Integration of cell sorting with metagenomic diagnosis system
| Project/Area Number |
23659505
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Osaka University |
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Principal Investigator |
YASUNAGA Teruo 大阪大学, 微生物病研究所, 教授 (20260630)
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| Co-Investigator(Kenkyū-buntansha) |
OISHI Kazunori 大阪大学, 微生物病研究所, 特任教授 (80160414)
NAKAMURA Shota 大阪大学, 微生物病研究所, 助教 (90432434)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | メタゲノム / 感染症診断 / セルソーティング / バイオインフォマティクス |
|---|
| Research Abstract |
We have developed a metagenomic diagnosis system to diagnose infectious diseases by applying metagenomic analysis. This system has enabled us to directly detect pathogenic bacteria, influenza viruses and noroviruses from clinical samples. From those analyses, it has been found that contaminants such as host genome, bacteria, and/ or fungi in clinical samples reduce the efficiency and sensitivity of metagenomic detection. In order to improve the detection efficiency of the system, we developed a basic protocol of the metagenomic diagnosis system using cell-sorting technology.
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Report
(2 results)
Research Products
(24 results)
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[Journal Article] Frequency of D222G and Q223R hemagglutinin mutants of pandemic (H1N1) 2009 influenza virus in Japan between 2009 and 20102012
Author(s)
Yasugi M, Nakamura S, Daidoji T, Kawashita N, Ramadhany R, Yang CS, Yasunaga T, Iida T, Horii T, Ikuta K, Takahashi K, Nakaya T
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Journal Title
PLoS One
Volume: 7
Issue: 2
Pages: e30946-e30946
DOI
Related Report
Peer Reviewed
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[Journal Article] Gut microbiota of healthy and malnourished children in bangladesh2011
Author(s)
Monira S, Nakamura S, Gotoh K, Izutsu K, Watanabe H, Alam NH, Endtz HP, Cravioto A, Ali SI, Nakaya T, Horii T, Iida T, Alam M
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Journal Title
Front Microbiol
Volume: 228
Pages: 1-7
DOI
Related Report
Peer Reviewed
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[Journal Article] Genotypic profile of Streptococcus suis serotype 2 and clinical features of infection in humans, Thailand2011
Author(s)
Kerdsin A, Dejsirilert S, Puangpatra P, Sripakdee S, Chumla K, Boonkerd N, Polwichai P, Tanimura S, Takeuchi D, Nakayama T, Nakamura S, Akeda Y, Gottschalk M, Sawanpanyalert P, Oishi K
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Journal Title
Emerg Infect Dis
Volume: 17
Issue: 5
Pages: 835-842
DOI
Related Report
Peer Reviewed
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