Formation of insulin-producing cells from iPS cells in conjunction with islet transplantation

• Project/Area Number: 23659627
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Fukuoka University
• Principal Investigator: YASUNAMI Yohichi 福岡大学, 医学部, 教授 (00166521)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 移植外科学 / インスリン産生細胞 / iPS細胞 / インスリン / 膵島移植 / 糖尿病 / 再生医療

Research Abstract

In this study, we attempted to generate insulin-producing cells from mouse iPS cells by co-transplantation with isolated islets (rat, porcine) beneath the kidney capsule of STZ-diabetic nude mice since islets may serve to provide an appropriate circumstances as “nitch”. As a result, clusters of insulin producing cells were found in tumors at 30 days after transplantation. Currently, the study with the use of iPS cells transfected with transcription factors including Pdx1, Ngn3 and MafA) is under investigation.

Research Products

• [Journal Article] Pretreatment of donor islets with the Na+/Ca2+ exchanger inhibitor improves the efficiency of islet transplantation. (2013)
  • Author(s): Mera T, Itoh T, Kita S, Kodama S, Kojima D, Nishinakamura H, Okamoto K, Ohkura M, Nakai J, Iyoda T, Iwamoto T, Matsuda T, Baba A, Omori K, Ono J, Watarai H, Taniguchi M, Yasunami Y.
  • Journal Title: Am J Transplant Volume: in press
  • Peer Reviewed
• [Journal Article] Prevention of High-Mobility Group Box 1-Mediated Early Loss of Transplanted Mouse Islets in the Liver by Antithrombin III. (2012)
  • Author(s): Kojima D, Mera T, Nishinakamura H, Itoh T, Ogata T, Matsuoka N, Kodama S, Yasunami Y.
  • Journal Title: Transplantation Volume: 93(10) Issue: 10 Pages: 983-8
  • DOI: 10.1097/tp.0b013e31824d3508
  • Peer Reviewed
• [Journal Article] Prevention of High-Mobility Group Box 1-Mediated Early Loss of Transplanted Mouse Islets in the Liver by Antithrombin III (2012)
  • Author(s): Kojima D, Mera T, Nishinakamura H, Itoh T, Ogata T, Matsuoka N, Kodama S, Yasunami Y.
  • Journal Title: Transplantation Volume: In press
  • Peer Reviewed
• [Journal Article] 糖尿病の新しい治療としての膵島細胞移植 (2011)
  • Author(s): 安波洋一
  • Journal Title: 九州実験動物雑誌 Volume: 27 Pages: 23-26
  • Peer Reviewed
• [Journal Article] 膵島移植とHMGB1 (2011)
  • Author(s): 安波洋一
  • Journal Title: バイオメヂカル Volume: 21 Pages: 42-46
  • Peer Reviewed
• [Presentation] Inhibitory effect of thrombomodulin on HMGB1-stimulated IFN-γ production of hepatic NKT and Gr-1+ Cells, facilitating to prevent early loss of transplanted islets in the liver of mice. (2012)
  • Author(s): Kojima D, Nishinakamura H, Ogata T, Nagaishi M, Mera T, Itoh T, Matsuoka N, Kodama S, Yasunami Y.
  • Organizer: 24th International Congress of the Transplantation society
  • Place of Presentation: Berlin, Germany
• [Presentation] Innate immunity in Transplantation (2011)
  • Author(s): 安波洋一
  • Organizer: The 2011 Fall Conference of the Korean Association of Immunologists(招待講演)
  • Place of Presentation: Seoul
• [Presentation] Targeting HMGB1 in Transplantation (2011)
  • Author(s): 安波洋一
  • Organizer: 3rd HMGB1 International Worshop(招待講演)
  • Place of Presentation: Pittsburgh, USA
• [Remarks] グローバルFUプログラムホームページ
  • URL: http://www.med.fukuoka-u.ac.jp/globalfu/