An application of imaging mass spectrometry to human placentas

• Project/Area Number: 23659534
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: ITOH Hiroaki 浜松医科大学, 医学部附属病院, 教授 (70263085)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazunao 浜松医科大学, 医学部附属病院, 講師 (50456571)
• Co-Investigator(Renkei-kenkyūsha): HAYASAKA Takahiro 浜松医科大学, 分子イメージング先端研究センター, 助教 (90415927)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 胎児医学 / 成人病胎児起源説 / 質量分析法 / 質量顕微鏡法 / 胎盤 / 妊娠 / 絨毛 / 生体高分子 / 質量分析 / 質量顕微鏡

Research Abstract

A matrix-assisted laser desorption ionization (MALDI)-based mass spectrometer provides clear two-dimensional molecular identification with highly sensitive mass spectrometry from mixtures of ions generated on tissue surfaces. We applied this technology to the molecular identification of phospholipids in the human term placenta and found that sphingomyelin (d18:1/16:0) and phosphatidylcholine (16:0/20:4) were distributed differently between stem and terminal villi. We further identified four phospholipids overexpressed in the terminal villi complicated with severe preeclampsia. Further study is ongoing.

Research Products

• [Journal Article] Prevalence of inappropriate dietary supplement use among pregnant women in Japan (2013)
  • Author(s): Sato Y, Nakanishi T, Chiba T, Yokotani K, Ishinaga K, Takimoto H, Itoh H, Umegaki K
  • Journal Title: Asia Pac J Clin Nutr Volume: 22(1) Pages: 83-89
  • Peer Reviewed
• [Journal Article] Possible contribution of fetal size and gestational age to myocardial tissue Doppler velocities in preterm fetuses (2013)
  • Author(s): K. Sekii, H. Itoh, T. Ogata, S. Iwashima
  • Journal Title: Eur. J. Obstet. Gynecol. Reprod. Biol. Volume: 167 Issue: 1 Pages: 121-121
  • DOI: 10.1016/j.ejogrb.2012.09.027
  • Peer Reviewed
• [Journal Article] Reduction in Levels of Maternal Complement during Delivery by Cesarean Section (2012)
  • Author(s): Kato M, Itoh H, Nagahashi K, Izima M, Hirai K, Tamura M, Uchida T, Yaguchi C, Suzuki K, Sugihara K, Kanayama K.
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 38 Issue: 1 Pages: 165-171
  • DOI: 10.1111/j.1447-0756.2011.01661.x
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord (2012)
  • Author(s): Naomi Fruta
  • Journal Title: Placenta Volume: 33 Issue: 1 Pages: 24-30
  • DOI: 10.1016/j.placenta.2011.10.007
  • Peer Reviewed
• [Journal Article] Successful living donor liver transplantation for fulminant hepatic failure that manifested immediately after cesarean delivery (2012)
  • Author(s): Horikoshi Y, Itoh H, Kikuchi s, Uchida T, Kazunao Suzuki K, Sugihara K, Kanayama N, Mori A, Uemoto S
  • Journal Title: ASAIO Journal Volume: 58 Issue: 2 Pages: 174-186
  • DOI: 10.1097/mat.0b013e3182444ed4
  • Peer Reviewed
• [Journal Article] Fetal myocardial tissue Doppler indices before birth physiologically change in proportion to body size adjusted for gestational age in low-risk term pregnancies (2012)
  • Author(s): Sekii K, Takamichi Ishikawa T, Ogata T, Itoh H, Iwashima S.
  • Journal Title: Early Human Development Volume: (In Press) Issue: 7 Pages: 517-523
  • DOI: 10.1016/j.earlhumdev.2011.12.014
  • Peer Reviewed
• [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation (2012)
  • Author(s): Suzukin K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
  • Journal Title: Clin Exp Obstet Gynecol Volume: XXXIX(3) Pages: 293-298
  • Peer Reviewed
• [Journal Article] Deterioration of myocardial tissue Doppler indices in a case of fetal hydrothorax as a promising indication for clinical intervention before the development of nonimmune hydrops fetalis. (2012)
  • Author(s): K. Sekii, H. Itoh, T. Ogata, S. Iwashima.
  • Journal Title: Arch. Gynecol. Obstet. Volume: 286 Issue: 4 Pages: 1079-1080
  • DOI: 10.1007/s00404-012-2375-6
  • Peer Reviewed
• [Journal Article] Cerebral oxygen saturation evaluated by near-infrared time-resolved spectroscopy (TRS) in pregnant women during caesarean section - a promising new method of maternal monitoring (2012)
