Photodynamic diagnosis and treatment for local multiple-drug-resistant bacterial infection using photosensitizers that preferably accumulate into bacteria.
| Project/Area Number |
23659628
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | National Defense Medical College |
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Principal Investigator |
MORIMOTO YUJI 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科専門課程, 准教授 (10449069)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Manabu 防衛医科大学校, 医学教育部医学科専門課程, 准教授 (70531391)
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| Research Collaborator |
HAMBLIN Michael R Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 光線力学療法 / 薬剤耐性菌 / 発光細菌 / MRSA / メチレンブルー / 膝関節炎 / 多剤耐性菌 / マウス |
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| Research Abstract |
With an established murine intractable MRSA arthritis model using bioluminescent MRSA and resin microparticle, we examined both therapeutic and preventive effect of PDT using methylene blue. Although bioluminescent intensity of MRSA was not diminished immediately after PDT, it was gradually decreased for 6 days after PDT. The therapeutic effect was lost by intravenous administration of anti-neutrophil antibody. Moreover, pre-conditioning PDT on the normal knee joint prevented an exacerbation of MRSA infection of the knee joint. The preventive effect was also lost by intravenous administration of anti-neutrophil antibody. On the other hand, an adjuvant use of antibiotics for MRSA either linezolid or vancomycin exerted no enhancing effect of MB-PDT, suggesting that they might have inhibited the PDT induced accumulating effect of neutrophils into infected lesions.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Linezolid and vancomycin decrease the therapeutic effect of methylene blue-photodynamic therapy in a mouse model of MRSA bacterial arthritis2013
Author(s)
Tanaka M, Mroz P, Dai T, Huang L, Morimoto Y, Kinoshita M, Yoshihara Y, Shinomiya N, Seki S, Nemoto K, Hamblin MR
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Journal Title
Photochem Photobiol
Volume: 89(3)
Pages: 679-682
Related Report
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[Journal Article] Linezolid and vancomycin decrease the therapeutic effect of methylene blue-photodynamic therapy in a mouse model of MRSA bacterial arthritis2013
Author(s)
Tanaka, M. Mroz, P. Dai, T. Huang, L. Morimoto, Y. Kinoshita, M. Yoshihara, Y. Shinomiya, N. Seki, S. Nemoto, K. Hamblin, M. R.
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Journal Title
Photochem Photobiol
Volume: 89(3)
Issue: 3
Pages: 679-82
DOI
Related Report
Peer Reviewed
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[Journal Article] Photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation2012
Author(s)
Tanaka M, Mroz P, Dai T, Huang L, Morimoto Y, Kinoshita M, Yoshihara Y, Nemoto K, Shinomiya N, Seki S, Hamblin MR
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Journal Title
Related Report
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[Journal Article] Optimal Photosensitizers for Photodynamic Therapy of Infections Should Kill Bacteria but Spare Neutrophils2012
Author(s)
Tanaka M, Kinoshita M, Yoshihara Y, Shinomiya N, Seki S, Nemoto K, Hirayama T, Dai T, Huang L, Hamblin MR, Morimoto Y
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Journal Title
Photochem Photobiol
Volume: 88(1)
Pages: 227-232
Related Report
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[Journal Article] Photodynamic therapy using intra-articular Photofrin for murine MRSA arthritis : biphasic light dose response for neutrophil-mediated antibacterial effect2011
Author(s)
Tanaka, M., Kinoshita, M., Yoshihara, Y., Shinomiya, N., Seki, S., Nemoto, K., Hamblin, M.R., Morimoto, Y.
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Journal Title
Lasers Surg Med
Volume: 43
Issue: 3
Pages: 221-229
DOI
Related Report
Peer Reviewed
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