Circulating Tumor Cell of Gastrointestinal Stromal Tumor

• Project/Area Number: 23659651
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Okayama University
• Principal Investigator: UNO Futoshi 岡山大学, 岡山大学病院, 助教 (90572503)
• Co-Investigator(Renkei-kenkyūsha): FUJIWARA Toshiyoshi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (00304303) ; KAGAWA Shunsuke 岡山大学, 岡山大学病院, 講師 (00362971) ; NISHIZAKI Masahiko 岡山大学, 岡山大学病院, 助教 (30379789) ; NAGASAKA Takeshi 岡山大学, 岡山大学病院, 助教 (30452569) ; TAZAWA Hiroshi 岡山大学, 岡山大学病院, 助教 (90415513)
• Research Collaborator: SHIGEYAU Kunitoshi 岡山大学, 大学院・医歯薬学総合研究科, 大学院生 ; HASHIMOTO Yuuri 岡山大学, 大学院・医歯薬学総合研究科, 大学院生
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 消化管間質腫瘍(GIST) / 血中浮遊腫瘍細胞(CTC) / 遺伝子解析

Research Abstract

We detected circulating tumor cells (CTCs) in the peripheral blood of Gastrointestinal Stromal Tumor (GIST) patients after some fundamental research useing a green fluorescent protein (GFP)-expressing attenuated adenovirus-5 vector, in which the hTERT promoter regulates viral replication (TelomeScan). CTCs were identified in samples from 6 of 12 patients with GIST. These results suggest that CTSs detection with our method might be a new bio-marker for GIST which frequently accompanied liver metastases.

Research Products

• [Presentation] 腫瘍特異的制限増殖型アデノウイルスを用いた血液循環腫瘍細胞の遺伝子解析技術の開発 (2012)
  • Author(s): 重安邦俊,橋本悠里,宇野 太 ,永坂岳司,田澤 大,香川俊輔,水口裕之,浦田泰生,藤原俊義
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロントイン札幌
  • Year and Date: 2012-09-19
• [Presentation] New method for investigating genetic mutations of circulating tumor cells using a telomerase-specific replication-competent adenovirus (2012)
  • Author(s): Shigeyasu K, Hashimoto Y, Kagawa S, Uno F, Tazawa H, Nagasaka T, Kyo S, Mizuguchi H, Urata Y, Fujiwara T
  • Organizer: 第18回日本遺伝子治療学会年次学術集会
  • Place of Presentation: ホテル熊本テルサ
  • Year and Date: 2012-06-29
• [Presentation] 消化管間質腫瘍における血中浮遊腫瘍細胞検出の試み (2012)
  • Author(s): 宇野太、重安邦俊、永坂岳司、香川俊輔、西崎正彦、岸本浩行、藤原俊義
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ 国際会議場
  • Year and Date: 2012-04-13
• [Presentation] A highly sensitive detection system of genetic alterations in circulating tumor cells using a telomerase-specific replication-competent adenovirus (2012)
  • Author(s): Shigeyasu K, Hashimoto Y, Morikawa T, Mori Y, Sun DS, Kagawa S, Uno F, Tazawa H,Nagasaka T, Kyo S, Mizuguchi H, Urata Y, Fujiwara T
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: 米国シカゴ
  • Year and Date: 2012-04-02
• [Presentation] 消化管間質腫瘍における血中浮遊腫瘍細胞検出の試み (2012)
  • Author(s): 宇野 太
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張メッセ 国際会議場
• [Presentation] A highly sensitive detection system of genetic alterations in circulating tumor cells using a telomerase-specific replication-competent adenovirus (2012)
  • Author(s): Shigeyasu K
  • Organizer: AACR Annual Meeting
  • Place of Presentation: 米国シカゴ