Study for novel biomarkers for Alzheimer's disease
Project/Area Number |
23659450
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurology
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Research Institution | Hirosaki University |
Principal Investigator |
SHOJI Mikio 弘前大学, 医学(系)研究科(研究院), 教授 (60171021)
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Co-Investigator(Renkei-kenkyūsha) |
MATSUBARA Etsuro 弘前大学, 医学(系)研究科(研究院), 准教授 (70219468)
KAWARABAYASHI Takeshi 弘前大学, 医学部附属病院, 講師 (90186156)
WAKASAYA Yasuhito 弘前大学, 医学部附属病院, 助教 (10598725)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 神経分子病態学 / 神経内科学 / 神経科学 / 臨床神経学 / アルツハイマー病 / バイオマーカー |
Research Abstract |
To facilitate early diagnosis of AD dementia and other non-AD dementias, simple and quick assay of CSF and plasma biomarkers for Alzheimer disease and non-AD dementia were studied and developed. 1) To identify presence of plasma tau and p-tau, anti-human CNS tau antibodies (tauA/tauB) were developed. Western blot analysis of immunoprecipitated samples from CSF and plasma of TgtauP301L mice using tauA/tauB revealed the real presence of metabolized tau fragments from CNS and PNS. Further confirmation of the presence of CNS tau was necessary. Basic evaluation of CSF and plasma α-synuclein showed adequate reproducibility and sensitivity. Measurements of 105 cases of AD and non-AD dementias showed that increased levels of MCI, and other neurological diseases CSF As40,As42, tauおよび血液As40,As42測定値in AD, MCI, and controls, but, decreased levels of α-synuclein in Parkinson diseases. The levels of CSF As40, As42, tau andplasma As40,As42were correlated with those levels of α-synuclein. High sensitive ELISA of TDP-43 are developing. Candidate 28 molecules as Alzheimer disease identified by proteomics analysis of CSF proteins with more than 30,000 kD were further evaluated using bioinformatics data base for patent filing.
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] SORL1 Is Genetically Associated with Late-Onset Alzheimer’s Disease in Japanese, Koreans and Caucasians2013
Author(s)
Miyashita A, Koike A, Jun G, Kawarabayashi T, Shoji M,Arai H, Asada T, Harigaya Y, Ikeda M, Amari M, Ikeuchi T, St. George-Hyslop P,
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Journal Title
PLoS One
Volume: 8
Issue: 4
Pages: e58618-e58618
DOI
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Peer Reviewed
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[Journal Article] Altered Y-secretase activity in mild cognitive impairment and Alzheimer's disease2012
Author(s)
Kakuda N, Shoji M, Arai H, Furukawa K, Ikeuchi T, Akazawa K, Takami M, Hatsuta H, Murayama s, Hashimoto Y, Miyajima M, Arai H, Nagashima Y Yamaguchi H, Kuwano R, Nagaike K, Ihara Y ; the Japanese Alzheimer's Disease Neuroimaging Initiative
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Journal Title
Eur J Neurol
Volume: 4
Issue: 4
Pages: 344-352
DOI
Related Report
Peer Reviewed
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[Journal Article] Extracellular and intraneuronal HMW-AbetaOs represent a molecular basis of memory loss in Alzheimer's disease model mouse2011
Author(s)
Takamura A, Okamoto Y, Kawarabayashi T, Yokoseki T, Shibata M, Mouri A, Nabeshima T, Sun H, Abe K, Urisu T, Yamamoto N, Shoji M, Yanagisawa K, Michikawa M, Matsubara E
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Journal Title
Mol Neurodegener
Volume: 6
Issue: 1
Pages: 20-20
DOI
Related Report
Peer Reviewed
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[Journal Article] Dissociation of beta-amyloid from lipoprotein in cerebrospinal fluid from Alzheimer' s disease accelerates beta-amyloid-422011
Author(s)
Takamura A, Kawarabayashi T, Yokoseki T, Shibata M, Morishima-Kawashima M, Saito Y, Murayama S, Ihara Y, Abe K, Shoji M, Michikawa M, Matsubara E
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Journal Title
assembly. Neurosci Res
Volume: 89
Issue: 6
Pages: 815-821
DOI
Related Report
Peer Reviewed
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[Journal Article] Factors responsible for neurofibrillary tangles and neuronal cell losses in tauopathy.2011
Author(s)
Wakasaya Y, Kawarabayashi T, Watanabe M, Yamamoto-Watanabe Y, Takamura A, Kurata T, Murakami T, Abe K, Yamada K, Wakabayashi K, Sasaki A, Westaway D, Hyslop PS, Matsubara E, Shoji M
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Journal Title
J Neurosci Res
Volume: 89(4)
Issue: 4
Pages: 576-84
DOI
Related Report
Peer Reviewed
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[Presentation] アルツハイマー病2012
Author(s)
東海林幹夫
Organizer
第1回認知症研修会in米子
Place of Presentation
ランチョンセミナー
Year and Date
2012-04-07
Related Report
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[Presentation] 認知症と薬物療法の進歩2011
Author(s)
東海林幹夫
Organizer
第20回日本意識障害学会,ランチョンセミナー
Place of Presentation
弘前ベストウエスタンホテル
Year and Date
2011-09-03
Related Report
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