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An application of imaging mass spectrometry to human placentas

Research Project

Project/Area Number 23659534
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Embryonic/Neonatal medicine
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

ITOH Hiroaki  浜松医科大学, 医学部附属病院, 教授 (70263085)

Co-Investigator(Kenkyū-buntansha) SUZUKI Kazunao  浜松医科大学, 医学部附属病院, 講師 (50456571)
Co-Investigator(Renkei-kenkyūsha) HAYASAKA Takahiro  浜松医科大学, 分子イメージング先端研究センター, 助教 (90415927)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords胎児医学 / 成人病胎児起源説 / 質量分析法 / 質量顕微鏡法 / 胎盤 / 妊娠 / 絨毛 / 生体高分子 / 質量分析 / 質量顕微鏡
Research Abstract

A matrix-assisted laser desorption ionization (MALDI)-based mass spectrometer provides clear two-dimensional molecular identification with highly sensitive mass spectrometry from mixtures of ions generated on tissue surfaces. We applied this technology to the molecular identification of phospholipids in the human term placenta and found that sphingomyelin (d18:1/16:0) and phosphatidylcholine (16:0/20:4) were distributed differently between stem and terminal villi. We further identified four phospholipids overexpressed in the terminal villi complicated with severe preeclampsia. Further study is ongoing.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (36 results)

All 2013 2012 2011 Other

All Journal Article (22 results) (of which Peer Reviewed: 19 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Prevalence of inappropriate dietary supplement use among pregnant women in Japan2013

    • Author(s)
      Sato Y, Nakanishi T, Chiba T, Yokotani K, Ishinaga K, Takimoto H, Itoh H, Umegaki K
    • Journal Title

      Asia Pac J Clin Nutr

      Volume: 22(1) Pages: 83-89

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible contribution of fetal size and gestational age to myocardial tissue Doppler velocities in preterm fetuses2013

    • Author(s)
      K. Sekii, H. Itoh, T. Ogata, S. Iwashima
    • Journal Title

      Eur. J. Obstet. Gynecol. Reprod. Biol.

      Volume: 167 Issue: 1 Pages: 121-121

    • DOI

      10.1016/j.ejogrb.2012.09.027

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduction in Levels of Maternal Complement during Delivery by Cesarean Section2012

    • Author(s)
      Kato M, Itoh H, Nagahashi K, Izima M, Hirai K, Tamura M, Uchida T, Yaguchi C, Suzuki K, Sugihara K, Kanayama K.
    • Journal Title

      Journal of Obstetrics and Gynaecology Research

      Volume: 38 Issue: 1 Pages: 165-171

    • DOI

      10.1111/j.1447-0756.2011.01661.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical detection of meconium in the fetal membrane, placenta and umbilical cord2012

    • Author(s)
      Naomi Fruta
    • Journal Title

      Placenta

      Volume: 33 Issue: 1 Pages: 24-30

    • DOI

      10.1016/j.placenta.2011.10.007

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Successful living donor liver transplantation for fulminant hepatic failure that manifested immediately after cesarean delivery2012

    • Author(s)
      Horikoshi Y, Itoh H, Kikuchi s, Uchida T, Kazunao Suzuki K, Sugihara K, Kanayama N, Mori A, Uemoto S
    • Journal Title

      ASAIO Journal

      Volume: 58 Issue: 2 Pages: 174-186

    • DOI

      10.1097/mat.0b013e3182444ed4

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fetal myocardial tissue Doppler indices before birth physiologically change in proportion to body size adjusted for gestational age in low-risk term pregnancies2012

    • Author(s)
      Sekii K, Takamichi Ishikawa T, Ogata T, Itoh H, Iwashima S.
    • Journal Title

      Early Human Development

      Volume: (In Press) Issue: 7 Pages: 517-523

    • DOI

      10.1016/j.earlhumdev.2011.12.014

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation2012

    • Author(s)
      Suzukin K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
    • Journal Title

      Clin Exp Obstet Gynecol

      Volume: XXXIX(3) Pages: 293-298

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Deterioration of myocardial tissue Doppler indices in a case of fetal hydrothorax as a promising indication for clinical intervention before the development of nonimmune hydrops fetalis.2012

    • Author(s)
      K. Sekii, H. Itoh, T. Ogata, S. Iwashima.
    • Journal Title

      Arch. Gynecol. Obstet.

      Volume: 286 Issue: 4 Pages: 1079-1080

    • DOI

      10.1007/s00404-012-2375-6

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cerebral oxygen saturation evaluated by near-infrared time-resolved spectroscopy (TRS) in pregnant women during caesarean section - a promising new method of maternal monitoring2012

    • Author(s)
      Kaori Yamazaki
    • Journal Title

      Clin Physiol Funct Imaging

      Volume: 32 Issue: 2 Pages: 109-116

    • DOI

      10.1111/cpf.12001

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation.2012

    • Author(s)
      Suzukin K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
    • Journal Title

      Clin Exp Obstet Gynecol

      Volume: XXXIX(3) Pages: 517-523

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of inappropriate dietary supplement use among pregnant women in Japan.2012

    • Author(s)
      Sato Y, Nakanishi T, Chiba T, Yokotani K, Ishinaga K, Takimoto H, Itoh H, Umegaki K.
    • Journal Title

      Asia Pac J Clin Nutr

      Volume: 22 Pages: 83-89

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Feto-Umbilical blood flow obstruction increases placental tissue oxygenation.2012

    • Author(s)
      Suzuki K, Itoh H, Muramatsu K, Yamazaki K, Furuta N, Nagahashi K, Tamura N, Kato M, Uchida U, Sugihara K, Sumimoto K, Kanayama N.
    • Journal Title

      Clin Exp Obstet Gynecol

      Volume: In press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fetal myocardial tissue Doppler indices before birth physiologically change in proportion to body size adjusted for gestational age in low-risk term pregnancies.2012

    • Author(s)
      Sekii K, Takamichi Ishikawa T, Ogata T, Itoh H, Iwashima S.
    • Journal Title

      Early Hum Dev

      Volume: In press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neonatal exposure to leptin reduces glucose tolerance in adult mice2011

    • Author(s)
      Itoh H, Yura S, Sagawa N, Kanayama N, Konihi I
    • Journal Title

      Acta Physiol (Oxf)

      Volume: 202(2) Issue: 2 Pages: 159-164

    • DOI

      10.1111/j.1748-1716.2011.02268.x

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Branched-chain amino acid supplemented diet during maternal food restriction prevents developmental hypertension in adult rat offspring2011

    • Author(s)
      Fuji T, Yura S, Tatsumi K, Mogami H, Fujita K, Kondoh E, Kakui K, Aoe S, Itoh H, Sagawa N, Fujii S, Konishi I
    • Journal Title

      Journal of Developmental Origins of Health and Disease

      Volume: 2 Issue: 03 Pages: 176-183

    • DOI

      10.1017/s204017441100002x

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The HBC Study Team. Age-specific 3-month cumulative incidence of postpartum depression : The Hamamatsu Birth Cohort (HBC) Study2011

    • Author(s)
      Matsumoto K, Tsuchiya KJ, Itoh H, Kanayama N, Suda S, Matsuzaki H, Iwata Y, Suzuki K, Nakamura K, Mori N, Takei N; The HBC Study Team
    • Journal Title

      Journal of Affective Disorders

      Volume: 133 Issue: 3 Pages: 607-610

    • DOI

      10.1016/j.jad.2011.04.024

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A rapid activated protein C sensitivity test as a diagnostic marker for a suspected venous thromboembolism in pregnancy and puerperium2011

    • Author(s)
      Hirai K, Sugimura M, Ohashi R, Suzuki K, Itoh H, Sugihara K, Kanayama N
    • Journal Title

      Gynecologic and Obstetric Investigation

      Volume: 72 Issue: 1 Pages: 55-62

    • DOI

      10.1159/000322880

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A case of spontaneous rupture of a uterine superficial varicose vein in midgestation.2011

    • Author(s)
      Nakaya Y, Itoh H, Muramatsu K, Otome M, Kobayashi Y, Hirai K, Uchida T, Suzuki K, Sugihara K, and Kanayama N.
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 37 Issue: 8 Pages: 1149-1153

    • DOI

      10.1111/j.1447-0756.2010.01489.x

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Japan Society of Obstetrics and Gynecology ; Japan Association of Obstetricians and Gynecologists. Guidelines for obstetrical practice in Japan : Japan Society of Obstetrics and Gynecology(JSOG) and Japan Association of Obstetricians and Gynecologists(JAO2011

    • Author(s)
      Minakami H, Hiramatsu Y, Koresawa M, Fujii T, Hamada H, Iitsuka Y, Ikeda T, Ishikawa H, Ishimoto H, Itoh H, Kanayama N, Kasuga Y, Kawabata M, Konishi I, Matsubara S, Matsuda H, Murakoshi T, Ohkuchi A, Okai T, Saito S, Sakai M, Satoh S, Sekizawa A, Suzuki
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 37(9) Issue: 9 Pages: 1174-97

    • DOI

      10.1111/j.1447-0756.2011.01653.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] PIH妊婦の栄養管理/特集 今、改めて妊娠高血圧症候群を考える2011

    • Author(s)
      伊東宏晃
    • Journal Title

      産婦治療

      Volume: 102 Pages: 839-845

    • NAID

      40018836395

    • Related Report
      2011 Research-status Report
  • [Journal Article] 妊娠糖尿病と胎児/母体と胎児の栄養学2011

    • Author(s)
      伊東宏晃
    • Journal Title

      臨床婦人科産科

      Volume: 65 Pages: 676-680

    • Related Report
      2011 Research-status Report
  • [Journal Article] 妊婦糖尿病と胎児の発育異常2011