  • Author(s): Kaori Yamazaki
  • Journal Title: Clin Physiol Funct Imaging Volume: 32 Issue: 2 Pages: 109-116
  • DOI: 10.1111/cpf.12001
  • Peer Reviewed
• [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation. (2012)
  • Author(s): Suzukin K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
  • Journal Title: Clin Exp Obstet Gynecol Volume: XXXIX(3) Pages: 517-523
  • Peer Reviewed
• [Journal Article] Prevalence of inappropriate dietary supplement use among pregnant women in Japan. (2012)
  • Author(s): Sato Y, Nakanishi T, Chiba T, Yokotani K, Ishinaga K, Takimoto H, Itoh H, Umegaki K.
  • Journal Title: Asia Pac J Clin Nutr Volume: 22 Pages: 83-89
  • Peer Reviewed
• [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation. (2012)
  • Author(s): Suzuki K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
  • Journal Title: Clin Exp Obstet Gynecol Volume: In press
  • Peer Reviewed
• [Journal Article] Fetal myocardial tissue Doppler indices before birth physiologically change in proportion to body size adjusted for gestational age in low-risk term pregnancies. (2012)
  • Author(s): Sekii K, Takamichi Ishikawa T, Ogata T, Itoh H, Iwashima S.
  • Journal Title: Early Hum Dev Volume: In press
  • Peer Reviewed
• [Journal Article] Neonatal exposure to leptin reduces glucose tolerance in adult mice (2011)
  • Author(s): Itoh H, Yura S, Sagawa N, Kanayama N, Konihi I
  • Journal Title: Acta Physiol (Oxf) Volume: 202(2) Issue: 2 Pages: 159-164
  • DOI: 10.1111/j.1748-1716.2011.02268.x
  • Peer Reviewed
• [Journal Article] Branched-chain amino acid supplemented diet during maternal food restriction prevents developmental hypertension in adult rat offspring (2011)
  • Author(s): Fuji T, Yura S, Tatsumi K, Mogami H, Fujita K, Kondoh E, Kakui K, Aoe S, Itoh H, Sagawa N, Fujii S, Konishi I
  • Journal Title: Journal of Developmental Origins of Health and Disease Volume: 2 Issue: 03 Pages: 176-183
  • DOI: 10.1017/s204017441100002x
  • Peer Reviewed
• [Journal Article] The HBC Study Team. Age-specific 3-month cumulative incidence of postpartum depression : The Hamamatsu Birth Cohort (HBC) Study (2011)
  • Author(s): Matsumoto K, Tsuchiya KJ, Itoh H, Kanayama N, Suda S, Matsuzaki H, Iwata Y, Suzuki K, Nakamura K, Mori N, Takei N; The HBC Study Team
  • Journal Title: Journal of Affective Disorders Volume: 133 Issue: 3 Pages: 607-610
  • DOI: 10.1016/j.jad.2011.04.024
  • Peer Reviewed
• [Journal Article] A rapid activated protein C sensitivity test as a diagnostic marker for a suspected venous thromboembolism in pregnancy and puerperium (2011)
  • Author(s): Hirai K, Sugimura M, Ohashi R, Suzuki K, Itoh H, Sugihara K, Kanayama N
  • Journal Title: Gynecologic and Obstetric Investigation Volume: 72 Issue: 1 Pages: 55-62
  • DOI: 10.1159/000322880
  • Peer Reviewed
• [Journal Article] A case of spontaneous rupture of a uterine superficial varicose vein in midgestation. (2011)
  • Author(s): Nakaya Y, Itoh H, Muramatsu K, Otome M, Kobayashi Y, Hirai K, Uchida T, Suzuki K, Sugihara K, and Kanayama N.
  • Journal Title: J Obstet Gynaecol Res Volume: 37 Issue: 8 Pages: 1149-1153
  • DOI: 10.1111/j.1447-0756.2010.01489.x
  • Peer Reviewed
• [Journal Article] Japan Society of Obstetrics and Gynecology ; Japan Association of Obstetricians and Gynecologists. Guidelines for obstetrical practice in Japan : Japan Society of Obstetrics and Gynecology(JSOG) and Japan Association of Obstetricians and Gynecologists(JAO (2011)
  • Author(s): Minakami H, Hiramatsu Y, Koresawa M, Fujii T, Hamada H, Iitsuka Y, Ikeda T, Ishikawa H, Ishimoto H, Itoh H, Kanayama N, Kasuga Y, Kawabata M, Konishi I, Matsubara S, Matsuda H, Murakoshi T, Ohkuchi A, Okai T, Saito S, Sakai M, Satoh S, Sekizawa A, Suzuki
  • Journal Title: J Obstet Gynaecol Res Volume: 37(9) Issue: 9 Pages: 1174-97
  • DOI: 10.1111/j.1447-0756.2011.01653.x
  • Peer Reviewed
• [Journal Article] PIH妊婦の栄養管理/特集 今、改めて妊娠高血圧症候群を考える (2011)
  • Author(s): 伊東宏晃
  • Journal Title: 産婦治療 Volume: 102 Pages: 839-845
  • NAID: 40018836395
• [Journal Article] 妊娠糖尿病と胎児/母体と胎児の栄養学 (2011)
  • Author(s): 伊東宏晃
  • Journal Title: 臨床婦人科産科 Volume: 65 Pages: 676-680
• [Journal Article] 妊婦糖尿病と胎児の発育異常 (2011)
  • Author(s): 伊東宏晃
  • Journal Title: 周産期医学 Volume: 41(12) Pages: 1605-1608
• [Presentation] Comparison of phospholipid molecualr species of human placenta between preeclampsia and normal case by Imaging Mass Spectrometry (2013)
  • Author(s): Yamazaki K, Hayasaka T, Muramatsu K, Nagahashi K, Yaguchi C, Itoh H, Kanayam N
  • Organizer: 60th Annual Meeting of Society for Gynecologic Investigation (SGI).
  • Place of Presentation: Orland, USA
• [Presentation] The analysis of 303 cases with massive postpartum hemorrhage. (2012)
  • Author(s): Tamura N, Itoh H, Uchida T, Suzuki S, Kanayama N.
  • Organizer: 59th Annual Meeting of Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Infantile growth after undernourishment in utero positively correlates with fat deposit in the liver of adult mice, as a risk of Non-alcoholic Fatty Liver Disease (NAFLD). (2012)
  • Author(s): Muramatsu K, Itoh H, Kohmura Y, Yamazaki K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki S, Kanayama N.
  • Organizer: 59th Annual Meeting of Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Real time cerebral hemodynamics evalution by Time-Resolved Spectroscopy (TRS-20) during cesarean operation. (2012)
  • Author(s): Yamazaki K, Itoh H, Muramatsu K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki K, Kanayam N.
  • Organizer: 59th Annual Meeting of Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Infantile growth after undernourishment in utero positively correlates with chronic inflammatory reactions in the white adipose tissues of adult mice, as a risk of developing obesity. (2012)
  • Author(s): Itoh H, Kohmura Y, Muramatsu K, Yamazaki K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki S, Kanayama N.
  • Organizer: 59th Annual Meeting of Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Successful management of deliveries of a woman with PAI-1 deficiency -A critical role of PAI-1 in the maintenance of pregnancy-. (2012)
  • Author(s): Nagahashi K, Iwaki T, Muramatsu K, Yamazaki K, Nakamura Y, Tamura N, Itoh H, Kanayama N.
  • Organizer: 59th Annual Meeting of Society for Gynecologic Investigation (SGI)
  • Place of Presentation: San Diego, USA
• [Presentation] Developmental Origins of Cardiac Remodeling. (2011)
  • Author(s): Itoh H
  • Organizer: Satellite Symposium on 7th World Congress on Developmental Origins of Health and Disease (DOHaD)(招待講演)
  • Place of Presentation: Portland, OR USA.
• [Presentation] Neonatal Growth Rate after Undernourishment in utero Positively Correlates with Chronic Inflammatory Reactions in the White Adipose Tissues of Adult Mice, as a Risk of Developing Obesity. (2011)
  • Author(s): Itoh H, Kohmura KY, Muramatsu K, Uchida T, Suzuki K, Kanayama N.
  • Organizer: 7th World Congress on Developmental Origins of Health and Disease (DOHaD)
  • Place of Presentation: Portland, OR USA.
• [Presentation] 子宮内環境の制御機構ならびに児の長期予後への影響の研究―成人病発症リスクとしての子宮内環境因子の解析をめざして―
  • Author(s): 伊東宏晃
  • Organizer: 神澤医学研究指嚢財団 第13回講演会 神澤医学賞受賞講演(招待講演)
  • Place of Presentation: ホテルオークラ(東京都 港区）
• [Presentation] 低出生体重の長期予後 ―マウス動物モデルの解析―
  • Author(s): 伊東宏晃
  • Organizer: キッセイ薬品工業 中央研究所 特別講演(招待講演)
  • Place of Presentation: キッセイ薬品中央研究所(長野県安曇野市)
• [Presentation] 妊娠高血圧症候群と我が国における妊婦の栄養管理
  • Author(s): 伊東宏晃
  • Organizer: 第32回日本妊娠高血圧学会 シンポジウム(招待講演)
  • Place of Presentation: 金沢歌劇場（石川県金沢市）
• [Presentation] 我が国における妊婦の栄養管理をめぐる諸問題
  • Author(s): 伊東宏晃
  • Organizer: 第18回北海道周産期研修会(招待講演)
  • Place of Presentation: 北海道
• [Book] 合併症妊娠 改訂３版 村田雄二編 (2011)
  • Author(s): 伊東宏晃
  • Publisher: メディカ出版
• [Book] 周産期医学必修知識 第7版 (2011)
  • Author(s): 伊東宏晃
  • Publisher: 東京医学社