The role of the elongation of long chain fatty acids through the cannabinoid cascade in osteoclasts

• Project/Area Number: 23659473
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: University of Tsukuba
• Principal Investigator: ISHII Kiyoaki 筑波大学, 医学医療系, 助教 (80419150)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エネルギー代謝 / 骨粗鬆症

Research Abstract

In BMM of Elovl6 KO, it is promotion of proliferation and differentiation. In addition, activation of the bone resorption function in mature osteoclasts was observed. At this time, there was no change with a typical differentiation marker, but expression of the gene relevant to a cell cycle was increasing. Furthermore, proliferation and differentiation were accelerating also in osteoblasts. Therefore, the composition change of fatty acid affects energy metabolism and a cell cycle, and is considered to adjust proliferation and differentiation.

Research Products

• [Journal Article] Metabolic regulation of osteoclast differentiation and function (2013)
  • Author(s): Indo Y, Takeshita S, Ishii K, Hoshii T, Aburatani H, Hirao A, Ikeda K
  • Journal Title: J Bone Miner Res Volume: 28 Issue: 11 Pages: 2392-2399
  • DOI: 10.1002/jbmr.1976
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Inhibition of autophagy enhances sunitinib-induced cytotoxicity in rat pheochromocytoma PC12 cells (2013)
  • Author(s): Ikeda T, Ishii KA, Miura M, Saito Y, Otagiri A, Kawakami Y, Shimano H, Hara H, Takekoshi K
  • Journal Title: J Pharmacol Sci. Volume: 121 Pages: 67-73
  • NAID: 10031147588
  • Peer Reviewed
• [Journal Article] TFE3 inhibits myoblast diffrentiation in C2C12 cells via down-regulating gene expression of myogenin. (2013)
  • Author(s): Naka A, et al.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 430 Issue: 2 Pages: 664-669
  • DOI: 10.1016/j.bbrc.2012.11.094
  • Peer Reviewed / Open Access
• [Journal Article] Sunitinib inhibits catecholamine synthesis and secretion in pheochromocytoma tumor cells by blocking vegf receptor 2 via plc-7-related pathways (2012)
  • Author(s): Yuichi Aita, Kiyo-aki Ishii, Yuria Saito, Tatsuhiko Ikeda, Yasushi Kawakami, Hitoshi Shimano, Hisato Hara, Kazuhiro Takekoshi
  • Journal Title: American Journal of Physiology-Endocrinology and Metabolism Volume: 303 Issue: 8 Pages: E1006-E1014
  • DOI: 10.1152/ajpendo.00156.2012
  • Peer Reviewed
• [Journal Article] Elovl6 promotes nonalcoholic steatohepatitis (2012)
  • Author(s): Takashi Matsuzaka, et al
  • Journal Title: Hepatology Volume: 56 Issue: 6 Pages: 2199-2208
  • DOI: 10.1002/hep.25932
  • Peer Reviewed
• [Journal Article] Identical germline mutations in the TMEM127 gene in two unrelated Japanese patients with bilateral pheochromocytoma (2012)
  • Author(s): Takeichi, N.
  • Journal Title: Clini Endocrinol. Volume: 77 Issue: 5 Pages: 707
  • DOI: 10.1111/j.1365-2265.2012.04421.x
  • Peer Reviewed
• [Journal Article] Dicer has a crucial role in the early stage of adipocyte differentiation, but not in lipid synthesis, in 3T3-L1 cells (2012)
  • Author(s): Fujimoto Y, Nakagawa Y, Shingyouchi A, Tokushige N, Nakanishi N, Satoh A, Matsuzaka T, Ishii KA, Iwasaki H, Kobayashi K, Yatoh S, Suzuki H, Yahagi N, Urayama O, Yamada N, Shimano H
  • Journal Title: Biochem Biophys Res Commun Volume: 420 Pages: 931-6
  • Peer Reviewed
• [Journal Article] TFE3 Regulates Muscle Metabolic Gene Expression, Increases Glycogen Stores, and Enhances Insulin Sensitivity in Mice (2012)
  • Author(s): Iwasaki H, Naka A, et al
  • Journal Title: Am J Physiol Endocrinol Metab Volume: 302(7) Issue: 7 Pages: E896-E902
  • DOI: 10.1152/ajpendo.00204.2011
  • Peer Reviewed
• [Journal Article] Sunitinib induces apoptosis in pheochromocytoma tumor cells by inhibiting VEGFR2/Akt/mTOR/S6K1 pathways through modulation of Bcl-2 and BAD (2012)
  • Author(s): Saito Y, et al.
  • Journal Title: American Journal of Physiology Endocrinology and Metabolism Volume: 302 Issue: 6 Pages: 615
  • DOI: 10.1152/ajpendo.00035.2011
  • Peer Reviewed
• [Journal Article] Sunitinib Inhibits Catecholamine Synthesis and Secretion in Pheochromocytoma Tumor Cells by Blocking VEGF Receptor 2 via PLC-γ-related Pathways. (2012)
  • Author(s): Aita Y, Ishii KA et al.
  • Journal Title: American Journal of Physiology Endocrinology and Metabolism Volume: 303 (8)
  • Peer Reviewed
• [Journal Article] Macrophage Elovl6 deficiency ameliorates foam cell formation and reduces atherosclerosis in low-density lipoprotein receptor-deficient mice (2011)
  • Author(s): Saito R, Matsuzaka T, Karasawa T, Sekiya M, Okada N, Igarashi M, Matsumori R, Ishii K, Nakagawa N, Kobayashi K, Yatoh S, Takahashi A, Sone H, Suzuki H, Yahagi N, Yamada N Shimano H
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 31 Issue: 9 Pages: 1973-9
  • DOI: 10.1161/atvbaha.110.221663
  • Peer Reviewed
• [Journal Article] Sterol regulatory element-binding protein-1 determines plasma remnant lipoproteins and accelerates atherosclerosis in low-density lipoprotein receptor-deficient mice (2011)
  • Author(s): Karasawa T, Takahashi A, Saito R, Sekiya M, Igarashi M, Iwasaki H, Miyahara S, Koyasu S, Nakagawa Y, IshiiK, Matsuzaka T, Kobayashi K, Yahagi N, Takekoshi K, Sone H, Yatoh S, Suzuki H, Yamada N, Shimano H
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 31 Issue: 8 Pages: 1788-95
  • DOI: 10.1161/atvbaha.110.219659
  • Peer Reviewed
• [Presentation] 小腸における新規転写因子CREB3l3 の制御と生理的役割 (2012)
  • Author(s): 石井清朗
  • Organizer: 第12回TC カンファレンス
  • Place of Presentation: 興和製薬株式会社:東京
  • Year and Date: 2012-07-07
• [Book] 糖尿病イラストレイテッド (2012)
  • Author(s): 石井清朗
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.u-tsukuba-endocrinology.jp/