Molecular mechanism of alveolar formation
| Project/Area Number |
23659428
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Chiba University |
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Principal Investigator |
HATANO Masahiko 千葉大学, 大学院・医学研究院, 教授 (20208523)
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| Co-Investigator(Kenkyū-buntansha) |
ARIMA Masafumi 千葉大学, 大学院・医学研究院, 講師 (00202763)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 閉塞性肺疾患 / DA-Raf / 肺胞形成 / 筋繊維芽細胞 / II型肺胞細胞 / ノックアウトマウス / Ras/ERKシグナル / 2型肺胞上皮細胞 |
|---|
| Research Abstract |
We analyzed molecular mechanism of alveolization using DA-Raf KO mice. DA-RafKO mice have defects in alveolar septum formation and alveolization during neonatalperiods. Myofibroblast differentiation was severely blocked in P2～P5 of development inthe KO mice. DA-Raf mRNA is highly expressed in type II alveolar epithelial cells(AEII). Lineage trace experiment using SPC-CreER and LacZ floxed mice revealedthat AEII cells did not differentiate to myofibroblasts in neonatal periods. Thus, DARafregulates myofibroblast differentiation by suppressing Ras-ERK cascade in noncellautonomousmanner in neonatal period of lung development.
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Report
(3 results)
Research Products
(35 results)
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[Journal Article] ADAR1 induces adenosine-targeted DNA mutations in senescent Bcl6-deficient cells2013
Author(s)
Tsuruoka, N., Arima, M., Yoshida, N., Okada, S., Sakamoto, A., Hatano, M., Satake, H., Arguni, E., Wang, J.-Y., Yang, J.-H., Nishikura, K., Sekiya, S., Shozu, M. and Tokuhisa, T.
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Journal Title
J. Biol. Chem.
Volume: 288
Issue: 2
Pages: 826-836
DOI
Related Report
Peer Reviewed
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[Journal Article] BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signalling in angiogenesis2012
Author(s)
Ohnuki H, Inoue H, Takemori N, Nakayama H, Sakaue T, Fukuda S, Miwa D, Nishiwaki E, Hatano M, Tokuhisa T, Endo Y, Nose M, and Higashiyama S
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Journal Title
Blood
Volume: 119
Issue: 11
Pages: 2688-2698
DOI
Related Report
Peer Reviewed
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[Journal Article] BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signaling in angiogenesis2012
Author(s)
Ohnuki H, Inoue H, Takemori N, Nakayama H, Sakaue T, Fukuda S, Miwa D, Nishiwaki E, Hatano M, Tokuhisa T, Endo Y, Nose M, Higashiyama S
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Journal Title
Blood
Volume: 119
Pages: 2688-98
Related Report
Peer Reviewed
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[Journal Article] Bcl6 in pulumonary epithelium coordinately controls the expression of the CC-type chemokine genes and attenuates allergic airway inflammation2011
Author(s)
Seto T, Yoshitake M, Ogasawara T, Ikari J, Sakamoto A, Hatano M, Hirata H, Fukuda T, Kuriyama T, Tatsumi K, Tokuhisa T, Arima M
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Journal Title
Clin Exp Allergy
Volume: 41
Pages: 1568-78
Related Report
Peer Reviewed
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