• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of auto-antigens in acquired aplastic anemia associated with clonal hematopoiesis by hematopoietic stem cells with uniparental disomy of chromosome 6p

Research Project

Project/Area Number 23659486
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionKanazawa University

Principal Investigator

NAKAO Shinji  金沢大学, 医学系, 教授 (70217660)

Co-Investigator(Kenkyū-buntansha) AKATSUKA Yoshiki  藤田保健衛生大学, 医学部, 准教授 (70333391)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords再生不良性貧血 / 6pLOH / 自己抗原 / CLT / HLA-B*40:02 / CTL / 肝炎関連再生不良性貧血 / HLA / 片親性二倍体 / 抗HLA抗体
Research Abstract

To determine the exact prevalence of HLA-A allele-lacking leukocytes (HLA-LLs) in patients with acquired aplastic anemia (AA), we improved the sensitivity of flow cytometry and examined peripheral blood leukocytes of patients with newly-diagnosed AA patients. HLA-LLs were detectable in 6 (28.6%) of 21 patients who were heterozygous with the HLA-A allele. The percentage of HLA-A allele-lacking granulocytes in the total granulocytes ranged from 3.9% to 61.1% (median 8.4%), which was lower than that in patients in remission after immunosuppressive therapy. Cytotoxic T cell (CTL) clones (A6) were successfully established from one of the patients possessing HLA-LLs by stimulating patient’s CD8+T cells with K562 cells transfected with HLA-B*40:02 gene. A6 CTL killed K562 in B*40:02 restricted manner but did not kill other cells including EB virus-transformed lymphoblastoid cell line and Jurkat T cell line transduced with B*40:02.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (4 results)

  • [Journal Article] Increased plasma thrombopoietin levels in patients with myelodysplastic syndrome: a reliable marker for a benign subset of bone marrow failure2013

    • Author(s)
      Seiki Y, Sasaki Y, Hosokawa K, Saito C, Sugimori N, Yamazaki H, Takami A, Nakao S
    • Journal Title

      Haematologica.

      Volume: 98 Issue: 6 Pages: 901-907

    • DOI

      10.3324/haematol.2012.066217

    • NAID

      120005312328

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Individual hematopoietic stem cells in human bone marrow of patients with aplastic anemia or myelodysplastic syndrome stably give rise to limited cell lineages.2013

    • Author(s)
      Katagiri T, et al.
    • Journal Title

      Stem Cells

      Volume: 31 Issue: 3 Pages: 536-46

    • DOI

      10.1002/stem.1301

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Favorable outcome of patients who have 13q deletion: a suggestion for revision of the WHO 'MDS-U' designation.2012

    • Author(s)
      Hosokawa K, et al.
    • Journal Title

      Haematologica

      Volume: 97 Issue: 12 Pages: 1845-9

    • DOI

      10.3324/haematol.2011.061127

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An Epstein-Barr virus-associated leukemic lymphoma in a patient treated with rabbit antithymocyte globulin and cyclosporine for hepatitis-associated aplastic anemia2012

    • Author(s)
      Ohata K
    • Journal Title

      Acta Haematologica

      Volume: Vol.127 Issue: 2 Pages: 96-99

    • DOI

      10.1159/000333609

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frequent loss of HLA alleles associated with copy number-neutral 6pLOH in acquired aplastic anemia2011

    • Author(s)
      Katagiri T., Sato-Otsubo A., Kashiwase K.,Morishima S., Sato Y., Mori Y., Kato M., Sanada M., Morishima Y., Hosokawa K., Sasaki Y., Ohtake S., Ogawa S., Nakao S
    • Journal Title

      Blood

      Volume: 118 Issue: 25 Pages: 6601-6609

    • DOI

      10.1182/blood-2011-07-365189

    • NAID

      120003752541

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aplastic anemia successfully treated with rituximab: the possible role of aplastic anemia-associated autoantibodies as a marker for response2011

    • Author(s)
      Takamatsu H., Yagasaki H., Takahashi Y., Hama A., Saikawa Y., Yachie A., Koizumi S., Kojima S., Nakao S
    • Journal Title

      Eur J Haematol

      Volume: 86 Issue: 6 Pages: 541-545

    • DOI

      10.1111/j.1600-0609.2011.01612.x

    • NAID

      120003141908

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] An Epstein-Barr virus-associated leukemic lymphoma in a patient treated with rabbit antithymocyte globulin and cyclosporine for hepatitis-associated aplastic anemia.2011

    • Author(s)
      Ohata K, Iwaki N, Kotani T, Kondo Y, Yamazaki H, Nakao S
    • Journal Title

      Acta Haematol.

      Volume: 127 Pages: 96-99

    • NAID

      120003752533

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Frequent loss of HLA alleles associated with copy number-neutral 6pLOH in acquired aplastic anemia.2011

    • Author(s)
      Katagiri T, Sato-Otsubo A, Kashiwase K, Morishima S, Sato Y, Mori Y, Kato M, Sanada M, Morishima Y, Hosokawa K, Sasaki Y, Ohtake S, Ogawa S, Nakao S
    • Journal Title

      Blood.

      Volume: 118 Pages: 6601-6609

    • NAID

      120003752541

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aplastic anemia successfully treated with rituximab: the possible role of aplastic anemia-associated autoantibodies as a marker for response.2011

    • Author(s)
      3).Takamatsu H, Yagasaki H, Takahashi Y, Hama A, Saikawa Y, Yachie A, Koizumi S, Kojima S, Nakao S
    • Journal Title

      Eur J Haematol.

      Volume: 86 Pages: 541-545

    • NAID

      120003141908

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] SLIT1 mutation in patients with acquired aplastic anemia: Its relevance in immune pathophysiology2012

    • Author(s)
      Chizuru Saito
    • Organizer
      The American Society of Hematology 54th Annual Meeting
    • Place of Presentation
      GEORGIA WORLD CONGRESS CENTER Atlanta,Georgia, USA
    • Year and Date
      2012-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Frequent loss of HLA alleles from hematopoietic stem cells in patients with hepatitis-associated aplastic anemia.2011

    • Author(s)
      Takamasa Katagiri
    • Organizer
      The American Society of Hematology 53rd Annual Meeting
    • Place of Presentation
      San Diego Convention Center, California, USA.
    • Year and Date
      2011-12-12
    • Related Report
      2012 Final Research Report
  • [Presentation] SLIT1 mutation in patients with acquired aplastic anemia: Its relevance in immune pathophysiology.

    • Author(s)
      Chizuru Saito
    • Organizer
      The American Society of Hematology 54th Annual Meeting
    • Place of Presentation
      GEORGIA WORLD CONGRESS CENTER Atlanta,Georgia,USA.
    • Related Report
      2012 Annual Research Report
  • [Presentation] Frequent loss of HLA alleles from hematopoietic stem cells in patients with hepatitis-associated aplastic anemia.

    • Author(s)
      Takamasa Katagiri, Aiko Sato-Otsubo, Koichi Kashiwase, Satoko Morishima, Yusuke Sato, Yuka Mori, Motohiro Kato, Masashi Sanada, Yasuo Morishima, Shigeki Ohtake, Seishi Ogawa, Shinji Nakao
    • Organizer
      The American Society of Hematology 53rd Annual Meeting,
    • Place of Presentation
      San Diego Convention Center, California, USA.
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi