Roles of versican in development and maintenance of hair follicles

• Project/Area Number: 23659556
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Aichi Medical University
• Principal Investigator: WATANABE Hideto 愛知医科大学, 付置研究所, 教授 (90240514)
• Co-Investigator(Renkei-kenkyūsha): UTANI Atsushi 長崎大学, 医学部・皮膚科学講座, 教授 (10292707)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: バーシカン / プロテオグリカン / 細胞外マトリックス / ノックアウトマウス / 毛包 / 遺伝子改変マウス / コンドロイチン硫酸 / グリコサミノグリカン

Research Abstract

Versican (Vcan) is a large chondroitin sulfate proteoglycan of the extracellular matrix, which is transiently expressed in mesenchymal condensation areas such as cartilage primordium and dermal papilla. Here, we aimed to investigate the role of Vcan in development and maintenance of hair follicles. Though we performed intradermal injection of adenovirus expressing cre into Vcan<flox/flox> mice, we could not ablate its expression in dermal papilla cells. Currently, we are planning to generate Corin-cre; Vcan<flox/flox> mice, which lack Vcan expression in dermal papillae.

Research Products

• [Journal Article] Alterations in the chondroitin sulfate chain in human osteoarthritic cartilage of the knee (2014)
  • Author(s): Ishimaru D, Sugiura N, Akiyama H, Watanabe H, Matsumoto K
  • Journal Title: Osteoarthritis Cartilage Volume: (in press)
• [Journal Article] Chondroitinase from baculovirus Bombyx mori nucleopolyhedrovirus and chondroitin sulfate from silkworm Bombyx mori. (2013)
  • Author(s): Sugiura, N., Ikeda, M., Shioiri, T., Yoshimura, M., Kobayashi, M. and Watanabe, H.
  • Journal Title: Glycobiology Volume: 23 Issue: 12 Pages: 1520-1530
  • DOI: 10.1093/glycob/cwt082
  • Peer Reviewed
• [Journal Article] Sulfation of the bikunin chondroitin sulfate chain determines heavy chain·hyaluronan complex formation (2013)
  • Author(s): Lord MS, Day AJ, Youssef P, Zhuo L, Watanabe H, Caterson B, Whitelock JM.
  • Journal Title: J Biol Chem. Volume: 288 Issue: 32 Pages: 22930-22941
  • DOI: 10.1074/jbc.m112.404186
  • Peer Reviewed
• [Journal Article] Involvement of heparan sulfate 6-O-sulfation in regulation of energy metabolism and alteration of thyroid hormone levels in male mice. (2013)
  • Author(s): Nagai N, Habuchi H, Sugaya N, Nakamura M, Imamura T, Watanabe H, Kimata K.
  • Journal Title: Glycobiology Volume: 23 Issue: 8 Pages: 980-992
  • DOI: 10.1093/glycob/cwt037
  • Peer Reviewed
• [Journal Article] Construction of a chondroitin sulfate library with defined structures and analysis of molecular interactions. (2012)
  • Author(s): Sugiura N, Shioiri T, Chiba M, Sato T, Narimatsu H, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 287 Issue: 52 Pages: 43390-43400
  • DOI: 10.1074/jbc.m112.412676
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate synthase-2 is necessary for chain extension of chondroitin sulfate but not critical for skeletal development. (2012)
  • Author(s): Ogawa H, Hatano S, Sugiura N, Nagai N, Sato T, Shimizu K, Narimatsu H, Kimata K, Watanabe H
  • Journal Title: PLoS ONE Volume: 7 Issue: 8 Pages: 1-12
  • DOI: 10.1371/journal.pone.0043806
  • Peer Reviewed
• [Journal Article] Versican/PG-M is essential for ventricular septal formation subsequent to cardiac atrioventricular cushion development (2012)
  • Author(s): S. Hatano, K. Kimata, N. Hiraiwa, M. Kusakabe, Z. Isogai, E. Adachi, T. Shinomura, and H. Watanabe
  • Journal Title: Glycobiology Volume: 22 (9) Issue: 9 Pages: 1268-1277
  • DOI: 10.1093/glycob/cws095
  • Peer Reviewed
• [Journal Article] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth. (2012)
  • Author(s): Kono A, Oguri A, Yokoo K, Watanabe H
  • Journal Title: J Plast Surg Hand Surg Volume: 46 Issue: 5 Pages: 308-312
  • DOI: 10.3109/2000656x.2012.696197
• [Journal Article] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth. (2012)
  • Author(s): Kono A
  • Journal Title: J Plast Surg Hand Surg Volume: 46 Pages: 308-312
