| Project/Area Number |
23659620
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
KOIKE Naoto 横浜市立大学, 医学研究科, 客員研究員 (50301081)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Yasuharu 横浜市立大学, 医学部, 助教 (60375235)
武部 貴則 横浜市立大学, 医学部, 助手 (20612625)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKEBE Takanori 横浜市立大学, 医学部, 助手 (20612625)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 外科総論 / 肝組織再構築 / 微小血管網 / イメージング |
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| Research Abstract |
In this study, we aimed to establish a visualization system to monitor liver organogenesis from liver bud in vivo. To this end, we developed a liver bud generation method according to the three-dimensional co-culture of human liver stem cells, human umbilical vein endothelial cells (HUVEC) and human mesenchymal stem cells (hMSC). Next, we established a 4D imaging system using ultra-high-speed confocal microscope system. Then we transplanted the human liver bud into the cranial window and observed visualized and reconstituted human liver tissue with human vascular network. We have established an imaging technology to visualize and analyze the formation of human solid organs.
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