| Project/Area Number |
23659541
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Tohoku University |
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Principal Investigator |
AIBA Setsuya 東北大学, 大学院・医学系研究科, 教授 (80159269)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUASHI Masato 東北大学, 病院, 助教 (20400369)
伊藤 由美子 東北大学, 大学病院, 技術一般職員 (00375057)
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| Co-Investigator(Renkei-kenkyūsha) |
ITO Yumiko 東北大学, 病院, 技術一般職員 (00375057)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 皮膚病理学 / 遺伝子発現解析 / 鉄代謝 / 乳房外パジェット病 / レーザーミクロダイセクション / 乾癬 / 円形脱毛症 / サイトカイン / 転写因子 |
|---|
| Research Abstract |
To apply the analysis using laser microdissection to skin diseases, we first compared mRNA expression between the lower and upper epidermis. When we examined filaggrin mRNA expression as a representative gene expressed only in the granular layer, the analysis using laser microdissection clearly demonstrated more abundant expression of filaggrinmRNA in the upper epidermis than in the lower epidermis. Next, we demonstrated augmented expression of mRNA related with the excretion of iron from inside to outside of the cell in the course of keratinization, which corresponds with the iron distribution in the epidermis. Finally, we examined immune-related gene, RANKL mRNA expression in extramammary Paget's disease. We could demonstrated more RANKL mRNA in the epidermis containing Paget's cells than the normal epidermis
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