  • Peer Reviewed
• [Journal Article] Keratan Sulfate Restricts Neural Plasticity after Spinal Cord Injury (2011)
  • Author(s): Imagama S, Sakamoto K, Tauchi R, Shinjo R, Ohgomori T, Ito Z, Zhang H, Nishida Y, Asami N, Takeshita S, Sugiura N, Watanabe H, Yamashita T, Ishiguro N, Matsuyama Y, Kadomatsu K
  • Journal Title: J Neurosci Volume: 31 Pages: 17091-17102
  • NAID: 120005946849
• [Journal Article] Cell surface heparin sulfate chains regulate local reception of FGF signaling in the mouse embryo (2011)
  • Author(s): 下川佳世
  • Journal Title: Developmental Cell Volume: 21 Issue: 2 Pages: 257-272
  • DOI: 10.1016/j.devcel.2011.06.027
  • Peer Reviewed
• [Journal Article] Baculovirus envelope protein ODV-66 is a novel chondroitinase with distinct substrate specificity. (2011)
  • Author(s): Sugiura N, Setoyama Y, Chiba M, Kimata K, Watanabe H
  • Journal Title: J Biol Chem Volume: 286 Issue: 33 Pages: 29026-29034
  • DOI: 10.1074/jbc.m111.251157
  • Peer Reviewed
• [Presentation] Role of large chondroitin sulfate proteoglycans in development and disease (2014)
  • Author(s): Hideto Watanabe
  • Organizer: 8th Annual Symposium in Institute of HUman-Environment Interface Biology
  • Place of Presentation: ソウル
  • Invited
• [Presentation] Roles of versican in cardiovascular development (2013)
  • Author(s): Sonoko Hatano, Shinji Makino, Koji Kimata, Hideto Watanabe
  • Organizer: International conference on hyaluronan
  • Place of Presentation: オクラホマ市
  • Invited
• [Presentation] Role of versican in heart and skeletal development (2013)
  • Author(s): Hideto Watanabe
  • Organizer: 9th Pan Pacific Connective Tissue Societies Symposium
  • Place of Presentation: 香港
  • Invited
• [Presentation] Chondroitin Sulfate synthase-2/chondroitin polymerizing factor : characterization and impact on chondroitin sulfate biosynthesis (2012)
  • Author(s): Hideto Watanabe, Hiroyasu Ogawa, Masafumi Shionyu, Nobui Sugiura, Sonoko Hatano, Naoko Nagai, Takashi Sato, Hisashi Narimatsu, Katsuji Shimizu, Koji Kimata
  • Organizer: 2012 Joint Meeting of the Society for Glycobiology & American Society for Matrix Biology
  • Place of Presentation: San Diego
• [Presentation] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth (2012)
  • Author(s): Ayuko Kono, Akiko Oguri, Kazuhisa Yokoo, Hideto Watanabe
  • Organizer: 23^<rd> FECTS and ISMB Joint Meeting
  • Place of Presentation: Katowice, Poland
• [Presentation] 間葉系組織の構築と維持におけるバーシカンの役割 (2012)
  • Author(s): 渡辺秀人
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] YAG laser treatment causes rapid degeneration and regeneration of collagen fibers in pig skin and facilitates fibroblast growth 23rd FECTS meeting (2012)
  • Author(s): Watanabe H
  • Organizer: 23rd FECTS meeting
  • Place of Presentation: カトヴィッツェ ポーランド
• [Presentation] Chondroitin sulfate synthase-2/chondroitin polymerizing factor: characterization and impact on chondroitin sulfate biosynthesis. (2012)
  • Author(s): Watanabe H
  • Organizer: Society for Glycobiology, American Society for Matrix Biology Joint Meeting 2012
  • Place of Presentation: サンディエゴ
• [Presentation] Chondroitin sulfate synthase-2/chondroitin polymerizing factor : characterization and impact on chondroitin sulfate biosynthesis (2011)
  • Author(s): Hiroyasu Ogawa, Masashi Shionyu, Nobuo Sugiura, Sonoko Hatano, Naoko Nagai, Takashi Sato, Masanori Gotoh, Hisashi Narimatsu, Katsuji Shimizu, Koji Kimata, Hideto Watanabe
  • Organizer: 7th International Conference on Proteoglycans
  • Place of Presentation: Sydney
• [Presentation] 軟骨と関節の形成・維持におけるバーシカンの役割
  • Author(s): 渡辺 秀人
  • Organizer: 日本骨代謝学会(招待講演)
  • Place of Presentation: 大阪
• [Remarks] 愛知医科大学分子医科学研究所
  • URL: http://www.aichi-med-u.ac.jp/su10/su1009/index.html
• [Remarks] 愛知医科大学 分子医科学研究所
  • URL: http://www.aichi-med-u.ac.jp/su10/su1009/index.html
• [Remarks] 愛知医科大学 戦略的研究基盤形成支援事業
  • URL: http://amu-strategy.org/