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Analysis of regulatory mechanism for liver regeneration by various types of cell death(autophagy and apoptosis)

Research Project

Project/Area Number 23659631
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionHokkaido University

Principal Investigator

OZAKI Michitaka  北海道大学, 大学院・保健科学研究院, 教授 (80256510)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsオートファジー / アポトーシス / p62/SQSTM1 / Nrf-2 / p62 / 細胞死 / 肝再生
Research Abstract

In order to examine the regulatory role of autophagy and p62/SQSTM1 in fatty liver regeneration, we studied steatotic cell death (autophagy and apoptosis) in liver/hepatocytes.(1) Establishment of evaluation method for autophagy in cell/liver tissue. We tried to quantitatively evaluate autophagy of cells and liver tissue by expressions of LC3 and p62/SQSTM1, and electron micrograph.(2) We studied the regulatory mechanism of p62/SQSTM1 expression and its relation to apoptosis/autophagy and oxidative stress. p62/SQSTM1 expression was regulated by autophagy specifically, not by apoptosis, but also regulated positively by PI3-K/PDK/Akt-dependent signals. p62/SQSTM1 was also involved in suppression of cellular oxidative stress and injury. In mouse liver, p62/SQSTM1 increased cellular anti-oxidative molecules such as SOD, Ref-1 and Catalase through Nrf-2 and suppressed oxidative stress.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2013 2012 2011

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (17 results)

  • [Journal Article] Rapid Methods of Detecting the Target Molecule in immunohistology using a Bioluminescence Probe.2013

    • Author(s)
      Chun Wu, Ke-Yong Wang, Xin Guo, Masanori Sato, Michitaka Ozaki, Shyohei Shimajiri, Yoshihiro Ohmiya, Yasuyuki Sasaguri.
    • Journal Title

      Luminescence: The Journal of Biological and Chemical Luminescence.

      Volume: 28(1) Pages: 38-438

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo monitoring of liver damage by caspase-3 probe.2012

    • Author(s)
      Michitaka Ozaki, Sanae Haga, Takeaki Ozawa.
    • Journal Title

      Theranostics.

      Volume: 2(2) Pages: 207-214

    • NAID

      10031131094

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo monitoring of liver damage by caspase-3 probe.2012

    • Author(s)
      Michitaka Ozaki, Sanae Haga, Takeaki Ozawa.
    • Journal Title

      Theranostics

      Volume: 2 Pages: 207-214

    • NAID

      10031131094

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rapid Methods of Detecting the Target Molecule in immunohistology using a Bioluminescence Probe2012

    • Author(s)
      Chun Wu, Ke-Yong Wang, Xin Guo, Masanori Sato, Michitaka Ozaki, Shyohei Shimajiri, Yoshihiro Ohmiya, Yasuyuki Sasaguri
    • Journal Title

      Luminescence : The Journal of Biological and Chemical Luminescence

      Volume: 28(1) Issue: 1 Pages: 38-43

    • DOI

      10.1002/bio.2333

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inhibition of macrophage activation and suppression of graft rejection by DTCM-glutarimide, a novel piperidine derived from the antibiotic 9-methylstreptimidone.2011

    • Author(s)
      Masatoshi Takeiri, Miyuki Tachibana, Ayumi Kaneda, Ayumi Ito, Yuichi Ishikawa, Shigeru Nishiyama, Ryoichi Goto, Kenichiro Yamashita, Susumu Shibasaki, Gentaro Hirokata, Michitaka Ozaki, Satoru Todo, and Kazuo Umezawa.
    • Journal Title

      Inflammation Research

      Volume: 60 Pages: 879-888

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 移植臓器の非侵襲的モニタリング法の開発2011

    • Author(s)
      尾崎倫孝、芳賀早苗、森田直樹、深井原、藤堂省、小澤岳昌、近江谷克祐
    • Journal Title

      Organ Biology

      Volume: 18(1) Pages: 134-140

    • NAID

      130004709996

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 移植臓器の非侵襲的モニタリングの開発2011

    • Author(s)
      尾崎倫孝
    • Journal Title

      Organ Biology

      Volume: 18 Pages: 134-140

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 「p62/STSQM1 plays a central role in liver injury in steatotic liver during regeneration.」2012

    • Author(s)
      芳賀 早苗、小澤 岳昌、森田 直樹、梅澤 一夫、藤堂 省、尾崎 倫孝
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Year and Date
      2012-12-06
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] p62/STSQM1 plays a central role in liver injury in steatotic liver during regeneration2012

    • Author(s)
      芳賀 早苗、小澤 岳昌、森田 直樹、梅澤 一夫、藤堂 省、尾崎 倫孝
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Year and Date
      2012-12-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Bio-imaging of surgical stress: Dynamic analysis of liver oxidative stress and damage"2012

    • Author(s)
      N. Morita, S. Haga, T. Ozawa, S. J. Remington, M. Ozaki
    • Organizer
      17th International Symposium on BioluminescenceandChemiluminescence(ISBC2012)
    • Place of Presentation
      2DeltaGuelphHotel&ConferenceCentre,Guelph,Ontario,Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] 「虚血/再灌流傷害における高血糖および脂肪化病態肝の反応性の相違」2012

