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Development of the visualization method of the stratum corneum barrier function by Time-of-Flight Secondary Ion Mass Spectroscopy (TOF-SIMS)

Research Project

Project/Area Number 23659555
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionKeio University

Principal Investigator

KUBO Akiharu  慶應義塾大学, 医学部, 講師 (70335256)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords皮膚病退学 / 皮膚生理・生物学 / イメージング / 皮膚バリア / アトピー性皮膚炎 / 質量分析 / 飛行時間型質量分析顕微鏡 / 角質バリア / セラミド
Research Abstract

The stratum corneum (SC), the outermost barrier of mammalian bodies, consists of layers of cornified keratinocytes with intercellular spaces sealed with lipids. The insolubility of the SC has hampered in-depth analysis, and the SC has been considered a ho

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (14 results) (of which Invited: 1 results) Remarks (4 results)

  • [Journal Article] The stratum corneum comprises three layers with distinct metal-ion barrier properties2013

    • Author(s)
      Kubo A, Ishizaki I, Kubo A, Kawasaki H, Nagao K, Ohashi Y, and Amagai M
    • Journal Title

      Sci Rep

      Volume: 3 Pages: 1731-1731

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The stratum corneum comprises three layers with distinct metal-ion barrier properties2013

    • Author(s)
      Kubo A, Ishizaki I, Kubo A, Kawasaki H, Nagao K, Ohashi Y, Amagai M
    • Journal Title

      Sci. Rep

      Volume: 3 Issue: 1 Pages: 1731-1731

    • DOI

      10.1038/srep01731

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 3D Imaging of the Mammalian Epidermis2012

    • Author(s)
      Kubo A
    • Organizer
      37th Annual Meeting of the Japanese Society for Investigative Dermatology
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] The stratum corneum comprises three layers with distinct barrier properties, as revealed by TOF-SIMS imaging.2012

    • Author(s)
      Kubo A, Ishizaki I, Kubo A, Kawasaki H, Ohashi Y, and Amagai M
    • Organizer
      62nd Montagna Symposium on the Biology of Skin 2012
    • Place of Presentation
      Portland, Oregon,USA
    • Related Report
      2012 Final Research Report
  • [Presentation] High-resolution TOF-SIMS Imaging reveals Multi-layered Barrier Structure of the Stratum Corneum of Skin.2012

    • Author(s)
      Kubo A, Ishizaki I, Kubo A, Kawasaki H, Ohashi Y, and Amagai M
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] The stratum corneum comprises three layers with distinct barrier properties, as revealed by TOF- SIMS imaging2012

    • Author(s)
      Kubo A
    • Organizer
      62nd Montagna Symposium on the Biology of Skin 2012
    • Place of Presentation
      Portland, Oregon
    • Related Report
      2012 Annual Research Report
  • [Presentation] High-resolution TOF-SIMS Imaging reveals Multi-layered Barrier Structure of the Stratum Corneum of Skin2012

    • Author(s)
      Kubo A
    • Organizer
      19th International Mass Spectrometry Conference
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 3D Imaging of the Mammalian Epidermis2012

    • Author(s)
      Kubo A
    • Organizer
      37th Annual Meeting of the Japanese society for Investigative Dermatology
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Surface barrier and immunology of the skin.2012

    • Author(s)
      Kubo A
    • Organizer
      Surface Barrier Immunology Study Group (SBARIS) The 4th stage, 3rd meeting(招待講演)
    • Place of Presentation
      大阪:千里阪急ホテル
    • Related Report
      2011 Research-status Report
  • [Presentation] Physiological and immunological barriers in the epidermis2011

    • Author(s)
      Kubo A
    • Organizer
      The 59th Annual Meeting of Japanese Association for Dental Research(招待講演)
    • Place of Presentation
      広島:広島国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] Physiological and immunological barriers in the epidermis2011

    • Author(s)
      Kubo A
    • Organizer
      Asia Pacific Association of Pediatric Allergy, Respirology & Immunology 2011(招待講演)
    • Place of Presentation
      福岡:福岡国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] High Resolution TOF-SIMS Imaging of the Multi-Layered Barrier Structure of Mouse Skin2011

    • Author(s)
      Ishizaki I, Kubo A, Ohashi Y, Yamamoto A, Hammond J, Fisher G, Bryan S
    • Organizer
      American Vaccume Society 58th International Symposimu & Exhibition
    • Place of Presentation
      Nashville, Tennessee, USA: Nashville Convention Center
    • Related Report
      2011 Research-status Report
  • [Presentation] TOF-SIMS Characterization of In Vivo and Transdermally Treated Mouse Skin2011

    • Author(s)
      Ishizaki I, Hammond J, Bryan S, Kubo A, and Yamamoto A
    • Organizer
      18th International Conference on Secondary Ion Mass Spectrometry
    • Place of Presentation
      Riva del Garda, Trentino, Italy: Riva del Garda Fierecongressi SpA
    • Related Report
      2011 Research-status Report
  • [Presentation] High-resolution TOF-SIMS imaging and sequential immunofluorescent analysis of multi-layered barrier structure of the mouse skin stratum corneum2011

    • Author(s)
      Kubo A, Ishizaki I, Kubo A, Kawasaki H, Ohashi Y, and Amagai M
    • Organizer
      59th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Denver, Colorado, USA: Denver Convention Center
    • Related Report
      2011 Research-status Report
  • [Presentation] TOF-SIMSによるマウス皮膚組織の深さ方向分析2011

    • Author(s)
      石崎逸子, Hammond J S, Bryan S R, 久保亮治, 山本公
    • Organizer
      第59回日本質量分析学会
    • Place of Presentation
      大阪:ホテル阪急エキスポパーク
    • Related Report
      2011 Research-status Report
  • [Presentation] High-resolution time-of-flight secondary ion mass spectrometry imaging and sequential immunofluorescent analysis of multi-layered barrier structure of the mouse skin stratum corneum2011

    • Author(s)
      久保亮治, 石崎逸子, 久保亜紀子, 川崎洋, 大橋善治, 天谷雅行
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道:北海道大学
    • Related Report
      2011 Research-status Report
  • [Remarks] 皮膚バリア機構解明プロジェクト

    • URL

      http://www.derma.med.keio.ac.jp/derma/r_research/project7.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 皮膚"バリオロジー":バリア機能異常から見たアレルギー疾患病態解明

    • URL

      http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/kubo/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 皮膚バリア機構解明プロジェクト

    • URL

      http://www.derma.med.keio.ac.jp/derma/r_research/project7.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 皮膚"バリオロジー":バリア機能異常から見たアレルギー疾患病態解明

    • URL

      http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/kubo/index.html

    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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