Analysis of hepatitis C virus sequence quasi species by NGS
| Project/Area Number |
23659407
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
WAKITA Takaji 国立感染症研究所, ウイルス第二部, 部長 (40280789)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 肝炎ウイルス / 次世代シーケンス / quasispecies / HCV / ウイルス / インターフェロン |
|---|
| Research Abstract |
Hepatitis C Virus (HCV) genome accumulates nucleotide substitutions because of low viral RNA polymerase fidelity and high viral replication ratio. This quasispecies nature has been considered to contribute for its pathogenesis or viral escape. We constructed a method to determine the full-length viral genome RNA species from patient serum combined next-generation sequencing and capillary sequencing. We found independent dominant species co-existed in one patient serum. Polymorphisms related to the favorable response of interferon sensitivity were gathered into one species. Sequential species were formed same clusters. We thus considered that both species might have been mixture and survived the bottleneck events when the patient was infected, and then evolved individually in the patient. In conclusion, our study demonstrated that considering each full-length species in patient serum is important for understanding HCV life cycle.
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Report
(4 results)
Research Products
(80 results)
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[Journal Article] Neutralizing antibodies induced by cell culture-derived hepatitis C virus protect against infection in mice.2013
Author(s)
Akazawa D, Moriyama M, Yokokawa H, Omi N, Watanabe N, Date T, Morikawa K, Aizaki H, Ishii K, Kato T, Mochizuki H, Nakamura N, Wakita T.
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Journal Title
Gastroenterology
Volume: 145
Issue: 2
Pages: 447-455
DOI
Related Report
Peer Reviewed
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[Journal Article] In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis2011
Author(s)
Saeed M, Shiina M, Date T, Akazawa D, Watanabe N, Murayama A, Suzuki T, Watanabe H, Hiraga N, Imamura M, Chayama K, Choi Y, Krawczynski K, Liang TJ, Wakita T, Kato T
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Journal Title
Hepatology
Volume: 54
Issue: 2
Pages: 425-433
DOI
Related Report
Peer Reviewed
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[Presentation] Identification of novel NS5A-associated proteins in the host-cell membrane fraction and their role in HCV life cycle2011
Author(s)
K Goto, T Kimura, K Watashi, R Suzuki, S Yamagoe, T Miyamura, K Moriya, H Yotsuyanagi, K Koike, T Suzuki, T Wakita, H Aizaki
Organizer
18th International Meeting on Hepatitis C and Related Viruses
Place of Presentation
Seattle Sheraton Hotel, Seattle, USA
Related Report
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[Presentation] Isolation of a highly infectious hepatitis C virus with adaptive mutations2011
Author(s)
M Fukasawa, Y Shirasago, K Saito, Y Murakami, H Fukazawa, T Suzuki, R Suzuki, T Wakita, K Hanada, J Chiba
Organizer
18th International Meeting on Hepatitis C and Related Viruses
Place of Presentation
Seattle Sheraton Hotel, Seattle, USA
Related Report
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[Presentation] Immunological memory response to induce neutralizing immunoglobulin in HCV particles-immunized mice2011
Author(s)
M Moriyama, D Akazawa, H Yokokawa, K Nishimura, N Nakamura, H Mochizuki, T Kato, K Ishii, T Wakita
Organizer
18th International Meeting on Hepatitis C and Related Viruses
Place of Presentation
Seattle Sheraton Hotel, Seattle, USA
Related Report
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[Presentation] Identification of lipid droplet-associated membrane proteins that are involved in HCV production2011
Author(s)
H Aizaki, Y Matsumoto, K Goto, K Watashi, R Suzuki, M Fukasawa, K Hanada, S Sato, N Takahashi, Y Matsuura, K Motojima, T Miyamura, T Suzuki , T Wakita
Organizer
XV International Congress of Virology
Place of Presentation
Sapporo Convention Center, Sapporo, Japan
Related Report
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[Presentation] Antiviral activity of glycyrrhizic acid against hepatitis C virus in vitro2011
Author(s)
Y Matsumoto, K Watashi, R Suzuki, T Matsuura, T Suzuki, T Miyamura, K Wake, T Wakita , H Aizaki
Organizer
XV International Congress of Virology
Place of Presentation
Sapporo Convention Center, Sapporo, Japan
Related Report
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