    • Author(s)
      芳賀 早苗、小澤 岳昌、森田 直樹、阿部 理一郎、藤堂 省、尾崎 倫孝
    • Organizer
      第19回肝細胞研究会
    • Place of Presentation
      札幌医科大学臨床教育研究棟講堂
    • Related Report
      2012 Annual Research Report
  • [Presentation] "Bio-imaging of surgical stress: Dynamic analysis of liver oxidative stress and damage"2012

    • Author(s)
      N. Morita, S. Haga, T. Ozawa, S. J. Remington, M. Ozaki
    • Organizer
      17th International Symposium on BioluminescenceandChemiluminescence(ISBC2012)
    • Place of Presentation
      DeltaGuelphHotel&ConferenceCentre,Guelph,Ontario,Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] 「生体イメージング法による細胞・臓器のリアルタイム評価法の開発」2011

    • Author(s)
      尾崎倫孝
    • Organizer
      第38回日本臓器保存生物医学会学術集会
    • Place of Presentation
      江陽グランドホテル(仙台)
    • Related Report
      2012 Final Research Report
  • [Presentation] Age-associated p66Shc induced redox-dependent liver damage and impaired regeneration after hepatectomy in aged mice2011

    • Author(s)
      Michitaka Ozaki, Sanae Haga, Kaikobad Irani, Naoki Morita, Yoshimi Kaneshima, Takeaki Ozawa
    • Organizer
      62th Annual meeting of AASLD
    • Place of Presentation
      San Francisco, USA (Moscone West Convention)
    • Related Report
      2012 Final Research Report
  • [Presentation] 脂肪肝における Fas 過剰発現および細胞内酸化的ストレスによる肝再生不全の検討2011

    • Author(s)
      芳賀早苗,森田直樹,青山貴子,小澤岳昌Remington S.James,阿部理一郎,尾崎倫孝
    • Organizer
      第84回日本生化学会大 会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2012 Final Research Report
  • [Presentation] Bio-imaging of Surgical Stresses-Dynamic Analyses of Liver Oxidative Stress and Damage2011

    • Author(s)
      M. Ozaki, S. Haga, T. Ozawa, N. Morita, Y. Kaneshima, J. Remington
    • Organizer
      5THEuropean Conference of the International Federation for Medical and Biological Engineering Budapest Congress & World Trade Center,
    • Place of Presentation
      Budapest, Hungary
    • Related Report
      2012 Final Research Report
  • [Presentation] CollagenVitrigel Sheet'as a Novel Drug Delivery Bio-material2011

    • Author(s)
      Sanae Haga, Toshiaki Takezawa, Takeaki Ozawa, James Remington, Naoki Morita and Michitaka Ozaki
    • Organizer
      5THEuropean Conference of the International Federation for Medical and Biological Engineering Budapest Congress & World Trade Center
    • Place of Presentation
      Budapest, Hungary
    • Related Report
      2012 Final Research Report
  • [Presentation] レミントン ジェームズ、尾崎 倫孝:「脂肪肝における肝再生不全機序の解析」2011

    • Author(s)
      芳賀早苗、森田直樹、小澤岳昌
    • Organizer
      第18回肝細胞研究会
    • Place of Presentation
      東京ガーデンパレス
    • Related Report
      2012 Final Research Report
  • [Presentation] 生体イメージング法による細胞・臓器のリアルタイム評価法の開発2011

    • Author(s)
      尾崎倫孝
    • Organizer
      第38回日本臓器保存生物医学会学術集会
    • Place of Presentation
      江陽グランドホテル(仙台市)
    • Related Report
      2011 Research-status Report
  • [Presentation] Age-associated p66Shc induced redox-dependent liver damage and impaired regeneration after hepatectomy in aged mice.2011

    • Author(s)
      Michitaka Ozaki
    • Organizer
      62th Annual meeting of AASLD
    • Place of Presentation
      Moscone West Convention Center(San Francisco, USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] 脂肪肝におけるFas過剰発現および細胞内酸化的ストレスによる肝再生不全の検討2011

    • Author(s)
      芳賀 早苗
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Bio-imaging of Surgical Stresses-Dynamic Analyses of Liver Oxidative Stress and Damage.2011

    • Author(s)
      M. Ozaki
    • Organizer
      5TH European Conference of the International Federation for Medical and Biological Engineering
    • Place of Presentation
      Budapest Congress & World Trade Center(Budapest, Hungary)
    • Related Report
      2011 Research-status Report
  • [Presentation] 'Collagen Vitrigel Sheet’as a Novel Drug Delivery Bio-material.2011

    • Author(s)
      Sanae Haga
    • Organizer
      5TH European Conference of the International Federation for Medical and Biological Engineering
    • Place of Presentation
      Budapest Congress & World Trade Center(Budapest, Hungary)
    • Related Report
      2011 Research-status Report
  • [Presentation] 脂肪肝における肝再生不全機序の解析2011

    • Author(s)
      芳賀 早苗
    • Organizer
      第18回肝細胞研究会
    • Place of Presentation
      東京ガーデンパレス(東京)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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