| Project/Area Number |
23659526
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
HAMAOKA Kenji 京都府立医科大学, 医学研究科, 教授 (60189602)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OZAWA Seiichiro 京都府立医科大学, 医学研究科, 助教 (40405246)
NAKAMURA Akihiro 京都府立医科大学, 医学研究科, 博士研究員 (50313854)
AKIOKA Shinji 京都府立医科大学, 医学研究科, 講師 (60598093)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 小児循環器学 / 血小板 / マイクロバーティクル / プロテオミクス / 質量分析 / 川崎病 / 臨床プロテオミクス / バイオマーカー探索 |
|---|
| Research Abstract |
We evaluated using blood samples of adult control in order as follows: (1)isolation of blood platelet and production of microparticle in blood, (2)deposit or sediment of protein, (3)digestion in solution or medium, (4)culture of hydrogel nano-particle, (5)nano-analysis with HPL-MS/MS, (6)search of data base and identification of protein. In result, 603 protein groups were identificated by nano-particle concentration and digestion in solution.In this study, the rapid and simple protocol for analysis of characteristics of PMP protein in blood sample.
|
