Analysis of relationship between nuclear segmentation and gene expression using neutrophils and ATL cells

• Project/Area Number: 23659485
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: KITAMURA Toshio 東京大学, 医科学研究所, 教授 (20282527)
• Co-Investigator(Kenkyū-buntansha): UCHIMARU Kaoru 東京大学, 医科学研究所, 准教授 (60251203)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 好中球 / ATL / エピジェネティクス / メチル化 / 遺伝子発現 / DNAメチル化

Research Abstract

Multi-step oncogenesis of HTLV-1 infected cells to Adult T-cell leukemia well reflected on the cell surface expression of CD7 and CADM1, tumor suppressor gene originally detected in lung cancer. It is suggested that alteration of gene expression in this process is regulated by methylation of the gene. We can detect intermediate cells of oncogenesis even in the peripheral blood of asymptomatic carriers.

Research Products

• [Journal Article] The CD3 versus CD7 plot in multicolor flow cytometry reflects progression of disease stage in patients infected with HTLV-I. (2013)
  • Author(s): Kobayashi S, Tian Y, Ohno N, Yuji K, Ishigaki T, Isobe M, Ohfuchi-Tsuda M, Oyaizu N, Watanabe E, Watanabe N, Tani K, Tojo A and Uchimaru K.
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e53728-e53728
  • DOI: 10.1371/journal.pone.0053728
  • Peer Reviewed
• [Journal Article] Development of peripheral T-cell lymphoma not otherwise specified in an HTLV-1 carrier. (2013)
  • Author(s): Ishigaki T, Isobe M, Kobayashi S, Yuji K, Ohno N, Watanabe N, Tojo A and Uchimaru K.
  • Journal Title: Int J Heamtol. Volume: prepublished online Issue: 5 Pages: 667-672
  • DOI: 10.1007/s12185-013-1314-z
  • NAID: 10031176497
  • Peer Reviewed
• [Journal Article] Adult T-cell leukemia cells are characterized by abnormalities of Helios expression that promote T-cell growth. (2013)
  • Author(s): Asanuma S
  • Journal Title: Cancer Sci. Volume: in press.
  • Peer Reviewed
• [Journal Article] Polycomb- Mediated Loss of miR-31 Activates NIK- dependent NF-kappa B Pathway in Adult T-cell Leukemia and Other Cancers (2012)
  • Author(s): Makoto Yamagishi, Kazumi Nakano, Ariko Miyake, Tadanori Yamochi, Yayoi Kagami, Akihisa, Tsutsumi, Yuka Matsuda, Aiko Sato-Otsubo, Satsuki Muto, Atae Utsunomiya, Kazunari Yamaguchi, Kaoru Uchimaru, Seishi Ogawa, and Toshiki Watanabe
  • Journal Title: Cancer cell Volume: 21(1) Pages: 121-135
• [Journal Article] Leukemic T cells are specifically enriched in a unique CD3^dimCD7^low subpopulation of CD4^+ T cells in acute-type adult T cell leukemia (2011)
  • Author(s): Tian Y, Kobayashi S, Ohno N, Isobe M, Tsuda M, Tani K, Zaike Y, Watanabe N, Tojo A and Uchimaru K
  • Journal Title: Cancer Sci Volume: 102(3) Pages: 569-577
• [Journal Article] Adult T-cell leukemia cells are characterized by abnormalities of Helios expression that promote T-cell growth
  • Author(s): Asanuma S, Yamagishi M, Kawanami Ka, Nakano K, Sato-Otsubo A, Muto S, Sanada M, Yamochi T, Kobayashi S, Utsunomiya A, Iwanaga M, Yamaguchi K, Uchimaru K, Ogawa S and Watanabe T
  • Journal Title: Cancer Sci Volume: (in press)
• [Presentation] 患者検体を用いたCD7とTSLC1/CADM1のFACS解析はATLの多段階発癌を反映する (2012)
  • Author(s): 小林誠一郎、中野和民、渡辺恵理、石垣知寛、大野伸広、渡辺信和、東條有伸、内丸薫
  • Organizer: 第1回ATLシンポジウム
  • Place of Presentation: 東京
• [Presentation] TSLC1/CD7を用いた造血細胞移植後のATL細胞のモニタリング (2012)
  • Author(s): 石垣知寛、小林誠一郎、大野伸広、渡辺恵理、田野崎隆二、渡辺信和、東條有伸、内丸薫
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] 同種造血幹細胞移植を見据えたATLの治療戦略:その後方視的解析 (2012)
  • Author(s): 大野伸広、田野崎隆二、小林誠一郎、石垣知寛、渡辺信和、内丸薫
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] Molecular Hallmarks of Adult T cell Leukemia: miRNA, Epigenetics, and Emerging Signaling Abnormalities (2012)
