In vivo tracking of transplanted cells using manganese-enhanced magnetic resonance imaging(MEMRI) and assessment of cardiac function for mouse myocardial infarction

• Project/Area Number: 23659600
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: ODAKA Kenichi 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, 研究員 (20443062)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 磁気共鳴画像 / 細胞移植 / マンガン造影 / 心機能 / 虚血性疾患 / 血管新生 / 末梢血単核球 / 心臓超音波

Research Abstract

In-vivo tracking of transplanted Mn^<2+>-labeled cells with Magnetic Resonance Imaging(MRI) was attempted to visualize the localization of migrated cells at high spatial resolution in mouse myocardial infarction and the long term regenerative therapeutic effects. MRI measured ejection fraction(EF) was lower than EF measured by ultrasound. However, EF of MRI showed a good correlation to that of ultrasound. Repeat measurement showed slight change of EF in both MRI and ultrasound. Transplanted Mn^<2+>-labeled cells were visualized by MRI up to 4 weeks after transplantation. Cell tracking with manganese-enhanced MRI and assessment of cardiac function promise to be a standard technique for the cell selection and delivery.

Research Products

• [Journal Article] In vivo tracking of transplanted mononuclear cells using manganese-enhanced magnetic resonance imaging (2011)
  • Author(s): Odaka K, Minamino T, et al
  • Journal Title: PLos One Volume: 6 Issue: 10 Pages: e25487-e25487
  • DOI: 10.1371/journal.pone.0025487
  • Peer Reviewed
• [Journal Article] Toward in vivo imaging of heart disease using a radiolabeled single-chain Fv fragment targeting tenascin-C (2011)
  • Author(s): Norihiro Kobayashi, Kenichi Odaka, Tomoya Uehara, Kyoko Imanaka-Yoshida, Yoshonori Kato, Hiroyuki Oyama, Hiroyuki Tadokoro, Hiromichi Akizawa, Shuji Tanada, Michiaki Hiroe, Toshimitsu Fukumura, Issei Komuro, Yasushi Arano, Toshimichi Yoshida, Toshiaki Irie
  • Journal Title: Journal of Analytical Chemistry Volume: 83巻 Pages: 9123-9130
  • URL: http://dx.doi.org/10.1021/ac202159p
  • Peer Reviewed
• [Journal Article] Toward in vivo imaging of heart disease using a radiolabeled single-chain Fv fragment targeting tenascin-C (2011)
  • Author(s): Kobayashi N, Odaka K, Uehara T, Imanaka-Yoshida K, Kato Y, Oyama H, Tadokoro H, Akizawa H, Tanada S, Hiroe M, Fukumura T, Komuro I, Arano Y, Yoshida T, Irie T
  • Journal Title: ANALYTICAL CHEMISTRY Volume: 83 Issue: 23 Pages: 9123-9130
  • DOI: 10.1021/ac202159p
  • Peer Reviewed
• [Presentation] 急性心筋梗塞マウスのマンガン標識移植細胞追跡とシネMRIによる心機能評価 (2011)
  • Author(s): 小高謙一、青木伊知男、黒岩大悟、Jeff Kershaw、菊池達矢、小畠隆行、佐賀恒夫、福村利光、森谷純治、舘野馨、岡田将、南野徹、小林欣夫、田所裕之、小室一成
  • Organizer: 第34回千葉大学循環病態医科学・循環器内科懇話会
  • Place of Presentation: 京成ホテルミラマーレ(千葉県)
  • Year and Date: 2011-12-11
• [Presentation] マンガン標識末梢血単核球のラット脳への移植における動態追跡 (2011)
  • Author(s): 小高謙一、伊藤康一、青木伊知男、島田拓也、森谷純治、舘野馨、南野徹、小室一成、下山一郎、佐賀恒夫、福村利光
  • Organizer: 第39回日本磁気共鳴医学会大会
  • Place of Presentation: リーガロイヤルホテル小倉(福岡県)
  • Year and Date: 2011-09-29
• [Presentation] マンガン標識末梢血単核球のラット脳への移植における動態追跡 (2011)
  • Author(s): 小高謙一
  • Organizer: 第39回日本磁気共鳴医学会大会
  • Place of Presentation: リーガロイヤルホテル小倉,(福岡県)
  • Year and Date: 2011-09-29
• [Presentation] 心筋梗塞ラットのマンガン標識移植細胞追跡とシネMRIによる機能評価 (2011)
  • Author(s): 小高謙一、青木伊知男、森谷純治、中原鉄平、田所裕之、舘野馨、菊池達矢、南野徹、福村利光、佐賀恒夫、小室一成
  • Organizer: 第6回日本分子イメージング学会総会・学術集会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2011-05-26
• [Presentation] In-vivo tracking of transplanted stem cells labeled with manganese using magnetic resonance imaging. Workshop for iPS cell study promotion (2011)
  • Author(s): Kenichi Odaka
  • Organizer: Japan Science and Technology Agency-California Institute for Regenerative Medicine 2011
  • Place of Presentation: 神戸ポートピアホテル(兵庫県)
  • Year and Date: 2011-05-17
• [Presentation] In-vivo tracking of transplanted stem cells labeled with manganese using magnetic resonance imaging (2011)
  • Author(s): 小高謙一
  • Organizer: Workshop for iPS cell study promotion, Japan Science and Technology Agency-California Institute for Regenerative Medicine 2011
  • Place of Presentation: 神戸ポートピアホテル(兵庫県)
  • Year and Date: 2011-05-17