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Photodynamic diagnosis and treatment for local multiple-drug-resistant bacterial infection using photosensitizers that preferably accumulate into bacteria.

Research Project

Project/Area Number 23659628
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field General surgery
Research InstitutionNational Defense Medical College

Principal Investigator

MORIMOTO YUJI  防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科専門課程, 准教授 (10449069)

Co-Investigator(Kenkyū-buntansha) KINOSHITA Manabu  防衛医科大学校, 医学教育部医学科専門課程, 准教授 (70531391)
Research Collaborator HAMBLIN Michael R  Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States of America
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords光線力学療法 / 薬剤耐性菌 / 発光細菌 / MRSA / メチレンブルー / 膝関節炎 / 多剤耐性菌 / マウス
Research Abstract

With an established murine intractable MRSA arthritis model using bioluminescent MRSA and resin microparticle, we examined both therapeutic and preventive effect of PDT using methylene blue. Although bioluminescent intensity of MRSA was not diminished immediately after PDT, it was gradually decreased for 6 days after PDT. The therapeutic effect was lost by intravenous administration of anti-neutrophil antibody. Moreover, pre-conditioning PDT on the normal knee joint prevented an exacerbation of MRSA infection of the knee joint. The preventive effect was also lost by intravenous administration of anti-neutrophil antibody. On the other hand, an adjuvant use of antibiotics for MRSA either linezolid or vancomycin exerted no enhancing effect of MB-PDT, suggesting that they might have inhibited the PDT induced accumulating effect of neutrophils into infected lesions.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012 2011 Other

All Journal Article (21 results) (of which Peer Reviewed: 7 results) Presentation (10 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] Linezolid and vancomycin decrease the therapeutic effect of methylene blue-photodynamic therapy in a mouse model of MRSA bacterial arthritis2013

    • Author(s)
      Tanaka M, Mroz P, Dai T, Huang L, Morimoto Y, Kinoshita M, Yoshihara Y, Shinomiya N, Seki S, Nemoto K, Hamblin MR
    • Journal Title

      Photochem Photobiol

      Volume: 89(3) Pages: 679-682

    • Related Report
      2013 Final Research Report
  • [Journal Article] 光線力学療法で感染症を撃退2013

    • Author(s)
      守本祐司
    • Journal Title

      光アライアンス

      Volume: 24 Pages: 1-5

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
  • [Journal Article] 【光線力学診断・治療の最新動向】 PDT における好中球の重要性2013

    • Author(s)
      守本祐司, 田中優砂光, 木下学
    • Journal Title

      日本レーザー医学会誌

      Volume: 34(2) Pages: 133-137

    • Related Report
      2013 Final Research Report
  • [Journal Article] 細菌感染症の新しいアプローチ2013

    • Author(s)
      十河基文, 守本祐司
    • Journal Title

      光殺菌治療. クインテッセンス

      Volume: 32 Pages: 112-123

    • Related Report
      2013 Final Research Report
  • [Journal Article] Linezolid and vancomycin decrease the therapeutic effect of methylene blue-photodynamic therapy in a mouse model of MRSA bacterial arthritis2013

    • Author(s)
      Tanaka, M. Mroz, P. Dai, T. Huang, L. Morimoto, Y. Kinoshita, M. Yoshihara, Y. Shinomiya, N. Seki, S. Nemoto, K. Hamblin, M. R.
    • Journal Title

      Photochem Photobiol

      Volume: 89(3) Issue: 3 Pages: 679-82

    • DOI

      10.1111/php.12040

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] PDTにおける好中球の重要性2013

    • Author(s)
      守本祐司 他
    • Journal Title

      日本レーザー医学会誌

      Volume: 34 Pages: 133-137

    • NAID

      130004893573

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細菌感染症の新しいアプローチ「光殺菌治療」2013

    • Author(s)
      十河基文、守本祐司
    • Journal Title

      クインテッセンス

      Volume: 32 Pages: 112-123

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 光をあてよう2013

    • Author(s)
      守本祐司
    • Journal Title

      Infection Control

      Volume: 22 Pages: 1-1

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation2012

    • Author(s)
      Tanaka M, Mroz P, Dai T, Huang L, Morimoto Y, Kinoshita M, Yoshihara Y, Nemoto K, Shinomiya N, Seki S, Hamblin MR
    • Journal Title

