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Molecular mechanism of alveolar formation

Research Project

Project/Area Number 23659428
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Respiratory organ internal medicine
Research InstitutionChiba University

Principal Investigator

HATANO Masahiko  千葉大学, 大学院・医学研究院, 教授 (20208523)

Co-Investigator(Kenkyū-buntansha) ARIMA Masafumi  千葉大学, 大学院・医学研究院, 講師 (00202763)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords閉塞性肺疾患 / DA-Raf / 肺胞形成 / 筋繊維芽細胞 / II型肺胞細胞 / ノックアウトマウス / Ras/ERKシグナル / 2型肺胞上皮細胞
Research Abstract

We analyzed molecular mechanism of alveolization using DA-Raf KO mice. DA-RafKO mice have defects in alveolar septum formation and alveolization during neonatalperiods. Myofibroblast differentiation was severely blocked in P2~P5 of development inthe KO mice. DA-Raf mRNA is highly expressed in type II alveolar epithelial cells(AEII). Lineage trace experiment using SPC-CreER and LacZ floxed mice revealedthat AEII cells did not differentiate to myofibroblasts in neonatal periods. Thus, DARafregulates myofibroblast differentiation by suppressing Ras-ERK cascade in noncellautonomousmanner in neonatal period of lung development.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (35 results)

All 2013 2012 2011 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (25 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Integration of the mouse sperm fertilization-related protein equatorin into the acrosome during spermatogenesis as revealed by super-resolution and immunoelectron microscopy2013

    • Author(s)
      Ito C, Yamatoya K, Yoshida K, Fujimura L, Hatano M, Miyado K, Toshimori K
    • Journal Title

      Cell Tissue Res

      Volume: 352(3) Issue: 3 Pages: 739-750

    • DOI

      10.1007/s00441-013-1605-y

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ADAR1 induces adenosine-targeted DNA mutations in senescent Bcl6-deficient cells2013

    • Author(s)
      Tsuruoka, N., Arima, M., Yoshida, N., Okada, S., Sakamoto, A., Hatano, M., Satake, H., Arguni, E., Wang, J.-Y., Yang, J.-H., Nishikura, K., Sekiya, S., Shozu, M. and Tokuhisa, T.
    • Journal Title

      J. Biol. Chem.

      Volume: 288 Issue: 2 Pages: 826-836

    • DOI

      10.1074/jbc.m112.365718

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of tissue-specific H-2K(d) transgenic mice for the study of K(d)-restricted malaria epitope-specific CD8(+) T-cell responses in vivo2013

    • Author(s)
      Huang J, Li X, Kohno K, Hatano M, Tokuhisa T, Murray PJ, Brocker T, and Tsuji M
    • Journal Title

      J. Immunol. Methods

      Volume: 387 Issue: 1-2 Pages: 254-261

    • DOI

      10.1016/j.jim.2012.10.019

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signalling in angiogenesis2012

    • Author(s)
      Ohnuki H, Inoue H, Takemori N, Nakayama H, Sakaue T, Fukuda S, Miwa D, Nishiwaki E, Hatano M, Tokuhisa T, Endo Y, Nose M, and Higashiyama S
    • Journal Title

      Blood

      Volume: 119 Issue: 11 Pages: 2688-2698

    • DOI

      10.1182/blood-2011-03-345306

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] BAZF, a novel component of cullin3-based E3 ligase complex, mediates VEGFR and Notch cross-signaling in angiogenesis2012

    • Author(s)
      Ohnuki H, Inoue H, Takemori N, Nakayama H, Sakaue T, Fukuda S, Miwa D, Nishiwaki E, Hatano M, Tokuhisa T, Endo Y, Nose M, Higashiyama S
    • Journal Title

      Blood

      Volume: 119 Pages: 2688-98

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of Prickle1 and Prickle2 in neurite outgrowth in murine neuroblastoma cells2012

    • Author(s)
      Fujimura L, Hatano M
    • Journal Title

      Methods Mol Biol

      Volume: 838 Pages: 173-85

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bcl6 in pulmonary epithelium coordinately controls the expression of the CC-type chemokine genes and attenuates allergic airway inflammation2011

    • Author(s)
      Seto T, et al
    • Journal Title

      Clin Exp Allergy

      Volume: 41 Issue: 11 Pages: 1568-78

    • DOI

      10.1111/j.1365-2222.2011.03836.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bcl6 in pulumonary epithelium coordinately controls the expression of the CC-type chemokine genes and attenuates allergic airway inflammation2011

    • Author(s)
      Seto T, Yoshitake M, Ogasawara T, Ikari J, Sakamoto A, Hatano M, Hirata H, Fukuda T, Kuriyama T, Tatsumi K, Tokuhisa T, Arima M
    • Journal Title

      Clin Exp Allergy

      Volume: 41 Pages: 1568-78

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Ras-ERK カスケードのアンタゴニストDA-Raf は肺胞形成を制御している2012

    • Author(s)
      渡邊-高野晴子, 幡野雅彦, 坂本明美, 高野和儀, 徳久剛史, 遠藤剛
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Nczf disruption results in early embryonic lethality by disregurating p27 expression2012

    • Author(s)
      寺竹洋一、長谷川勇太,藤村理紗、高野晴子、徳久剛史,幡野雅彦
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] Nitric oxide derived from enteric neurons regulates intestinal barrier function2012

    • Author(s)
      藤村理紗、坂本明美、有馬雅史、徳久剛史、幡野雅彦
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Bcl6 is required for ICOSL expression on activated B cells2012

