| Project/Area Number |
23659508
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 感染症 / 重症化予測 / バイオマーカー / デング出血熱 / 免疫測定 / 国際情報交換 / 国際感染症学 |
|---|
| Research Abstract |
Dengue hemorrhagic fever, a severe sequela of dengue virus infection is a life-threatening condition unless proper treatments are not applied. In the present study, a commercially-available portable SPR device SPR01 (Optoquest, Ageo, Japan) was used for development of rapid quantitative assay of biomarkers for dengue hemorrhagic fever. Dengue virus non-structural protein NS1 and two chemokines, IP10 and CCL3 (MIP-1 alpha) were selected as candidates for such biomarkers. Quantitative assays for dengue virus NS1, IP10 and CCL3 were successfully designed and applied to plasma samples collected in hospitals in Ho Chi Minh City, Vietnam, for the evaluation of its usefulness.
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