      PloS one

      Volume: 7(6)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Optimal Photosensitizers for Photodynamic Therapy of Infections Should Kill Bacteria but Spare Neutrophils2012

    • Author(s)
      Tanaka M, Kinoshita M, Yoshihara Y, Shinomiya N, Seki S, Nemoto K, Hirayama T, Dai T, Huang L, Hamblin MR, Morimoto Y
    • Journal Title

      Photochem Photobiol

      Volume: 88(1) Pages: 227-232

    • Related Report
      2013 Final Research Report
  • [Journal Article] Photodynamic therapy induces an immune response against a bacterial pathogen2012

    • Author(s)
      Huang YY, Tanaka M, Vecchio D, Garcia-Diaz M, Chang J, Morimoto Y, Hamblin MR
    • Journal Title

      Expert review of clinical immunology

      Volume: 8(5) Pages: 479-494

    • Related Report
      2013 Final Research Report
  • [Journal Article] Type I and Type II mechanisms of antimicrobial photodynamic therapy : an in vitro study on gram-negative and gram-positive bacteria2012

    • Author(s)
      Huang L, Xuan Y, Koide Y, Zhiyentayev T, Tanaka M, Hamblin MR
    • Journal Title

      Lasers Surg Med

      Volume: 44(6) Pages: 490-499

    • Related Report
      2013 Final Research Report
  • [Journal Article] 光線力学療法(PDT) を用いた骨関節手術後感染の予防戦略2012

    • Author(s)
      田中優砂光, 吉原愛雄, 守本祐司, Hamblin M, 根本孝一
    • Journal Title

      整形・災害外科

      Volume: 55 Pages: 75-79

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
  • [Journal Article] 光線力学療法(PDT)の局所細菌感染症への応用 化膿性関節炎モデルを用いた実験的研究2012

    • Author(s)
      田中優砂光, 守本祐司, 木下学, 吉原愛雄, 四ノ宮成祥, 関修司, 斎藤大蔵, 宮崎裕美, Hamblin MR, 根本孝一
    • Journal Title

      防衛医科大学校雑誌

      Volume: 37(4) Pages: 243-256

    • Related Report
      2013 Final Research Report
  • [Journal Article] 光線力学療法(PDT)の生体防御能賦活効果2012

    • Author(s)
      田中優砂光, 木下学, 吉原愛雄, 四ノ宮成祥, 関修司, 根本孝一, 守本祐司
    • Journal Title

      光アライアンス

      Volume: 23 Pages: 42-45

    • Related Report
      2013 Final Research Report
  • [Journal Article] Optimal photosensitizers for photodynamic therapy of infections should kill bacteria but spare neutrophils.2012

    • Author(s)
      Tanaka, M
    • Journal Title

      Photochem. Photobiol.

      Volume: 88 Issue: 1 Pages: 227-232

    • DOI

      10.1111/j.1751-1097.2011.01005.x

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photodynamic therapy induces an immune response against a bacterial pathogen2012

    • Author(s)
      Masamitsu Tanaka et al.
    • Journal Title

      Expert Review of Clinical Immunology

      Volume: 8 Issue: 5 Pages: 479-494

    • DOI

      10.1586/eci.12.37

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 光線力学療法 (PDT) の局所細菌感染症への応用~化膿性関節炎モデルを用いた実験的研究~2012

    • Author(s)
      田中優砂光 他
    • Journal Title

      防衛医科大学校雑誌

      Volume: 37 Pages: 243-256

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photodynamic therapy can induce a protective innate immune response against murine bacterial arthritis via neutrophil accumulation.2012

