| Project/Area Number |
23659612
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MIYAGAWA Shuji 大阪大学, 大学院・医学系研究科, 准教授 (90273648)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 異種移植 / トランスジェニックブタ / ハイブリッド遺伝子 / 顕微授精 / 遺伝子構築 / nsulin promoter / C1-INH / Thrombomodulin / DAF / MCP / 胚移植試験 / 移植・再生医療 / 応用動物 / トランスレーションリサーチ / 動物 / バイオテクノロジー |
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| Research Abstract |
T o establish transgenic pigs with multiple human genes, the hybrid gene containing the function domains of each molecule were produced and the pigs with the gene was then tried to produce by intracytoplasmic sperm injection(ICSI) methods. At first, the transgenic mouse with the hybrid genes were successfully produced and analyzed. Concerning the pig project, the condition of the oocyte with each gene was studied under the microscope. The condition of the segmentation was no problem, however the pigs with genes were not born in this period.
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