    • Author(s)
      飯島裕太、Pan Jing、河野麻仁、有馬雅史、幡野雅彦、徳久剛史、坂本明
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] A role of Bcl6 in differentiation of memory precursor CD8 T cells2012

    • Author(s)
      Pan Jing、坂本明美、河野麻仁、有馬雅史、幡野雅彦、徳久剛史
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of IL-33 in a suppressive function of Bcl6 for IL-4 production by memory Th2 cells2012

    • Author(s)
      有馬雅史、谷口俊文、藤村理紗、坂本明美、幡野雅彦、徳久剛史
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Regulation of SLAM-associated protein in follicular helper T cells by Bcl62012

    • Author(s)
      谷口俊文、坂本明美、幡野雅彦、松本健司、斎藤博久、徳久剛史、有馬雅史
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Roles of ADAR1 in somatic hypermutation and class switch recombination in germinal center B cells.2012

    • Author(s)
      鶴岡信栄、吉田信也、成毛耕儀、有馬雅史、坂本明美、幡野雅彦、王継揚、徳久剛史
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Phf11 promotes IgE class switch recombination in activated B cells2012

    • Author(s)
      山本拓也、谷口俊文、稲嶺絢子、藤村理紗、坂本明美、幡野雅彦、徳久剛史、有馬雅史
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Year and Date
      2012-12-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Nczf disruption results in early embryonic lethality by disregurating p27 expression2011

    • Author(s)
      寺竹洋一、長谷川勇太,藤村理紗、高野晴子、徳久剛史,幡野雅彦
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Ncx 欠損は腸管上皮のバリア異常により腸炎を発症する2011

    • Author(s)
      藤村理紗、坂本明美、有馬雅史、徳久剛史、幡野雅彦
    • Organizer
      第40回日本免疫学
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] エフェクターとセントラルメモリーの分化はナイーブCD4 T細胞の段階で決定されている2011

    • Author(s)
      河野麻仁、坂本明美、Pan Jing、山下公大、稲毛寿美奈、有馬雅史、幡野雅彦、徳久剛史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 長期生存型抗体産生細胞分化におけるIL-21 シグナルの役割2011

    • Author(s)
      稲嶺絢子、有馬雅史、吉田信也、坂本明美、幡野雅彦、岡本美孝、徳久剛史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 有馬雅史好塩基球のTH2サイトカイン遺伝子発現制御におけるGATA2 の役割2011

    • Author(s)
      花角尚子、稲嶺絢子、小橋優子、高野晴子、藤村理紗、坂本明美、幡野雅彦、徳久剛史、有馬雅史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 樹状細胞によるTH2 誘導サイトカイン産生に対するBcl6 の抑制作用2011

    • Author(s)
      小橋優子、稲嶺絢子、稲毛寿美奈、高野晴子、藤村理紗、坂本明美、幡野雅彦、徳久剛史、有馬雅史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Bcl6はDCからのTNFa 産生を抑制することによりTH分化を制御する2011

    • Author(s)
      稲毛寿美奈、坂本明美、河野麻仁、Pan Jing、有馬雅史、幡野雅彦、徳久剛
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] メモリープリカーサーCD8 T 細胞分化におけるBcl6の役割の解析2011

    • Author(s)
      Pan Jing、坂本明美、河野麻仁、稲毛寿美奈、有馬雅史、幡野雅彦、徳久剛史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-28
    • Related Report
      2012 Final Research Report
  • [Presentation] TFH 細胞とTH2 細胞におけるIL-4 は異なる調節機構で産生される2011

    • Author(s)
      有馬雅史、稲嶺絢子、小橋優子、坂本明美、幡野雅彦、久保允人、徳久剛史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] ADAR1 はBcl6 欠損細胞において体細胞突然変異を誘導する2011

    • Author(s)
      鶴岡信栄、有馬雅史、坂本明美、幡野雅彦、徳久剛史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 胚中心B 細胞のA-T 塩基対変異におけるADAR1 の役割2011

    • Author(s)
      吉田信也、鶴岡信栄、成毛耕儀、有馬雅史、高野晴子、坂本明美、幡野雅彦、徳久剛史
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Nczf disruption results in early embryonic lethality by disregulating p27 expression2011

    • Author(s)
      寺竹洋一,長谷川勇太、藤村理紗、高野晴子、徳久剛史,幡野雅彦
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] Ncx欠損は腸管上皮バリアの異常により腸炎を発症する2011

    • Author(s)
      藤村理紗、坂本明美、有馬雅史、徳久剛史、幡野雅彦
    • Organizer
      第40回日本免疫学会
    • Place of Presentation
      千葉
    • Related Report
      2011 Research-status Report
  • [Presentation] Ras-ERKカスケードのアンタゴニストDA-Rafは肺胞形成を制御している

    • Author(s)
      渡邊-高野 晴子, 幡野 雅彦, 坂本 明美, 高野 和儀, 徳久 剛史, 遠藤 剛
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nczf disruption results in early embryonic lethality by disregulating p27 expression.

    • Author(s)
      寺竹洋一、長谷川勇太,藤村理紗、高野晴子、徳久剛史,幡野雅彦
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nitric oxide derived from enteric neurons regulates intestinal barrier function.

    • Author(s)
      藤村理紗、坂本明美、有馬雅史、徳久剛史、幡野雅彦
    • Organizer
      第41回日本免疫学会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Book] "Methods in Molecular Biology"Edited by Chen P. and Turksen K. Springer2012

    • Author(s)
      Fujimura L. and Hatano M.
    • Publisher
      Role of Prickle1 and Prickle2 in neurite outgrowth in murine neuroblastoma cells. Planar cell polarity methods and protocols.
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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