    • Author(s)
      Tanaka, M
    • Journal Title

      PLoS One

      Volume: 7 Issue: 6 Pages: e39823-e39823

    • DOI

      10.1371/journal.pone.0039823

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photodynamic therapy using intra-articular Photofrin for murine MRSA arthritis : biphasic light dose response for neutrophil-mediated antibacterial effect2011

    • Author(s)
      Tanaka M, Kinoshita M, Yoshihara Y, Shinomiya N, Seki S, Nemoto K, Hamblin MR, Morimoto Y
    • Journal Title

      Lasers Surg Med

      Volume: 43(3) Pages: 221-229

    • Related Report
      2013 Final Research Report
  • [Journal Article] Photodynamic therapy using intra-articular Photofrin for murine MRSA arthritis : biphasic light dose response for neutrophil-mediated antibacterial effect2011

    • Author(s)
      Tanaka, M., Kinoshita, M., Yoshihara, Y., Shinomiya, N., Seki, S., Nemoto, K., Hamblin, M.R., Morimoto, Y.
    • Journal Title

      Lasers Surg Med

      Volume: 43 Issue: 3 Pages: 221-229

    • DOI

      10.1002/lsm.21037

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Bactericidal effect of photodynamic therapy is attenuated by antibiotics2014

    • Author(s)
      Toshikatsu Horiuchi, et al.
    • Organizer
      最先端研究開発支援プログラム (FIRST)「ナノバイオテクノロジーが先導する診断・治療イノベーション」 (NanoBio First) 国際シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Bactericidal effect of photodynamic therapy is attenuated by antibiotics2014

    • Author(s)
      Toshikatsu Horiuchi et al
    • Organizer
      最先端研究開発支援プログラム(FIRST)「ナノバイオテクノロジーが先導する診断・治療イノベーション」 (NanoBio First) 国際シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] リネゾリドおよびバンコマイシンはMRSA関節炎に対する光線力学療法(PDT)の治療効果を減弱させる2013

    • Author(s)
      田中優砂光, 他
    • Organizer
      第23回日本光線力学学会学術講演会
    • Place of Presentation
      旭川市
    • Related Report
      2013 Final Research Report
  • [Presentation] リネゾリドおよびバンコマイシンはMRSA関節炎に対する光線力学療法(PDT)の治療効果を減弱させる2013

    • Author(s)
      田中優砂光 他
    • Organizer
      第23回日本光線力学学会学術講演会
    • Place of Presentation
      旭川市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光線力学の基礎と臨床応用への展望 PDTによる細菌性関節炎への治療ならびに予防2012

    • Author(s)
      守本祐司, 他
    • Organizer
      第22回日本光線力学学会学術講演会
    • Place of Presentation
      つくば市
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Invited
  • [Presentation] 光線力学療法により細菌感染症を撃退2012

    • Author(s)
      守本祐司, 他
    • Organizer
      東京都国立市医師会
    • Place of Presentation
      東京都
    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Invited
  • [Presentation] Photodynamic therapy can induce non-specific protective immunity against a bacterial infection2012

    • Author(s)
      Masamitsu Tanaka, et al.
    • Organizer
      Photonics West 2012_BiOS
    • Place of Presentation
      San Jose, CA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Photodynamic therapy can induce a protective immune response against a bacterial pathogen2012

    • Author(s)
      Michael R Hamblin, et al.
    • Organizer
      14th Congress of the European Society for Photobiology
    • Place of Presentation
      Geneva, Switzerland
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Photodynamic therapy can induce non-specific protective immunity against a bacterial infection2012

    • Author(s)
      Masamitsu Tanaka et al.
    • Organizer
      Photonice West 2012_BiOS
    • Place of Presentation
      San Jose, CA, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Photodynamic therapy can induce a protective immune response against a bacterial pathogen.2011

    • Author(s)
      Michael R Hamblin et al.
    • Organizer
      14th Congress of the European Society for Photobiology(招待講演)
    • Place of Presentation
      Geneva, Switzerland
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://ndmc-ipb.browse.jp/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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