Search for novel peptide hormones, which act on gastrointestinal stemcells, and its role on the regeneration of gastrointestinal epithelium
| Project/Area Number |
23659405
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Kurume University |
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Principal Investigator |
KOJIMA Masayasu 久留米大学, 分子生命科学研究所, 教授 (20202062)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 下部消化管学(小腸、大腸)、 / 消化管幹細胞 / GPCR / オーファン受容体 / LGR5 / リガンド探索 |
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| Research Abstract |
An orphan GPCR LGR5 receptor is a marker for gastrointestinal stem cells. However,the endogenous ligand for LGR5 has not been discovered. We established a cell line, whichstably expressed LGR5, and used it for a systematic assay on brain and gastrointestinaltissue peptide extracts to search for the ligands. We detected a positive signal in thebrain samples and purified it to a single peptide. By amino acid sequence analysis, wefound that the purified peptide is CGRP, a neuropeptide. We thought that CGRP should havereacted to endogenous CGRP receptors on CHO cells. The real endogenous ligands for LGR5and its relative receptors, LGR4 and LGR6, are still controversial.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Isolation of the bioactive peptides CCHamide-1 and CCHamide-2 from Drosophila and their putative role in appetite regulation as ligands for G protein-coupled receptors2012
Author(s)
Ida T, Takahashi T, Tominaga H, Sato T, Sano H, Kume K, Ozaki M, Hiraguchi T, Shiotani H, Terajima S, Nakamura Y, Mori K, Yoshida M, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M
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Journal Title
Front Endocrinol (Lausanne)
Volume: 3
Pages: 177-177
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of the endogenous cysteine-rich peptide trissin, ligand for an orphan G-protein-coupled receptor in Drosophila2011
Author(s)
Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Yoshizawa-Kumagae K, Nishio H, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M
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Journal Title
Biochem Biophys Res Commun
Volume: 414
Issue: 1
Pages: 44-48
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila2011
Author(s)
Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozak Mi, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K, Kojima M
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Journal Title
Biochem Biophys Res Commun
Volume: 410
Issue: 4
Pages: 872-877
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of the endogenous cysteine-rich peptide trissin, a ligand for an orphan G protein-coupled receptor in Drosophila2011
Author(s)
Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Yoshizawa-Kumagaye K, Nishio H, Kato J, Murakami N, Miyazato M, Kangawa K, Kojima M
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Journal Title
Biochem Biophys Res Commun
Volume: 414
Pages: 44-48
Related Report
Peer Reviewed
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[Journal Article] Identification of the novel bioactive peptides dRYamide-1 and dRYamide-2, ligands for a neuropeptide Y-like receptor in Drosophila2011
Author(s)
Ida T, Takahashi T, Tominaga H, Sato T, Kume K, Ozaki M, Hiraguchi T, Maeda T, Shiotani H, Terajima S, Sano H, Mori K, Yoshida M, Miyazato M, Kato J, Murakami N, Kangawa K, Kojima M
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Journal Title
Biochem Biophys Res Commun
Volume: 410
Pages: 872-877
Related Report
Peer Reviewed
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