    • Author(s)
      伊東宏晃
    • Journal Title

      周産期医学

      Volume: 41(12) Pages: 1605-1608

    • Related Report
      2011 Research-status Report
  • [Presentation] Comparison of phospholipid molecualr species of human placenta between preeclampsia and normal case by Imaging Mass Spectrometry2013

    • Author(s)
      Yamazaki K, Hayasaka T, Muramatsu K, Nagahashi K, Yaguchi C, Itoh H, Kanayam N
    • Organizer
      60th Annual Meeting of Society for Gynecologic Investigation (SGI).
    • Place of Presentation
      Orland, USA
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] The analysis of 303 cases with massive postpartum hemorrhage.2012

    • Author(s)
      Tamura N, Itoh H, Uchida T, Suzuki S, Kanayama N.
    • Organizer
      59th Annual Meeting of Society for Gynecologic Investigation (SGI)
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Infantile growth after undernourishment in utero positively correlates with fat deposit in the liver of adult mice, as a risk of Non-alcoholic Fatty Liver Disease (NAFLD).2012

    • Author(s)
      Muramatsu K, Itoh H, Kohmura Y, Yamazaki K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki S, Kanayama N.
    • Organizer
      59th Annual Meeting of Society for Gynecologic Investigation (SGI)
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Real time cerebral hemodynamics evalution by Time-Resolved Spectroscopy (TRS-20) during cesarean operation.2012

    • Author(s)
      Yamazaki K, Itoh H, Muramatsu K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki K, Kanayam N.
    • Organizer
      59th Annual Meeting of Society for Gynecologic Investigation (SGI)
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Infantile growth after undernourishment in utero positively correlates with chronic inflammatory reactions in the white adipose tissues of adult mice, as a risk of developing obesity.2012

    • Author(s)
      Itoh H, Kohmura Y, Muramatsu K, Yamazaki K, Nagahashi K, Nakamura Y, Tamura N, Uchida T, Suzuki S, Kanayama N.
    • Organizer
      59th Annual Meeting of Society for Gynecologic Investigation (SGI)
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Successful management of deliveries of a woman with PAI-1 deficiency -A critical role of PAI-1 in the maintenance of pregnancy-.2012

    • Author(s)
      Nagahashi K, Iwaki T, Muramatsu K, Yamazaki K, Nakamura Y, Tamura N, Itoh H, Kanayama N.
    • Organizer
      59th Annual Meeting of Society for Gynecologic Investigation (SGI)
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Developmental Origins of Cardiac Remodeling.2011

    • Author(s)
      Itoh H
    • Organizer
      Satellite Symposium on 7th World Congress on Developmental Origins of Health and Disease (DOHaD)(招待講演)
    • Place of Presentation
      Portland, OR USA.
    • Related Report
      2011 Research-status Report
  • [Presentation] Neonatal Growth Rate after Undernourishment in utero Positively Correlates with Chronic Inflammatory Reactions in the White Adipose Tissues of Adult Mice, as a Risk of Developing Obesity.2011

    • Author(s)
      Itoh H, Kohmura KY, Muramatsu K, Uchida T, Suzuki K, Kanayama N.
    • Organizer
      7th World Congress on Developmental Origins of Health and Disease (DOHaD)
    • Place of Presentation
      Portland, OR USA.
    • Related Report
      2011 Research-status Report
  • [Presentation] 子宮内環境の制御機構ならびに児の長期予後への影響の研究―成人病発症リスクとしての子宮内環境因子の解析をめざして―

    • Author(s)
      伊東宏晃
    • Organizer
      神澤医学研究指嚢財団 第13回講演会 神澤医学賞受賞講演(招待講演)
    • Place of Presentation
      ホテルオークラ(東京都 港区)
    • Related Report
      2011 Research-status Report
  • [Presentation] 低出生体重の長期予後 ―マウス動物モデルの解析―

    • Author(s)
      伊東宏晃
    • Organizer
      キッセイ薬品工業 中央研究所 特別講演(招待講演)
    • Place of Presentation
      キッセイ薬品中央研究所(長野県安曇野市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 妊娠高血圧症候群と我が国における妊婦の栄養管理

    • Author(s)
      伊東宏晃
    • Organizer
      第32回日本妊娠高血圧学会 シンポジウム(招待講演)
    • Place of Presentation
      金沢歌劇場(石川県金沢市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 我が国における妊婦の栄養管理をめぐる諸問題

    • Author(s)
      伊東宏晃
    • Organizer
      第18回北海道周産期研修会(招待講演)
    • Place of Presentation
      北海道
    • Related Report
      2011 Research-status Report
  • [Book] 合併症妊娠 改訂3版 村田雄二編2011

    • Author(s)
      伊東宏晃
    • Publisher
      メディカ出版
    • Related Report
      2011 Research-status Report
  • [Book] 周産期医学必修知識 第7版2011

    • Author(s)
      伊東宏晃
    • Publisher
      東京医学社
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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