| Project/Area Number |
23659405
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
KOJIMA Masayasu 久留米大学, 分子生命科学研究所, 教授 (20202062)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 下部消化管学(小腸、大腸)、 / 消化管幹細胞 / GPCR / オーファン受容体 / LGR5 / リガンド探索 |
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| Research Abstract |
An orphan GPCR LGR5 receptor is a marker for gastrointestinal stem cells. However,the endogenous ligand for LGR5 has not been discovered. We established a cell line, whichstably expressed LGR5, and used it for a systematic assay on brain and gastrointestinaltissue peptide extracts to search for the ligands. We detected a positive signal in thebrain samples and purified it to a single peptide. By amino acid sequence analysis, wefound that the purified peptide is CGRP, a neuropeptide. We thought that CGRP should havereacted to endogenous CGRP receptors on CHO cells. The real endogenous ligands for LGR5and its relative receptors, LGR4 and LGR6, are still controversial.
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