Development of the visualization method of the stratum corneum barrier function by Time-of-Flight Secondary Ion Mass Spectroscopy (TOF-SIMS)

• Project/Area Number: 23659555
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Keio University
• Principal Investigator: KUBO Akiharu 慶應義塾大学, 医学部, 講師 (70335256)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 皮膚病退学 / 皮膚生理・生物学 / イメージング / 皮膚バリア / アトピー性皮膚炎 / 質量分析 / 飛行時間型質量分析顕微鏡 / 角質バリア / セラミド

Research Abstract

The stratum corneum (SC), the outermost barrier of mammalian bodies, consists of layers of cornified keratinocytes with intercellular spaces sealed with lipids. The insolubility of the SC has hampered in-depth analysis, and the SC has been considered a ho

Research Products

• [Journal Article] The stratum corneum comprises three layers with distinct metal-ion barrier properties (2013)
  • Author(s): Kubo A, Ishizaki I, Kubo A, Kawasaki H, Nagao K, Ohashi Y, and Amagai M
  • Journal Title: Sci Rep Volume: 3 Pages: 1731-1731
  • Peer Reviewed
• [Journal Article] The stratum corneum comprises three layers with distinct metal-ion barrier properties (2013)
  • Author(s): Kubo A, Ishizaki I, Kubo A, Kawasaki H, Nagao K, Ohashi Y, Amagai M
  • Journal Title: Sci. Rep Volume: 3 Issue: 1 Pages: 1731-1731
  • DOI: 10.1038/srep01731
  • Peer Reviewed
• [Presentation] 3D Imaging of the Mammalian Epidermis (2012)
  • Author(s): Kubo A
  • Organizer: 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Okinawa, Japan
• [Presentation] The stratum corneum comprises three layers with distinct barrier properties, as revealed by TOF-SIMS imaging. (2012)
  • Author(s): Kubo A, Ishizaki I, Kubo A, Kawasaki H, Ohashi Y, and Amagai M
  • Organizer: 62nd Montagna Symposium on the Biology of Skin 2012
  • Place of Presentation: Portland, Oregon,USA
• [Presentation] High-resolution TOF-SIMS Imaging reveals Multi-layered Barrier Structure of the Stratum Corneum of Skin. (2012)
  • Author(s): Kubo A, Ishizaki I, Kubo A, Kawasaki H, Ohashi Y, and Amagai M
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: Kyoto, Japan
• [Presentation] The stratum corneum comprises three layers with distinct barrier properties, as revealed by TOF- SIMS imaging (2012)
  • Author(s): Kubo A
  • Organizer: 62nd Montagna Symposium on the Biology of Skin 2012
  • Place of Presentation: Portland, Oregon
• [Presentation] High-resolution TOF-SIMS Imaging reveals Multi-layered Barrier Structure of the Stratum Corneum of Skin (2012)
  • Author(s): Kubo A
  • Organizer: 19th International Mass Spectrometry Conference
  • Place of Presentation: Kyoto, Japan
• [Presentation] 3D Imaging of the Mammalian Epidermis (2012)
  • Author(s): Kubo A
  • Organizer: 37th Annual Meeting of the Japanese society for Investigative Dermatology
  • Place of Presentation: Okinawa, Japan
  • Invited
• [Presentation] Surface barrier and immunology of the skin. (2012)
  • Author(s): Kubo A
  • Organizer: Surface Barrier Immunology Study Group (SBARIS) The 4th stage, 3rd meeting(招待講演)
  • Place of Presentation: 大阪：千里阪急ホテル
• [Presentation] Physiological and immunological barriers in the epidermis (2011)
  • Author(s): Kubo A
  • Organizer: The 59th Annual Meeting of Japanese Association for Dental Research(招待講演)
  • Place of Presentation: 広島：広島国際会議場
• [Presentation] Physiological and immunological barriers in the epidermis (2011)
  • Author(s): Kubo A
  • Organizer: Asia Pacific Association of Pediatric Allergy, Respirology & Immunology 2011(招待講演)
  • Place of Presentation: 福岡：福岡国際会議場
• [Presentation] High Resolution TOF-SIMS Imaging of the Multi-Layered Barrier Structure of Mouse Skin (2011)
  • Author(s): Ishizaki I, Kubo A, Ohashi Y, Yamamoto A, Hammond J, Fisher G, Bryan S
  • Organizer: American Vaccume Society 58th International Symposimu & Exhibition
  • Place of Presentation: Nashville, Tennessee, USA: Nashville Convention Center
• [Presentation] TOF-SIMS Characterization of In Vivo and Transdermally Treated Mouse Skin (2011)
  • Author(s): Ishizaki I, Hammond J, Bryan S, Kubo A, and Yamamoto A
  • Organizer: 18th International Conference on Secondary Ion Mass Spectrometry
  • Place of Presentation: Riva del Garda, Trentino, Italy: Riva del Garda Fierecongressi SpA
• [Presentation] High-resolution TOF-SIMS imaging and sequential immunofluorescent analysis of multi-layered barrier structure of the mouse skin stratum corneum (2011)
  • Author(s): Kubo A, Ishizaki I, Kubo A, Kawasaki H, Ohashi Y, and Amagai M
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, Colorado, USA: Denver Convention Center
• [Presentation] TOF-SIMSによるマウス皮膚組織の深さ方向分析 (2011)
  • Author(s): 石崎逸子, Hammond J S, Bryan S R, 久保亮治, 山本公
  • Organizer: 第59回日本質量分析学会
  • Place of Presentation: 大阪：ホテル阪急エキスポパーク
• [Presentation] High-resolution time-of-flight secondary ion mass spectrometry imaging and sequential immunofluorescent analysis of multi-layered barrier structure of the mouse skin stratum corneum (2011)
  • Author(s): 久保亮治, 石崎逸子, 久保亜紀子, 川崎洋, 大橋善治, 天谷雅行
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道：北海道大学
• [Remarks] 皮膚バリア機構解明プロジェクト
  • URL: http://www.derma.med.keio.ac.jp/derma/r_research/project7.html
• [Remarks] 皮膚"バリオロジー":バリア機能異常から見たアレルギー疾患病態解明
  • URL: http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/kubo/index.html
• [Remarks] 皮膚バリア機構解明プロジェクト
  • URL: http://www.derma.med.keio.ac.jp/derma/r_research/project7.html
• [Remarks] 皮膚"バリオロジー"：バリア機能異常から見たアレルギー疾患病態解明
  • URL: http://www.careerpath-prj.keio.ac.jp/kanrinmaru/scholar/kubo/index.html