Early andspecific detection of unstable atherosclerotic pl:aqTuiesssue factor imaging using pre-targeting systems

• Project/Area Number: 23659573
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Hokkaido University
• Principal Investigator: KUGE Yuji 北海道大学, アイソトープ総合センター, 教授 (70321958)
• Co-Investigator(Kenkyū-buntansha): ZHAO Songji 北海道大学, 大学院・医学研究科, 教授 (80374239) ; NISHIJIMA Ken-ichi 北海道大学, 大学院・医学研究科, 特任講師 (60364254) ; 川井 恵一 金沢大学, 保健学系, 教授 (30204663) ; 山下 篤 宮崎大学, 医学部, 助教 (90372797)
• Co-Investigator(Renkei-kenkyūsha): KAWAI Keiichi 金沢大学, 保健学系, 教授 (30204663) ; YAMASHITA Atsushi 宮崎大学, 医学部, 助教 (90372797) ; NISHIJIMA Ken-ichi 北海道大学, 大学院・医学研究科, 特任講師 (60364254) ; SHIMIZU Youichi 北海道大学, アイソトープ総合センター, 助教 (90634212)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 放射線 / 核医学診断 / 動脈硬化 / 不安定プラーク / 組織因子

Research Abstract

We performed several experiments to establish a new nuclear medicine technique for imaging unstable atherosclerotic plaques through specific detection of tissue factor (TF). Consequently, we obtained several new findings concerning: 1) synthesis methods of 18F-laveled biotin-derivative, 2) PET imaging protocols and TF expression levels in our rabbit model, 3) accumulation of anti-rabbit TF monoclonal antibody in atherosclerotic lesions, and 4) blood clearance of anti-rabbit TF monoclonal antibody. These findings provided important information to establish a new nuclear medicine technique for TF imaging.

Research Products

• [Journal Article] A rabbit model of thrombosis on atherosclerotic lesions. (2011)
  • Author(s): Yamashita A, Asada Y.
  • Journal Title: J Biomed Biotechnol. Volume: 2011 Issue: 1 Pages: 1-15
  • DOI: 10.1155/2011/424929
  • Peer Reviewed
• [Journal Article] Arterial 18F-fluorodeoxyglucose uptake reflects thrombus formation and tissue factor expression via nuclear factor-κB in rabbit atherosclerotic lesions
  • Author(s): Atsushi Yamashita, Yan Zhao, Songji Zhao, Yunosuke Matsuura, Chihiro Sugita, Takashi Iwakiri, Nozomi Okuyama, Kazuyo Ohe, Chihiro Koshimoto, Keiichi Kawai, Nagara Tamaki, Yuji Kuge, and Yujiro Asada
  • Journal Title: Circulation Journal Volume: (In press)
• [Presentation] 18F-fluorodeoxyglucose uptake reflects thrombus formation and tissue factor expression via nuclear factor-kB in rabbit atherosclerotic lesions (2012)
  • Author(s): Atsushi Yamashita, Yan Zhao, Yunosuke Matsuura, Kazuaki Yamasaki Chihiro Sugita, Keiichi Kawai, Nagara Tamaki, Songji Zhao, Yuji Kuge, Yujiro Asada
  • Organizer: American Heart Association Scientific Sessions 2012
  • Place of Presentation: Los Angeles Convention Center, Los Angeles, CA, U.S.A
• [Presentation] 動脈血栓能を反映する18F-フルオロデオキシグルコースの血管集積 (2012)
  • Author(s): 山下篤、趙芫、趙松吉、松浦祐之介、川井恵一、玉木長良、久下裕司、浅田祐士郎
  • Organizer: 第52回日本核医学会学術総会。
  • Place of Presentation: ロイトン札幌
• [Presentation] Thrombus formation on disrupted plaques (2011)
  • Author(s): Yamashita A
  • Organizer: XXIII congress of International Society on Thrombosis and Haemostasis(招待講演)
  • Place of Presentation: Kyoto International Conference Center (Kyoto)
• [Presentation] 動脈血栓能を反映する18F-フルオロデオキシグルコースの血管集積
  • Author(s): 山下 篤
  • Organizer: 第52回日本核医学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Remarks]
  • URL: http://www.hokudai.ac.jp/radiois/kenkyu/kenkyu_kenkyusitsu_gaiyou.html