Creation of the HCV-infectable mouse as a small animal model
| Project/Area Number |
23659645
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
GO Isshin 浜松医科大学, 医学部, 助教 (60283363)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | C 型肝炎ウイルス / HCV 感染性マウス / ウイルス受容体 / 感染因子 / C型肝炎ウイルス / 感染 / 侵入 / CD81 / SCAVRB1 / Claudin 1 / Occludin / NPC1L1 / HCV受容体 |
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| Research Abstract |
In order to make the transgenic mouse to be infected by HCV, we created the transgenic (Tg) mouse in which human four factors were expressed in the mouse hepatocytes. After confirming that HCV E2 protein bound to the hepatocytes of Tg mouse, we injected HCV from the patient serum. We measured the viral genome RNA copy number in the blood by the qRT-PCR method. Result showed that HCV cannot infect to the hepatocytes of Tg mouse. We investigated whether mouse inhibitory proteins inhibit the entry of HCV into hepatocytes and/or another essential human factor is necessary for the entry of HCV. Results suggest that the mouse Cd81 and Ocln proteins inhibit the entry process and that human NPC1L1 protein enhances the entry of the virus.
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Report
(3 results)
Research Products
(5 results)
