• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of stem cell therapy for epidermolysis bullosa model mice expressing humanized dermo-epidermal junctional protein

Research Project

Project/Area Number 23659539
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionHokkaido University

Principal Investigator

SHIMIZU Hiroshi  北海道大学, 大学院・医学研究科, 教授 (00146672)

Co-Investigator(Kenkyū-buntansha) ABE Riichiro  北海道大学, 大学院・医学研究科, 准教授 (60344511)
FUJITA Yasuyuki  北海道大学, 北海道大学病院, 助教 (80374437)
Project Period (FY) 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords皮膚炎症・再生学 / 表皮水疱症 / VII型コラーゲン / 骨髄移植 / 骨髄由来細胞 / transdifferentiation
Research Abstract

The aim of the project is to investigate the effect of stem cell therapy for congenital severe skin disorder, recessive dystrophic epidermolysis bullosa(EB), using protein-humanized EB model mice. We performed bone marrow transplantation from GFP Tg mice to EB model mice lacking murine type VII collagen(COL7) and expressing incomplete/complete human COL7. Epithelized skin areas showed GFP+bone marrow-derived keratinocytes as many as 0. 2%. Murine COL7 mRNA and COL7 protein were also expressed in some mice, but the expression levels were low. We are now performing and analyzing another bone marrow transplantation model using HMGB1, CCL27 and CCL19, which are known to enhance transdifferentiation and recruitment from bone marrow cells to skin component cells.

Report

(2 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (4 results)

All 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Conversion from human haematopoietic stem cells to keratinocytes requires keratinocyte secretory factors2012

    • Author(s)
      Fujita Y, Inokuma D, et al.
    • Journal Title

      Clinical and Experimental Dermatology

      Volume: (印刷中) Issue: 6 Pages: 658-664

    • DOI

      10.1111/j.1365-2230.2011.04312.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conversion from human haematopoietic stem cells to keratinocytes requires keratinocyte secretory factors2012

    • Author(s)
      Fujita Y, Inokuma D, et al
    • Journal Title

      Clinical and Experimental Dermatology

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] Exploring stem cell therapies for junctional epidermolysis bullosa model mice2011

    • Author(s)
      Fujita Y, Abe R, Nishie W, Shimizu H.
    • Organizer
      The 22nd World Congress of Dermatology
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2011-05-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Exploring stem cell therapies for junctional epidermolysis bullosa model mice2011

    • Author(s)
      Fujita Y, Abe R, et al
    • Organizer
      The 22nd World Congress of Dermatology
    • Place of Presentation
      COEX Convention and Exhibition Center (Korea)(招待講演)
    • Year and Date
      2011-05-26
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi