Novel Innovative Safety Reprograming Technology by MicroRNAs

• Project/Area Number: 23659617
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Osaka University
• Principal Investigator: ISHII Hideshi 大阪大学, 医学系研究科, 寄附講座教授 (10280736)
• Co-Investigator(Kenkyū-buntansha): MORI Masaki 大阪大学, 大学院・医学系研究科, 教授 (70190999)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / 外科学 / ES 細胞 / ES細胞

Research Abstract

For innovative cancer therapy, we studied the novel methods to induce microRNA-based induced pluripotent stem cells (miPS) by introduction of synthetic oligo microRNA. This novel technology would be able to apply the broad range of gastrointestinal diseases such as human cancer.

Research Products

• [Journal Article] SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers. (2013)
  • Author(s): Suzuki, Y., Haraguchi, N., Takahashi, H., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Mizushima, T., Ishii, H., Doki, Y., Mori, M., Yamamoto, H
  • Journal Title: Int. J. Oncol. Volume: 42(1) Pages: 161-167
• [Journal Article] Depletion of Jarid1b induces cellular senescence in human colorectal cancer. (2013)
  • Author(s): Ohta, K., Haraguchi, N., Kano, Y., Kagawa, Y., Konno, M., Nishikawa, S., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Nishimura, T., Noguchi, Y., Ozaki, M., Kudo, T., Sakai, D., Satoh, T., Fukami, M
  • Journal Title: Intern. J. Oncol. Volume: 42(4) Pages: 1212-1218
• [Journal Article] SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers. (2013)
  • Author(s): Suzuki, Y., Haraguchi, N., Takahashi, H., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Mizushima, T., Ishii, H., Doki, Y., Mori, M., Yamamoto, H.
  • Journal Title: Int. J. Oncol. Volume: 42 Pages: 161-167
  • DOI: 10.3892/ijo.2012.1713
  • Peer Reviewed
• [Journal Article] Depletion of Jarid1b induces cellular senescence in human colorectal cancer. (2013)
  • Author(s): Ohta, K., Haraguchi, N., Kano, Y., Kagawa, Y., Konno, M., Nishikawa, S., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Uemura, M., Nishimura, J., Hata, T., Takemasa, I., Nishimura, T., Noguchi, Y., Ozaki, M., Kudo, T., Sakai, D., Satoh, T., Fukami, M., Ishii, M., Yamamoto, H., Doki, Y., Mori, M., Ishii, H.
  • Journal Title: Intern. J. Oncol. Volume: 42 Issue: 4 Pages: 1212-1218
  • DOI: 10.3892/ijo.2013.1799
  • Peer Reviewed
• [Journal Article] Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance. (2012)
  • Author(s): Hashiguchi, Y., Nishida, N., Mimori, K., Sudo, T., Tanaka, F., Shibata, K., Ishii, H., Mochizuki, H., Hase, K., Doki, Y., Mori, M.
  • Journal Title: Int. J. Oncol Volume: 40(5) Pages: 1477-1482
• [Journal Article] Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma. (2012)
  • Author(s): Yokobori, T., Mimori, K., Iwatsuki, M., Ishii, H., Tanaka, F., Sato, T., Toh, H., Sudo, T., Iwaya, T., Tanaka, Y., Onoyama, I., Kuwano, H., Nakayama, K.I., Mori, M.
  • Journal Title: Int. J. Oncol Volume: 41(1) Pages: 253-259
• [Journal Article] Nagano, H., Doki, Y., Mori, M. Genotoxic therapy stimulates error-prone DNA repair in dormant hepatocellular cancer stem cells. (2012)
  • Author(s): Nishikawa, S., Ishii, H., Haraguchi, N., Kano, Y., Fukusumi, T., Ohta, K., Ozaki, M., Sakai, D., Satoh, T.,Nagano, H., Doki, Y., Mori, M.
  • Journal Title: Exp. Ther. Med. Volume: 3(6) Pages: 959-962
• [Journal Article] Absence of CD71 transferrin receptor characterizes human gastric adenosquamous carcinoma stem cells (2012)
  • Author(s): Ohkuma, M., Haraguchi, N., Ishii, H., Mimori, K., Tanaka, F., Kim, H.M., Shimomura, M., Hirose, H., Yanaga, K., Mori, M.
  • Journal Title: Ann. Surg. Oncol. Volume: 19(4) Pages: 1357-1364
• [Journal Article] Increased CD13 expression reduces reactive oxygen species, promoting survival of liver cancer stem cells via an epithelial-mesenchymal transition-like phenomenon. (2012)
  • Author(s): Kim, H.M., Haraguchi, N., Ishii, H., Ohkuma, M., Okano, M., Mimori, K., Eguchi, H., Yamamoto, H., Nagano, H., Sekimoto, M., Doki, Y., Mori, M.
  • Journal Title: Ann. Surg. Oncol. Volume: 19
• [Journal Article] Microarray analysis of colorectal cancer stromal tissue reveals upregulation of two oncogenic microRNA clusters. (2012)
  • Author(s): Nishida, N., Nagahara, M., Sato, T., Mimori, K., Sudo, T., Tanaka, F., Shibata, K., Ishii, H., Sugihara, K., Doki, Y., Mori, M.
  • Journal Title: Clin. Cancer Res. Volume: 18(11) Pages: 3054-3070
• [Journal Article] Transcriptome study of dormant gastrointestinal cancer stem cells. (2012)
  • Author(s): Nishikawa, S., Dewi, D.L., Ishii, H., Konno, M., Haraguchi, N., Kano, Y., Fukusumi, T., Ohta, K., Noguchi, Y., Ozaki, M., Sakai, D., Satoh, T., Doki, Y., Mori, M.
  • Journal Title: Int. J. Oncol. Volume: 41(3) Pages: 979-984
• [Journal Article] Dicer 1, ribonuclease type III modulates a reprogramming effect in colorectal cancer cells. (2012)
  • Author(s): Dewi, D.L., Ishii, H., Haraguchi, N., Nishikawa, S., Kano, Y., Fukusumi, T., Ozaki, M., Saito, T., Sakai, D., Satoh, T., Doki, Y., Mori, M.
  • Journal Title: Int J Mol Med. Volume: 29(6) Pages: 1060-1064
• [Journal Article] Hypoxia and TP53 deficiency for induced pluripotent stem cell-like properties in gastrointestinal cancer. (2012)
  • Author(s): Hoshino, H., Nagano, H., Haraguchi, N., Nishikawa, S., Tomokuni, A., Kano, Y., Fukusumi, T., Saito, T., Osaki, M., Sakai, D., Satoh, T., Eguchi, H., Sekimoto, M., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Intern. J. Oncol. Volume: 40(5) Pages: 1423-1230
• [Journal Article] Down-regulation of miR-125a-3p in human gastric cancer and its clinicopathological significance (2012)
  • Author(s): Yojiro Hashiguchi, Naohiro Nishida, Koshi Mimori, Tomoya Sudo, Fumiaki Tanaka, Kohei Shibata, Hideshi Ishii, Hidetaka Mochizuki, Kazuo Hase, Yuichiro Doki, Masaki Mori
  • Journal Title: Int J Oncol Volume: 40(5) Pages: 1477-82
  • DOI: 10.3892/ijo.2012.1363
  • Peer Reviewed
• [Journal Article] Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma (2012)
  • Author(s): Mar ; 113(2): 204-9. 2. Yokobori T, Mimori K, Iwatsuki M, Ishii H, Tanaka F, Sato T, Toh H, Sudo T, Iwaya T, Tanaka Y, Onoyama I, Kuwano H, Nakayama KI, Mori M.
  • Journal Title: Int J Oncol Volume: 41(1) Pages: 253-9
  • DOI: 10.3892/ijo.2012.1436
  • Peer Reviewed
• [Journal Article] Genotoxic therapy stimulates error-prone DNA repair in dormant hepatocellular cancer stem cells. (2012)
  • Author(s): Nishikawa, S.,et.al
  • Journal Title: Exp. Ther. Med Volume: 3 Pages: 959-962
  • Peer Reviewed
• [Journal Article] Absence of CD71 transferrin receptor characterizes human gastric adenosquamous carcinoma stem cells (2012)
  • Author(s): Ohkuma M, Haraguchi N, Ishii H, Mimori K, Tanaka F, Kim HM, Shimomura M, Hirose H, Yanaga K, Mori M.
  • Journal Title: Ann Surg Oncol Volume: 19(4) Issue: 4 Pages: 1357-64
  • DOI: 10.1245/s10434-011-1739-7
  • Peer Reviewed
• [Journal Article] Microarray Analysis of Colorectal Cancer Stromal Tissue Reveals Upregulation of Two Oncogenic miRNA Clusters (2012)
  • Author(s): Nishida N, Nagahara M, Sato T, Mimori K, Sudo T, Tanaka F, et al
  • Journal Title: Clinical Cancer Reserch Volume: (Epub) Issue: 11 Pages: 3054-3070
  • DOI: 10.1158/1078-0432.ccr-11-1078
  • Peer Reviewed
• [Journal Article] Transcriptome study of dormant gastrointestinal cancer stem cells. (2012)
  • Author(s): Nishikawa, S.,et.al
  • Journal Title: Int. J. Oncol Volume: 41 Issue: 3 Pages: 979-984
  • DOI: 10.3892/ijo.2012.1531
  • Peer Reviewed
• [Journal Article] Dicer 1, ribonuclease type III modulates a reprogramming effect in colorectal cancer cells (2012)
  • Author(s): Dewi DL, Ishii H, Haraguchi N, Nishikawa S, Kano Y, Fukusumi T, Ozaki M, Saito T, Sakai D, Satoh T, Doki Y, Mori M.
  • Journal Title: Int J Mol Med Volume: 29(6) Pages: 1060-4
  • DOI: 10.3892/ijmm.2012.945
  • Peer Reviewed
• [Journal Article] Hypoxia and TP53 deficiency for induced pluripotent stem cell-like properties in gastrointestinal cancer (2012)
  • Author(s): Hoshino H, Nagano H, Haraguchi N, Nishikawa S, Tomokuni A, Kano Y, Fukusumi T, Saito T, Ozaki M, Sakai D, Satoh T, Eguchi H, Sekimoto M, Doki Y, Mori M, Ishii H.
  • Journal Title: Int J Oncol Volume: 40(5) Pages: 1423-30
  • DOI: 10.3892/ijo.2012.1346
  • Peer Reviewed
• [Journal Article] Increased CD13 Expression Reduces Reactive Oxygen Species, Promoting Survival of Liver Cancer Stem Cells via an Epithelial-Mesenchymal Transition-like Phenomenon (2011)
  • Author(s): Kim HM, Haraguchi N, Ishii H, Ohkuma M, Okano M, Mimori K, Eguchi H, Yamamoto H, Nagano H, Sekimoto M, Doki Y, Mori M.
  • Journal Title: Ann Surg Oncol Volume: 31 Issue: S3 Pages: 539-548
  • DOI: 10.1245/s10434-011-2040-5
  • Peer Reviewed
• [Journal Article] Gain-of-function oncogenic mutations in TP53 enhance defined factor-mediated cellular reprogramming (2011)
  • Author(s): Moon JH., Ishii H., Dewi DL., Kano Y., Yamamoto K., Nishikawa S., Fujiwara Y., Doki Y., Mori M.
  • Journal Title: Nat Preceding Volume: －
  • DOI: 10.1038/npre.2011.5681.1
  • Peer Reviewed
• [Journal Article] The future prospects and subject of the liver cancer stem cells study for the clinical application. (2011)
  • Author(s): Haraguchi N, et.al
  • Journal Title: Gastroenterology Volume: Epub
  • Peer Reviewed
• [Journal Article] Combination use of anti-CD133 antibody and SSA lectin can effectively enrich cells with high tumorigenicity (2011)
  • Author(s): MoriwakiK、Okudo K, Miyoshi E, et al
  • Journal Title: Cancer Science Volume: 102 Issue: 6 Pages: 1164-1170
  • DOI: 10.1111/j.1349-7006.2011.01923.x
  • NAID: 10029294388
  • Peer Reviewed
• [Journal Article] Significance of Lgr5(+ ve) cancer stem (2011)
  • Author(s): Takahashi H, Ishii H, Nishida N, Takemasa I, Mizushima T, Ikeda M, Yokobori T, Mimori K, Yamamoto H, Sekimoto M, Doki Y, Mori M.
  • Journal Title: cells in the colon and rectum Ann Surg Oncol Volume: 18(4) Issue: 4 Pages: 1166-74
  • DOI: 10.1245/s10434-010-1373-9
  • Peer Reviewed
• [Journal Article] The Significance of PTTX2 Overexpression in Human Colorectal Cancer (2011)
  • Author(s): Hirose Hajime, Ishii Hideshi, Mimori Koshi, et al
  • Journal Title: ANNALS OF SURGICAL ONCOLOGY Volume: 18 Issue: 10 Pages: 3005-3012
  • DOI: 10.1245/s10434-011-1653-z
  • Peer Reviewed
• [Journal Article] MicroRNA-125a-5p is an independent prognostic factor in gastric cancer, and inhibits the proliferation of human gastric cancer cells in combination with trastuzumab (2011)
  • Author(s): Nishida N, Mimori K, Fabbri M, Yokobori T, Sudo T, Tanaka F, Shibata K, Ishii H, Doki Y, Mori M.]
  • Journal Title: Clin Cancer Res Volume: 17(9) Issue: 9 Pages: 2725-33
  • DOI: 10.1158/1078-0432.ccr-10-2132
  • Peer Reviewed
• [Journal Article] Identification of recurrence-related microRNAs in the bone marrow of breast cancer patients. (2010)
  • Author(s): Ota D, Mimori K, Yokobori T, Iwatsuki M, Kataoka A, Masuda N, Ishii H, Ohno S, Mori M.
  • Journal Title: Int J Oncol 38 Volume: 38(4) Issue: 4 Pages: 955-962
  • DOI: 10.3892/ijo.2011.926
  • Peer Reviewed
• [Presentation] Leucine-rich repeat-containing G-protein-coupled receptor5(Lgr5)のヒト腸管における発現解析 (2012)
  • Author(s): 浜部敦史 他13名
  • Organizer: 第23回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-15
• [Presentation] 胃癌幹細胞の同定とその生物学的解析 (2012)
  • Author(s): 西川晋平 他14名
  • Organizer: 第23回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-15
• [Presentation] Cancer stem cell research for innovative cell-modifying technologies (2012)
  • Author(s): 石井秀始
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-19
• [Presentation] 先端医療:がん幹細胞理論に基づく新規治療開発 (2012)
  • Author(s): 石井秀始
  • Organizer: 第10回日本臨床腫瘍学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-19
• [Presentation] Induced Pluripotent Stem Cell Reprogramming for Innovative Cell-Modifying Technology in Cancer (2012)
  • Author(s): 石井秀始
  • Organizer: 第33回日本炎症・再生医学学会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2012-07-05
• [Presentation] Leucine-rich repeat-containing G-protein-coupled receptor５（Lgr５）のヒト腸管における発現解析 (2012)
  • Author(s): 浜部敦史 他13名
  • Organizer: 第２３回日本消化器癌発生学会総会
  • Place of Presentation: ルネッサンスリゾートナルト（徳島県）
• [Presentation] 胃癌幹細胞の同定とその生物学的解析 (2012)
  • Author(s): 西川晋平 他14名
  • Organizer: 第２３回日本消化器癌発生学会総会
  • Place of Presentation: ルネッサンスリゾートナルト（徳島県）
• [Presentation] Cancer stem cell research for innovative cell-modifying technologies (2012)
  • Author(s): 石井秀始
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] 先端医療：がん幹細胞理論に基づく新規治療開発 (2012)
  • Author(s): 石井秀始
  • Organizer: 第10回日本臨床腫瘍学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪府）
• [Presentation] Induced Pluripotent Stem Cell Reprogramming for Innovative Cell-Modifying Technology in Cancer (2012)
  • Author(s): 石井秀始
  • Organizer: 第33回日本炎症・再生医学会シンポジウム
  • Place of Presentation: ホテル日航福岡（福岡県）
• [Presentation] 肝臓癌幹細胞の分離・同定と治療標的化へ向けた検討 (2011)
  • Author(s): 原口 直紹, 石井 秀始, 永野 浩昭, 土岐 祐一郎, 森 正樹
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都市国際交流会館(京都府)
• [Presentation] 癌・固形腫瘍の診断・治療 (2011)
  • Author(s): 石井秀始、大重英行
  • Organizer: 第21回日本サイトメトリ‐学会学術集会
  • Place of Presentation: 京都市国際交流会館(京都府)
• [Presentation] 肝細胞癌の再発・転移を制御するmicroRNAの探索 (2011)
  • Author(s): 友國 晃 他10名
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都市国際交流会館(京都府)