Study of hydroxymethlated DNA and TET gene family in cancer
Project/Area Number |
23659610
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General surgery
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Research Institution | Kyoto University |
Principal Investigator |
SATO FUMIAKI 京都大学, 医学(系)研究科(研究院), 准教授 (20467426)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 乳癌 / ハイドロキシメチル化 / ハイドロオキシメチル化 / メチル化 / エピジェネティクス / がん / TET |
Research Abstract |
The aim of this study is to clarify the significance of hydroxymethyl CpG (hmCpG) in breast cancer cells. The hmCpG is thought to be the first step in demethylation mechanism of methylated CpG, and to play an important roles in epigenetic gene regulation in cancer. In this study, to screen differentially hydroxymethylated regions (DHMR), we conducted to methylation microarray analysis to identify differentially hypomethylated regions, using ATCC's breast cancer cell line panels. According to the detailed analysis, we identified subtype specific hypomethylated regions in Luminal type and triple negative breast cancers, which would be candidate regions of DHMR. On the other hand, we established a gene induction system of TET1/2/3 gene families, using Lenti-virus vector system. It will help the further functional analysis of TET1/2/3 genes in breast cancer cells.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Genetic heterogeneity of hepatitis C virus in association with antiviral therapy determined by ultra-deep sequencing2011
Author(s)
Nasu A, Marusawa H, Ueda Y, Nishijima N, Takahashi K, Osaki Y, Yamashita Y, Inokuma T, Tamada T, Fujiwara T, Sato F, Shimizu K, Chiba T
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Journal Title
PLoS One
Volume: 6
Issue: 9
Pages: e24907-e24907
DOI
NAID
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Peer Reviewed
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[Presentation] Development of microRNA-based prediction model of Trastuzumab treatment response for HER2-positive breast cancer using FFPE specimens2013
Author(s)
Fumiaki Sato, ZhipengWang, Takayuki Ueno, Akira Myotomo, Satoko Takizawa, Feng Ling Pu, Norikazu Masuda, Yoshiki Mikami, Kazuharu Shimizu, Shigehira Saji, Masakazu Toi
Organizer
AACR 2013
Place of Presentation
Washington DC
Related Report
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[Presentation] Nuclear location of S6K is associated with unfavorable disease-free survival in hormone-receptor positive breast cancer cases.2013
Author(s)
Nobuko Kawaguchi-Sakita, Rafaat AEB Mohamed, Fumiaki Sato, Takayuki Ueno, Masahiro Kawashima, Noriyuki Yoshida, Wen Zhao Li, Tomoharu Sugie, Yoshiki Mikami, Shigehira Saji, Takashi Inamoto, Masakazu Toi
Organizer
AACR 2013
Place of Presentation
Washington DC
Related Report
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