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Creation of the HCV-infectable mouse as a small animal model

Research Project

Project/Area Number 23659645
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

GO Isshin  浜松医科大学, 医学部, 助教 (60283363)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsC 型肝炎ウイルス / HCV 感染性マウス / ウイルス受容体 / 感染因子 / C型肝炎ウイルス / 感染 / 侵入 / CD81 / SCAVRB1 / Claudin 1 / Occludin / NPC1L1 / HCV受容体
Research Abstract

In order to make the transgenic mouse to be infected by HCV, we created the transgenic (Tg) mouse in which human four factors were expressed in the mouse hepatocytes. After confirming that HCV E2 protein bound to the hepatocytes of Tg mouse, we injected HCV from the patient serum. We measured the viral genome RNA copy number in the blood by the qRT-PCR method. Result showed that HCV cannot infect to the hepatocytes of Tg mouse. We investigated whether mouse inhibitory proteins inhibit the entry of HCV into hepatocytes and/or another essential human factor is necessary for the entry of HCV. Results suggest that the mouse Cd81 and Ocln proteins inhibit the entry process and that human NPC1L1 protein enhances the entry of the virus.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (5 results)

All 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results)

  • [Journal Article] Baicalin and scutellarin are proteasome inhibitors that specifically target chymotrypsin-like catalytic activity.2013

    • Author(s)
      Yi-Xin Wu, Eiji Sato, Wataru Kimura, Naoyuki Miura
    • Journal Title

      Phytotherapy Res

      Volume: in press Issue: 9 Pages: 1362-1367

    • DOI

      10.1002/ptr.4878

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] miR-1915 inhibits Bcl-2 to modulate multidrug resistance by increasing drug-sensitivity in human colorectal carcinoma cells2013

    • Author(s)
      Xu K, Liang X, Cui D, Wu Y-X, Shi W, Liu J
    • Journal Title

      Mol Carcinogesis

      Volume: 52 Issue: 1 Pages: 70-78

    • DOI

      10.1002/mc.21832

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Liver tumor formation by a mutant retinoblastoma protein in the transgenic mice is caused by an up-regulation of c-Myc target genes2012

    • Author(s)
      Wang B, et al
    • Journal Title

      Biochemica Biophysica Res Commun

      Volume: 417 Issue: 1 Pages: 601-606

    • DOI

      10.1016/j.bbrc.2011.12.014

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene cloning and expression of a novel hypoglycaemic peptide from Momordica charantia.2011

    • Author(s)
      Wang BL, Zhang WJ, Zhao J, Wang FJ, Fan LQ, Wu YX, Hu ZB
    • Journal Title

      J Sci Food Agric

      Volume: 91 Issue: 13 Pages: 2443-8

    • DOI

      10.1002/jsfa.4485

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis of immunomagnetic nanoparticles and their use for breast cancer cell separation.2011

    • Author(s)
      Liang X et al.
    • Journal Title

      Adv Sci Lett

      Volume: 4 Issue: 11 Pages: 3403-3407

    • DOI

      10.1166/asl.2011.1903

    • Related Report
      2011 Research-status Report
    • Peer Reviewed

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Published: 2011-08-05   Modified: 2019-07-29  

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