Role and function of taxilin in the complex formation of molecules related to intracellular signal transduction in a regenerating liver

• Project/Area Number: 23659403
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Dokkyo Medical University
• Principal Investigator: SHIRATAKI Hiromichi 獨協医科大学, 医学部, 教授 (90249962)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: タキシリン / 細胞内小胞輸送 / トランスフェリン / ソーティングネキシン / 微小管 / 再生肝 / 小胞輸送 / トランスフェリン受容体 / 再生 / 細胞・組織 / シグナル伝達 / 再生医学

Research Abstract

In this project, we attempted to reveal the mechanism by which -taxilin, a molecule related to vesicle transport, regulates intracellular delivery of molecules related to intracellular signal transduction. We obtained the following results: 1) -Taxilin was overexpressed in the regenerating liver after partial hepatectomy. 2) -Taxilin was localized on some intracellular organelles connected to the microtubule in Hela cells. 3) -Taxilin interacted with sorting nexin 4 and was implicated in the recycling of transferrin receptor. On the basis of these results, the possibility was raised that -taxilin might be involved in the complex formation of molecules related to intracellular signal transduction that are connected with transferrin receptor through regulating intracellular vesicle transport.

Research Products

• [Journal Article] α-Taxilin interacts with soring nexin 4 and participates in the recycling pathway of transferrin receptor. (2014)
  • Author(s): Sakane, H., Horii, Y., Nogami, S., Kawano, Y., Kaneko-Kawano, T., and Shirataki, H.
  • Journal Title: PloS ONE Volume: 9 Issue: 4 Pages: e93509-e93509
  • DOI: 10.1371/journal.pone.0093509
  • Peer Reviewed / Open Access
• [Journal Article] Expression of α-taxilin in the murine gastrointestinal tract: potential implication in cell proliferation. (2014)
  • Author(s): Horii Y, Sakane H, Nogami S, Ohtomo N, Tomiya T, Shirataki H.
  • Journal Title: Histochem Cell Biol. Volume: 141 Issue: 2 Pages: 165-80
  • DOI: 10.1007/s00418-013-1147-0
  • Peer Reviewed
• [Journal Article] Interaction ofα-taxilin localized on intracellular components with the microtubule cytoskeleton (2012)
  • Author(s): Horii Y.、Nogami S.、Kawano Y.、Kaneko-Kawano K.、Ohtomo N.、Tomiya T.、and Shirataki H.
  • Journal Title: Cell Struc. Func Volume: 37巻 Pages: 111-126
  • URL: https://www.jstage.jst.go.jp/browse/csf
  • Peer Reviewed
• [Journal Article] Interaction of α-taxilin localized on intracellular components with the microtubule cytoskeleton. (2012)
  • Author(s): Horii, Y., Nogami, S., Kawano, Y., Kaneko-Kawano, K., Ohtomo, N., Tomiya, T., and Shirataki, H.
  • Journal Title: Cell Struc. Func. Volume: 37 Pages: 111-126
  • NAID: 130004137583
  • Peer Reviewed
• [Journal Article] Serum interferon alpha receptor2 mRNA may predict efficacy of interferon alpha with/without low-dose sorafenib for matastatic clear renal cell carcinoma (2011)
  • Author(s): Furuya N.、Kamai T.、Shirataki H.、Yanai Y.、Fukuda T.、Nakamura F.、Kambara T.、Nakanishi K.、Abe H.、and Yoshida K.
  • Journal Title: Cancer Immunol. Immunother Volume: 60巻 Issue: 6 Pages: 793-808
  • DOI: 10.1007/s00262-011-0989-3
  • Peer Reviewed
• [Journal Article] Overexpression ofα -taxilin is associated with the metastatic potential in renal cell cancer (2011)
  • Author(s): Mashidori T.、Shirataki H.、Kamai T.、Nakamura F.、Abe H.、Honda M.、Yamanishi T.、and Yoshida K.
  • Journal Title: Biolmed. Res Volume: 32巻 Pages: 103-110
  • URL: https://www.jstage.jst.go.jp/browse/biomedres
  • Peer Reviewed
• [Journal Article] Increased alpha-taxilin protein expression is associated with the metastatic and invasive potential of renal cell cancer (2011)
  • Author(s): Mashidori, T., Shirataki, H. , Kamai, T., Nakamura, F.,Abe, H., Honda, M., Yamanishi, T., and Yoshida, K.
  • Journal Title: Biomedical Research Volume: 32 Issue: 2 Pages: 103-110
  • DOI: 10.2220/biomedres.32.103
  • NAID: 130004470769
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Expression of alpha-taxilin correlates with growth activity and malignant potential of hepatocellular carcinoma. (2011)
