Development of preventative measures against epilepsy using novel model animals (kick-in)
Project/Area Number |
23659529
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Pediatrics
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Research Institution | Fukuoka University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
TAKANO Yukio 福岡大学, 薬学部, 教授 (50113246)
SAITO Ryo 福岡大学, 薬学部, 講師 (80122696)
KATSURABAYASHI Shutaro 福岡大学, 薬学部, 助教 (50435145)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | モデル動物 / フロセミド / てんかん |
Research Abstract |
Dravet syndrome results from various mutations of SCN1A, the gene encoding a1 subunit of Nav1.1 channel. Microdeletion of chromosome including SCN1A leads the most severe phenotype of Dravet syndrome. We have successfully genetically engineered mice bearing a heterozygous microdeletion of Scn1a, the mouse homologue of human SCN1A. The mice exhibited severe convulsions and epileptic discharges on Electro corticogram during 4 to 8 weeks of age. We are now examining effects of NKCC1 inhibitors on the development of the phenotypes whether the inhibitors can exert a prophylactic effect. Also, we are examining the neuro-electrophysiological characteristics of the mice using the slice patch clump method on brain. Primary culture of neurons of the mice are also has been implemented.(金額単位:円)直接経費 間接経費 合 計
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Identification of ATP1A3 mutations by exome sequencing as the cause of alternating hemiplegia of childhood in Japanese patients.2013
Author(s)
Ishii A, Saito Y, Mitsui J, Ishiura H, Yoshimura J, Arai H, Yamashita S, Kimura S, Oguni H, Morishita S, Tsuji S, Sasaki M, Hirose S.
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Journal Title
PLoS One
Volume: 8
Issue: 2
Pages: e56120-e56120
DOI
Related Report
Peer Reviewed
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[Journal Article] Mutations in PRRT2 responsible for paroxysmal kinesigenic dyskinesias also cause benign familialinfantile convulsions.2012
Author(s)
Ono S, Yoshiura K , KinoshitaA, Kikuchi T, Nakane Y, Kato N,Sadamatsu M , Konishi T , Nagamitsu S , Matsuura M, Yasuda A, KomineM, Kanai K , Inoue T , Osamura T , Saito K , Hirose S, Koide H, Tomita H , Ozawa H , Niikawa N and Kurotaki N.
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Journal Title
J Hum Genet
Volume: 57
Issue: 5
Pages: 1-4
DOI
Related Report
Peer Reviewed
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[Journal Article] Diagnosing nocturnal frontal lobe epilepsy: A case study of two children2011
Author(s)
Tomonoh Y, Yasumoto S, Ihara Y, Fujita T, Nakamura N, Ninomiya S, Kodama R, Ideguchi H, Inoue T, Mitsudome A, Hirose S
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Journal Title
Seizure
Volume: 20(7)
Issue: 7
Pages: 583-5
DOI
Related Report
Peer Reviewed
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[Presentation] Genetic analysis of PRRT2 for benign infantile epilepsy, infantile convulsions with choleoathetosis syndrom,and benign convulsions with mild gastroenteritis2012
Author(s)
Ishii A,Yasumoto S,Ihara Y, Inoue T,Fujita T,Nakamura N,Ohfu M,Lee WT,Kaneko S, Hirose S.
Organizer
2012Korea Epilepsy congress
Place of Presentation
Incheon,Korea
Year and Date
2012-06-08
Related Report
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[Presentation] Genetic analysis of PRRT2 for benign infantile epilepsy,infantile convulsions with choleoathetosis syndrom, and benign convulsions with mild gastroenteritis
Author(s)
Ishii A, Yasumoto S, Ihara Y, Inoue T, Fujita T, Nakamura N, Ohfu M, Lee WT, Kaneko S, Hirose S.
Organizer
2012 Korea Epilepsy Congress
Place of Presentation
Incheon, Korea
Related Report
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