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Elucidation of energy metabolic pathways in hormone-dependent cancers by utilizing cellular and in vivo imaging analyses

Research Project

Project/Area Number 23659479
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Endocrinology
Research InstitutionSaitama Medical University

Principal Investigator

HORIE Kuniko  埼玉医科大学, 医学部, 准教授 (90261982)

Co-Investigator(Kenkyū-buntansha) IKEDA Kazuhiro  埼玉医科大学, 医学部, 講師 (30343461)
Co-Investigator(Renkei-kenkyūsha) INOUE Satoshi  東京大学, 医学部, 特任教授 (40251251)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords性ホルモン / がん / イメージング / エネルギー代謝 / ホルモン依存性がん / 酸化的リン酸化 / エストロゲン / アンドロゲン / 代謝 / 蛍光蛋白質
Research Abstract

In order to reveal characterize metabolic pathways involved in the proliferation and progression of hormone-dependent cancers such as breast and prostate cancers, cell models of hormone-naive and hormone-resistant cancer cellsexpressing fluorescent reporters were generated. A microscopic imaging system for the observation of these cancer cells was developed in response to environmental alterationsby monitoring reporter fluorescence. Inoculation of cancer cells stably expressing luciferase reporter genes into nude mice was performed and the dynamics of cancer cell proliferation and metastasis were investigated based on in vivo imaging analysis. Expression and transcriptional regulation of metabolic genes in hormone-dependent cancer cells were analyzed molecular biologically. Taken together, the present study identified novel molecular targets that can be applied to the diagnosis, therapy, and prevention of hormone-dependent cancers.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] enome-wide integrated analyses of androgen receptor signaling in prostate cancer based on the high-throughput technology2013

    • Author(s)
      Horie-Inoue K, Inoue S
    • Journal Title

      Current Drug Targets

      Volume: 14 Issue: 4 Pages: 472-480

    • DOI

      10.2174/1389450111314040008

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Integration of Gene Expression and ChIP-chip Analyses Uncovers Genome-Wide Androgen Receptor Signaling in Prostate Cancer.2013

    • Author(s)
      Horie-Inoue K, Inoue S
    • Journal Title

      Curr Drug Targets

      Volume: 14 Pages: 472-480

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of double-positive FOXA1 and FOXP1 immunoreactivities with favorable prognosis of tamoxifen-treated breast cancer patients2012

    • Author(s)
      Ijichi N, Shigekawa T, Ikeda K,Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S
    • Journal Title

      Horm Cancer

      Volume: 3 Issue: 4 Pages: 147-159

    • DOI

      10.1007/s12672-012-0111-0

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] TACC2 is an androgen-responsive cell cycle regulator promoting androgen-mediated and castration-resistant growth of prostate cancer2012

    • Author(s)
      Takayama K, Horie-Inoue K, Suzuki T, Urano T, Ikeda K, Fujimura T, Takahashi S, Homma Y, Ouchi Y, Inoue S
    • Journal Title

      Mol Endocrinol

      Volume: (in press) Issue: 5 Pages: 748-761

    • DOI

      10.1210/me.2011-1242

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Forkhead box transcription factor, forkhead box A1, shows negative association with lymph node status in endometrial cancer, and represses cell proliferation and migration of endometrial cancer cells2012

    • Author(s)
      Abe Y, Ijichi N, Ikeda K, Kayano H, Horie-Inoue K, Takeda S, Inoue S
    • Journal Title

      Cancer Sci

      Volume: 103 Issue: 4 Pages: 806-812

    • DOI

      10.1111/j.1349-7006.2012.02201.x

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] FOXP1, an estrogen-inducible transcription factor, modulates cell proliferation in breast cancer cells and 5-year recurrence-free survival of patients with tamoxifen-treated breast cancer2011

    • Author(s)
      Shigekawa T, Ijichi N, Ikeda K, Horie-Inoue K, Shimizu C, Saji S, Aogi K, Tsuda H, Osaki A, Saeki T, Inoue S
    • Journal Title

      Horm Cancer

      Volume: 2 Issue: 5 Pages: 286-297

    • DOI

      10.1007/s12672-011-0082-6

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Systemic screening of microRNAs and their targets for breast cancer cells using a comprehensive lentiviral microRNA library and microarrays.2013

    • Author(s)
      Kuniko Horie-Inoue
    • Organizer
      Keystone Symposia: Non-Coding RNAs
    • Place of Presentation
      Snowbird Resort (Utah, USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Systemic screening of microRNAs nad their targets for breast cancer cells using a comprehensive lentiviral microRNA library and microarrays.2012

    • Author(s)
      Horie-Inoue K, Yamaga R, Okamoto K, Ikeda K, Inoue S.
    • Organizer
      Keystone Symposia, Non-coding RNAs
    • Place of Presentation
      Snowbird, Utah, USA.
    • Related Report
      2012 Final Research Report
  • [Presentation] Cytochrome c oxidase subunit 7a related polypeptide COX7RP is an estrogen target gene that plays a role in the OXPHOS pathway.2011

    • Author(s)
      Ikeda K, Horie-Inoue K, Inoue S
    • Organizer
      5th Biennial Meeting of Society for Free Radical Research-Asia (SFRR-Asia), 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM), 11th Conference of Japanese Society of Mitochondrial Research and Medicine (J-mit)
    • Place of Presentation
      鹿児島市
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of androgen-regulated cytochrome coxidase-related genes in hormone-sensitive prostate cancer cells through RNA-sequencing and systemic chromatin immunoprecipitation analysis2011

    • Author(s)
      Horie-Inoue K, Takayama K, Ikeda K, Suzuki Y, Inoue S
    • Organizer
      Mitochondrial Assembly & Dynamics in Health, Disease & Aging, FASEB Summer Research Conferences
    • Place of Presentation
      Steamboat Springs, Colorado, USA.
    • Related Report
      2012 Final Research Report
  • [Presentation] dentification of androgen-regulated cytochrome c oxidase-related genes in hormone-sensitive prostate cancer cells through RNA-sequencing and systemic chromatin immunoprecipitation analysis.2011

    • Author(s)
      Kuniko Horie-Inoue
    • Organizer
      FASEB Summer Research Conferences: Mitochondrial Assembly and Dynamics in Health, Disease and Aging
    • Place of Presentation
      Steamboat Springs (Colorado, USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] Cytochrome c oxidase subunit 7a related polypeptide COX7RP is an estrogen target gene that plays a role in the OXPHOS pathway.2011

    • Author(s)
      Kazuhiro Ikeda
    • Organizer
      8th Conference of Asian Society for Mitochondrial Research and Medicine
    • Place of Presentation
      Kagoshima Citizens' Culture Hall (Kagoshima, Japan)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.saitama-med.ac.jp/genome/Div02_GRST/index.html

    • Related Report
      2012 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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