  • Author(s): Ohtomo, N., Shirataki, H., and Tomiya, T.
  • Journal Title: Int. J. Mol. Med. Volume: 28
• [Presentation] IGF-Ireduces collagen production through induction of collagen degradation via mTOR dependent signaling pathway (2013)
  • Author(s): 西川尚子、富谷智明、大友夏子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: 第20回肝細胞研究会
  • Place of Presentation: 大阪
• [Presentation] 肝星細胞コラーゲン産生に対するIGF-I の調節作用およびその機序の検討 (2013)
  • Author(s): 西川尚子、富谷智明、大友夏子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: JDDW2013(第21回日本消化器関連学会週間、第17回日本肝臓学会大会)
  • Place of Presentation: 東京
• [Presentation] IGF-I reduces collagen production through induction of collagen degradation via mTOR dependent signaling pathway (2013)
  • Author(s): 西川尚子、富谷智明、大友夏子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: 第20回肝細胞研究会
  • Place of Presentation: 大阪
• [Presentation] 肝星細胞コラーゲン産生に対するIGF-Iの調節作用およびその機序の検討 (2013)
  • Author(s): 西川尚子、富谷智明、大友夏子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: JDDW2013(第21回日本消化器関連学会週間、第17回日本肝臓学会大会
  • Place of Presentation: 東京
• [Presentation] 肝星細胞におけるコラーゲン産生に対するIGF-I の直接作用 (2012)
  • Author(s): 西川尚子、富谷智明、大友夏子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: 第48回日本肝臓学会総会、オープンワークショップ38「肝線維化」
  • Place of Presentation: 金沢
• [Presentation] 肝星細胞の増殖とコラーゲン産生に対するIGF-I の影響に関する検討 (2012)
  • Author(s): 西川尚子、富谷智明、大友夏子、西川尚子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: 第19回肝細胞研究会
  • Place of Presentation: 札幌
• [Presentation] Insulin-like growth factor I reduces collagen production by hepatic stellate cells even when their proliferative activity is stimulated (2012)
  • Author(s): Takako Nishikawa、Tomoaki Tomiya、Natsuko Ohtomo、Yukiko Inoue、Hitoshi Ikeda、Hiromichi Shirataki、and Kzuhiro Koike
  • Organizer: Asian Pacific Association for the study of the liver
  • Place of Presentation: Taipei
• [Presentation] Reduction of collagen production by insulin-like growth factor I is independent of modulation of activation state in hepatic stellate cells (2012)
  • Author(s): Takako Nishikawa、Tomoaki Tomiya、Natsuko Ohtomo、Yukiko Inoue、Hitoshi Ikeda、Hiromichi Shirataki、and Kazuhiko Koike
  • Organizer: The 61th annual meeting of the American association for the study for the liver
  • Place of Presentation: Boston
• [Presentation] Reduction of collagen production by insulin-like growth factor I is independent of modulation of activation state in hepatic stellate cells. (2012)
  • Author(s): Takako Nishikawa, Tomoaki Tomiya, Natsuko Ohtomo, Yukiko Inoue, Hitoshi Ikeda, Hiromichi Shirataki, and Kazuhiko Koike
  • Organizer: The 61th annual meeting of the American association for the study for the liver
  • Place of Presentation: Boston
• [Presentation] 肝星細胞におけるコラーゲン産生に対するIGF-Iの直接作用 (2012)
  • Author(s): 西川尚子、富谷智明、大友夏子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: 第4８回日本肝臓学会総会
  • Place of Presentation: 金沢
• [Presentation] 肝星細胞の増殖とコラーゲン産生に対するIGF-Iの影響に関する検討 (2012)
  • Author(s): 西川尚子、富谷智明、大友夏子、西川尚子、井上有希子、池田均、白瀧博通、小池和彦
  • Organizer: 第19回肝細胞研究会
  • Place of Presentation: 札幌
• [Presentation] Insulin-Like Growth Factor I Stimulates Proliferation and Protein Production in Rat Hepatocytes through an mTOR Signaling Pathway. (2011)
  • Author(s): Tanoue, Y., Tomiya, T., Ohtomo, N., Inoue, Y., Nishikawa,T., Ikeda, H., Shirataki, H., Kzuhiro, K., and Fujiwara, K.
  • Organizer: The 46th annual meeting of the European association for the study for the liver
  • Place of Presentation: Berllin
• [Presentation] Expression of alpha-taxilin correlates with growth activity and malignant potential of hepatocellular carcinoma. (2011)
  • Author(s): Ohtomo, N., Shirataki, H., and Tomiya, T.
  • Organizer: The 16th world congress on advances in oncology and the 14th international symposium on molecular medicine(招待講演)
  • Place of Presentation: Rhodos
• [Remarks]
  • URL: http://www.dokkyomed.ac.jp/dep-m/mol-cell-bio/index.html
• [Remarks] 獨協医科大学 分子細胞生物学講座
  • URL: http://www.dokkyomed.ac.jp/dep-m/mol-cell-bio/index.html