Development of new control methods for Serratia infections using the lytic enzyme of bacteriophage
| Project/Area Number |
23659524
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUZAKI Shigenobu 高知大学, 教育研究部医療学系, 准教授 (00190439)
DAIBATA Masanori 高知大学, 教育研究部医療学系, 教授 (50263976)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バクテリオファージ / 溶菌酵素 / ファージ療法 / セラチア菌 / グラム陰性菌 / 外膜 |
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| Research Abstract |
Development of multidrug-resistance makes medical treatment to Serratia marscecens infections by antibiotics difficult. We examined a possible control method to S. marscecens infections using the lytic enzyme of bacteriophage (phage). The complete genome sequences of two Serratia phages KSP90 and KSP100, isolated by us, were analyzed. The genes with lytic enzyme domains, however, were not specified. Alternatively, the lytic enzyme of phage ETA was fused with the possible outer membrane penetration domain of the Bacillus phage lytic enzyme. Examination of bacteriolytic activity of the fused protein to S. marscecens is advanced.
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Report
(4 results)
Research Products
(69 results)
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[Journal Article] In silico analysis of AHJD-like viruses, Staphylococcus aureus phages S24-1 and S13', and study of phage S24-1 adsorption.2014
Author(s)
Uchiyama J, Takemura-Uchiyama I, Kato S, Sato M, Ujihara T, Matsui H, Hanaki H, Daibata M, Matsuzaki S.
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Journal Title
Microbiologyopen.
Volume: 3
Issue: 2
Pages: 257-270
DOI
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Peer Reviewed / Open Access
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[Journal Article] Pseudomonas aeruginosa keratitis in mice : effects of topical bacteriophage KPP12 administration2012
Author(s)
Fukuda K, Ishida W, Uchiyama J, Rashel M, Kato S, Morita T, Muraoka A, Sumi T, Matsuzaki S, Daibata M, Fukus hima A
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Pseudomonas aeruginosa keratitis in mice: effects of topical bacteriophage KPP12 administration.2012
Author(s)
Fukuda K, Ishida W, Uchiyama J, Rashel M, Kato S, Morita T, Muraoka A, Sumi T, Matsuzaki S, Daibata M, Fukushima A
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Journal Title
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Peer Reviewed
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[Presentation] A Novel type of spherical bacteriophge, Helicobacter pylori bacteriophage KHP302013
Author(s)
Uchiyama J, Takeuchi H, Sakagucvhi Y, Gamo K, Kato S, Takemura-Uchiyama I, Ujihara T, Daibata M, Matsuzaki S
Organizer
5Th Congress of Europea Microbiologists (FEMS 2013)
Place of Presentation
Leipzig, Germany
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