  • Author(s): Makoto Yamagishi, Ryutaro Takahashi, Kazumi Nakano, Satomi Asanuma, Atae Utsunomiya, Kazunari Yamaguchi, Kaoru Uchimaru, Seishi Ogawa, and Toshiki Watanabe
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] CD7 vs CADM1 in FACS reflects multi-step oncogenesis of ATL and discriminates HTLV-1 infected cells (2012)
  • Author(s): Seiichro Kobayashi, Eri Watanabe, Tomohiro Ishigaki, Nobuhiro Ohno, Koichiro Yuji, Yukio Tsukada, Akihiro Ohmoto, Naoki Shimada, Nobukazu Watanabe, Arinobu Tojo and Kaoru Uchimaru
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] CD3とCD7の展開による急性型ATL細胞の同定:治療後のCD3dimCD7(-)分画のクローナリティ解析 (2012)
  • Author(s): 大野伸広、小林誠一郎、渡辺信和、石垣知寛、湯地晃一郎、東條有伸、内丸薫
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] CD3 vs CD7 plot in multi-colour FACS reflects progression of disease stage of HTLV-1 infected patients (2011)
  • Author(s): Kobayashi S, Tian Y, Ohno N, Isobe M, Tsuda M, Zaike Y, Watanabe N, Tani K, Tojo A Uchimaru K
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋
• [Presentation] フローサイトメトリーによるフェノタイプ解析を用いた、急性型ATLの末梢血腫瘍細胞数の評価 (2011)
  • Author(s): 石垣知寛、在家裕司、小林誠一郎、大野伸広、内丸薫、渡辺信和、小柳津直樹、東條有伸、中内啓光
  • Organizer: 第4回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] マルチカラーFACSにおけるCD3とCD7の展開はHTLV-1感染患者の病期の進行を反映する (2011)
  • Author(s): 小林誠一郎、田亜敏、大野伸広、湯地晃一郎、石垣知寛、磯部優理、津田真由子、在家裕司、渡辺恵理、渡辺信和、谷憲三朗、東條有伸、内丸薫
  • Organizer: 第4回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] CD3 vs CD7 plot in multi-colour FACS reflects progression of disease stage of HTLV-1 infected patients. (2011)
  • Author(s): Kobayashi S
  • Organizer: 第73回日本血液学会総会
  • Place of Presentation: 名古屋国際会議場・名古屋
• [Presentation] マルチカラーFACSにおけるCD3とCD7の展開はHTLV-1感染患者の病期の進行を反映する (2011)
  • Author(s): 小林誠一郎
  • Organizer: 第４回HTLV-1研究会
  • Place of Presentation: 東京大学弥生講堂・東京
• [Presentation] 患者血液検体を用いたCD7とTSLC1/CADM1のFACS解析はATLの多段階発癌を反映する
  • Author(s): 小林誠一郎
  • Organizer: 第1回ATL シンポジウム
  • Place of Presentation: 東京
• [Presentation] TSLC1/CD7を用いた造血細胞移植後のATL細胞のモニタリング
  • Author(s): 石垣知寛
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] 成人T細胞白血病におけるtumor initiating cell の探索の試み
  • Author(s): 矢持忠徳
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] 同種造血幹細胞移植を見据えたATLの治療戦略：その後方視的解析
  • Author(s): 大野伸広
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] 成人T細胞白血病(ATL)における新規TIAM2変異体の同定と遺伝子発現の解析
  • Author(s): 笹島悟史
  • Organizer: 第5回HTLV-1研究会
  • Place of Presentation: 東京
• [Presentation] Molecular Hallmarks of Adult T cell Leukemia: miRNA, Epigenetics, and Emerging Signaling Abnormalities
  • Author(s): Makoto Yamagishi
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] CD7 vs CADM1 in FACS reflects multi-step oncogenesis of ATL and discriminates HTLV-1 infected cells.
  • Author(s): Seiichro Kobayashi
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] Monitoring ATL cells after allogeneic hematopoietic stem cell transplantation with CADM1 and CD7.
  • Author(s): 石垣 知寛
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] CD3とCD7の展開による急性型ATL細胞の同定：治療後のCD3dimCD7(-)分画のクローナリティ解析
  • Author(s): 大野伸広
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
• [Remarks]
  • URL: http://stemcell-u-tokyo.org/sc-si/
• [Patent(Industrial Property Rights)] 患者検体を用いたHTLV-1キャリア、成人T細胞白血病の発癌過程進行度又は悪性度の評価法 (2013)
  • Inventor(s): 内丸 薫、小林誠一郎、渡辺信和
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-034326
  • Filing Date: 2013-02-25
• [Patent(Industrial Property Rights)] 患者検体を用いた HTLV-1 キャリア、成人 T 細胞白血病の発癌過程進行度又 は悪性度の評価法
  • Inventor(s): 内丸薫、小林誠一郎、渡辺信和
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 2013-034326