Identification of auto-antigens in acquired aplastic anemia associated with clonal hematopoiesis by hematopoietic stem cells with uniparental disomy of chromosome 6p
| Project/Area Number |
23659486
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKAO Shinji 金沢大学, 医学系, 教授 (70217660)
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| Co-Investigator(Kenkyū-buntansha) |
AKATSUKA Yoshiki 藤田保健衛生大学, 医学部, 准教授 (70333391)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 再生不良性貧血 / 6pLOH / 自己抗原 / CLT / HLA-B*40:02 / CTL / 肝炎関連再生不良性貧血 / HLA / 片親性二倍体 / 抗HLA抗体 |
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| Research Abstract |
To determine the exact prevalence of HLA-A allele-lacking leukocytes (HLA-LLs) in patients with acquired aplastic anemia (AA), we improved the sensitivity of flow cytometry and examined peripheral blood leukocytes of patients with newly-diagnosed AA patients. HLA-LLs were detectable in 6 (28.6%) of 21 patients who were heterozygous with the HLA-A allele. The percentage of HLA-A allele-lacking granulocytes in the total granulocytes ranged from 3.9% to 61.1% (median 8.4%), which was lower than that in patients in remission after immunosuppressive therapy. Cytotoxic T cell (CTL) clones (A6) were successfully established from one of the patients possessing HLA-LLs by stimulating patient’s CD8+T cells with K562 cells transfected with HLA-B*40:02 gene. A6 CTL killed K562 in B*40:02 restricted manner but did not kill other cells including EB virus-transformed lymphoblastoid cell line and Jurkat T cell line transduced with B*40:02.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Frequent loss of HLA alleles associated with copy number-neutral 6pLOH in acquired aplastic anemia2011
Author(s)
Katagiri T., Sato-Otsubo A., Kashiwase K.,Morishima S., Sato Y., Mori Y., Kato M., Sanada M., Morishima Y., Hosokawa K., Sasaki Y., Ohtake S., Ogawa S., Nakao S
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Journal Title
Blood
Volume: 118
Issue: 25
Pages: 6601-6609
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Frequent loss of HLA alleles associated with copy number-neutral 6pLOH in acquired aplastic anemia.2011
Author(s)
Katagiri T, Sato-Otsubo A, Kashiwase K, Morishima S, Sato Y, Mori Y, Kato M, Sanada M, Morishima Y, Hosokawa K, Sasaki Y, Ohtake S, Ogawa S, Nakao S
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Journal Title
Blood.
Volume: 118
Pages: 6601-6609
NAID
Related Report
Peer Reviewed
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[Presentation] Frequent loss of HLA alleles from hematopoietic stem cells in patients with hepatitis-associated aplastic anemia.
Author(s)
Takamasa Katagiri, Aiko Sato-Otsubo, Koichi Kashiwase, Satoko Morishima, Yusuke Sato, Yuka Mori, Motohiro Kato, Masashi Sanada, Yasuo Morishima, Shigeki Ohtake, Seishi Ogawa, Shinji Nakao
Organizer
The American Society of Hematology 53rd Annual Meeting,
Place of Presentation
San Diego Convention Center, California, USA.
Related Report
