| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Direct reprogramming of somatic cells into hematopoietic stem cells is challenging and has not been yet achieved. In this study, I tried to reprogram an E2A-deficient pro-B cell line, which retain multipotent differentiation capacity, into hematopoietic stem cells. Various combinations of key transcription factors known to be essential for hematopoietic stem cells were transduced into E2A-deficient pro-B cells and cultured on stromal cells in a medium which supports hematopoietic stem cells. I observed generation of myeloid cells expanding in culture, but failed to obtain hematopoietic stem cells in this trial. To achieve the real reprogramming, more technical renovation is definitely needed.
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