: 24 Issue: 6 Pages: 876-884

    • DOI

      10.1038/modpathol.2011.10

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 篠村恭久.miR-34b/cの異常メチル化は胃癌の異時性多発リスクに関与する2012

    • Author(s)
      鈴木亮,山本英一郎,鈴木拓,丸山玲緒,能正勝彦,山本博幸,野島正寛,甲斐正広,山野泰穂,菅井有,今井浩三
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] microRNA-34b/cの異常メチル化は胃癌の異時性多発リスクに関与する2012

    • Author(s)
      鈴木亮,山本英一郎,鈴木拓,野島正寛,丸山玲緒,能正勝彦,山本博幸,甲斐正広,山野泰穂,菅井有,今井浩三,篠村恭久
    • Organizer
      第32回日本分子腫瘍マーカー研究会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] GISTの悪性度マーカーおよび治療標的としてのnon-coding RNA2012

    • Author(s)
      新沼猛,鈴木拓,野島正寛,高丸博之,山本英一郎,能正勝彦,山本博幸,篠村恭久
    • Organizer
      第8回日本消化管学会総会学術集会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report
  • [Presentation] 胃癌におけるPI3K-Akt経路に関わるHER2発現、PIK3CA変異、EBV感染の臨床病理および分子学的意義に関する検討2012

    • Author(s)
      須河恭敬
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] miR-34b/cの異常メチル化は胃癌の異時性多発リスクに関与する2012

    • Author(s)
      鈴木 亮
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] 胃癌におけるPI3K-Akt経路下流シグナル異常活性化に関わる因子に関する検討2012

    • Author(s)
      須河恭敬
    • Organizer
      第20回日本消化器関連学会週間(JDDW)
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Presentation] 胃癌におけるエピジェネティックな遺伝子異常の網羅的解析2012

    • Author(s)
      高丸博之、鈴木拓、山本英一郎、神前正幸、新沼猛、丸山玲緒、豊田実、篠村恭久
    • Organizer
      第8回日本消化管学会総会学術集会
    • Place of Presentation
      仙台
    • Related Report
      2011 Research-status Report
  • [Presentation] 消化管間質腫瘍におけるmicroRNA発現プロファイル解析2011

    • Author(s)
      新沼猛,鈴木拓,野島正寛,高丸博之,能正勝彦,山本博幸,豊田実,篠村恭久
    • Organizer
      第53回日本消化器病学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] DNAメチル化異常の網羅的な解析による新規胃癌関連遺伝子の検索2011

    • Author(s)
      高丸博之,鈴木拓,山本英一郎,新沼猛,山本博幸,有村佳昭,豊田実,篠村恭久
    • Organizer
      第53回日本消化器病学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 胃癌におけるゲノムワイドな低メチル化と染色体不安定性2011

    • Author(s)
      鈴木亮,山本英一郎,鈴木拓,丸山玲緒,山野泰穂,今井浩三,豊田実,篠村恭久
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] 胃癌におけるエピジェジェティックな遺伝子異常の網羅的解析2011

    • Author(s)
      高丸博之,鈴木拓,山本英一郎,丸山玲緒,神前正幸,新沼猛,青木由佳,澤田武,豊田実,篠村恭久
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] 胃癌におけるエピジェネティックな遺伝子異常の網羅的解析2011

    • Author(s)
      高丸博之、鈴木拓、山本英一郎、丸山玲緒、神前正幸、新沼猛、青木由佳、澤田武、豊田実、篠村恭久
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Presentation] 消化管間質腫瘍におけるmicroRNA発現プロファイル解析2011

    • Author(s)
      新沼猛、鈴木 拓、野島正寛、高丸博之、能正勝彦、山本博幸、豊田実、篠村恭久
    • Organizer
      第19回日本消化器関連学会週間
    • Place of Presentation
      福岡
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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