| Project/Area Number |
23659494
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MIYATA Toshiyuki 独立行政法人国立循環器病研究センター, 研究所, 部長 (90183970)
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| Co-Investigator(Kenkyū-buntansha) |
BANNO Fumiaki 独立行政法人国立循環器病研究センター, 研究所, 研究員 (00373514)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 血栓性血小板減少性紫斑病 / ADAMTS13 / 抗血栓因子 / 肝星細胞 / トランスジェニックマウス / Bac-TRAP / 抗血栓性因子 / トランスクリプトーム解析 / バイオイメージング |
|---|
| Research Abstract |
We developed novel BAC transgenic mice with cDNA encoding a fusion protein of a fluorescent protein EGFP and a ribosomal protein L10a under control of the hepatic stellate cell-specific ADAMTS13 promoter. We detected EGFP-L10a fusion protein in the liver of the transgenic mice using Western blot and further observed fusion protein-expressing cells in sinusoid cells.
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