Development and analysis of model rats for diseases of endocrinology and metabolism
| Project/Area Number |
23659476
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKAO Kazuwa 京都大学, 医学研究科, 教授 (00172263)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 疾患モデルラット / エネルギー代謝 / 脂肪細胞 / レプチン |
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| Research Abstract |
Using the method with ENU (N-ethyl-N-nitrosourea) mutagenesis, we generated a leptin deficient Lep^(mkyo)/Lep^(mkyo)rat that had a nonsense mutation (Q92X) in leptin gene. Lep^(mkyo)/Lep^(mkyo)rats showed obese phenotypes including fatty liver, which werecomparable to Lepob/Lepobmice. Lep^(mkyo)/Lep^(mkyo) rat is the treatable rat model lacking leptin signal and will bring further understanding of obesity and its complications.Mutations in the Berardinelli-Seip congenital lipodystrophy 2 gene (BSCL2) are the underlying defect in patients with congenital generalized lipodystrophy type 2. Using the method with ENU (N-ethyl-N-nitrosourea) mutagenesis, we generated a seipin deficient (seipin KO) rat that had a premature stop codon (L20X) in BSCL2. The present study clearly revealed the physiological roles of seipin not only in adipose tissue but also brain and testis.
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Intracerebroventricular administration of C-type natriuretic peptide suppresses food intake via activation of the melanocortin system in mice.2013
Author(s)
N. Yamada-Goto, G. Katsuura, K. Ebihara, M. Inuzuka, Y. Ochi, Y. Yamashita, T. Kusakabe, A. Yasoda, N. Asahara-Satoh, H. Ariyasu, K. Hosoda, K. Nakao.
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Journal Title
Diabetes
Volume: 62(5)
Issue: 5
Pages: 1500-1504
DOI
NAID
Related Report
Peer Reviewed
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