Establishment and dynamism of des-HMGB1, degraded HMGB1 by thrombin-thrombomodulin
| Project/Area Number |
23659491
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Kagoshima University |
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Principal Investigator |
MARUYAMA Ikuro 鹿児島大学, 医歯学総合研究科, 特任教授 (20082282)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAHARA Koichi 大阪工業大学, 工学部, 教授 (10381170)
HASHIGUCHI Teruto 鹿児島大学, 大学院・医歯総合研究科, 教授 (70250917)
OYAMA Yoko 鹿児島大学, 医学部・歯学部附属病院, 特任助教 (20583470)
ITO Takashi 鹿児島大学, 大学院・医歯総合研究科, 特任講師 (20381171)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 血栓 / 止血学 / トロンボモジュリン / トロンビン / HMGB1 / DIC / des-HMGB1 |
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| Research Abstract |
We previously showed that HMGB1 binds to thrombomodulin(TM) and degraded by thrombin-TM at the N-terminus of the molecule cleaving out 10 aminoacdid-residue. We named this degraded HMGB1 as des-HMGB1. We also have showed that des-HMGB1 compete with intact binding to its receptor RAGE, Toll-like receptor-2 and-4 resulting negatively regulating of intact HMGB1 and its receptor signaling.
In this study, we established the des-HMGB1 specific assay ELISA, and investigated its dynamism in various diseases including DIC, sepsis and shock. We showed that the des-HMGB1 was increased in the serum from the patients with these pathologic conditions, especially in the cases treated with recombinant TM. We are now further studying relationship between des-HMGB1 levels and the efficacy of TM treatment and their prognosis.
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Report
(2 results)
Research Products
(16 results)
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[Journal Article] Role of high mobility group box chromosomal protein 1 in ischemia-reperfusion injury in the rat small intestine2012
Author(s)
Kojima M, Tanabe M, Shinoda M, Yamada S, Miyasho T, Suda K, Hibi T, Obara H, Itano O, Kawachi S, Kitajima M, Maruyama I, Kitagawa Y.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Circulating high-mobility group box 1 and cardiovascular mortality in unstable angina and non-ST-segment elevation myocardial infarction2012
Author(s)
Hashimoto T, Ishii J, Kitagawa F, Yamada S, Hattori K, Okumura M, Naruse H, Motoyama S, Matsui S, Tanaka I, Izawa H, Maruyama I, Nomura M, Ozaki Y.
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Journal Title
Atherosclerosis
Volume: 221(2)
Pages: 490-5
Related Report
Peer Reviewed
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[Journal Article] Valproic acid increases susceptibility to endotoxin shock through enhanced release o f high-mobility group box 12011
Author(s)
Sugiura S, Ishihara Y, Komatsu T, Hagiwara M, Tanigawa N, Kato Y, Mizutani H, Kawahara K, Maruyama I, Noguchi T, Matsushita K.
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Journal Title
Shock 2011
Volume: 36(5)
Pages: 494-500
Related Report
Peer Reviewed
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[Journal Article] Gradual increase of high mobility group protein b1 in the lungs after the onset of acute exacerbation of idiopathic pulmonary fibrosis2011
Author(s)
Ebina M, Taniguchi H, Miyasho T, Yamada S, Shibata N, Ohta H, Hisata S, Ohkouchi S, Tamada T, Nishimura H, Ishizaka A, Maruyama I, Okada Y, Takashi K, Nukiwa T.
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Journal Title
Pulm Med
Volume: 2011
Pages: 916486-916486
NAID
Related Report
Peer Reviewed
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