| Project/Area Number |
23659418
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAMI Hironori 大阪大学, 連合小児発達学研究科, 寄附講座教授 (20325369)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 分子血管病態学 / 高血圧 / アンジオテンシンII / ワクチン / 血圧 |
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| Research Abstract |
Vaccines have been recently developed to treat various diseases such as cancer, rheumatoid arthritis and Alzheimer’s disease. However, before use in the clinical setting, vaccines targeting self-antigens must be demonstrated to be effective and safe, evoking an adequate humoral immune response from B cells. In this study, we evaluated the efficiency and safety of an Ang II vaccine in mice and rats. Immunization with Ang II conjugated to KLH successfully induced the production of anti-Ang II antibody. In an Ang II-infused model, the non-immunized mice showed high blood pressure (BP), whereas the immunized mice (Ang II-KLH) showed a significant decrease in systolic BP. In terms of DNA vaccine, plasmid vector encoding hepatitis B core (HBc)- Ang II fusion protein was injected into spontaneously hypertensive rats (SHR). Anti-Ang II antibody was successfully produced in HBc-Ang II group, and antibody titers were sustained for at least 6 months. Systolic BP was consistently lower in HBc-Ang II group after immunization.
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